Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 275,668 G→A G115E (GGA→GAA)  mmuP → S‑methyl‑L‑methionine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913275,6680GA96.4% 89.3 / NA 29G115E (GGA→GAA) mmuPS‑methyl‑L‑methionine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (13/14);  minor base C (1/0);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTCAATACCGGGTACATCATTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGGATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGG  >  NC_000913/275422‑275891
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               
actacAATACCGGGTACATCATTCCCACCACGGGAGCGGCGGGAACGCTGCTGGCCTATCGGATGGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCGGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTCCGGGGTATACCGTGGCCTGGCTTTAGTGGCTGACCTGGACCGTGGCGGTGGGTTCGAGCGTTACCGCCGCTGAAtt                                                                                                                                                                                                                              <  2:256593/246‑1 (MQ=255)
   tcAATACCGGGTACAGCATTTCCACCATTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCGGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACTTTTAGGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCGGGCTTTACGGGCTGACCTGGACCGTGGCGCTGGGTGCGAGTTTTCCCGCCGCTGGATTCt                                                                                                                                                                                                                            <  2:250906/249‑1 (MQ=255)
     aaTACGGGGTACATCATTTCCCCCACTGGGGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGGGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATCCCGTGGCCTGGCTTTACTGGCTGACCTGGACCGGGGCGCGGGGTTCGAGCTTTACCGCCGCTGAATTCTatat                                                                                                                                                                                                                        <  2:77810/251‑1 (MQ=255)
                                   gcggcgGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCGGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCGGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATg                                                                                                                                                                                          <  1:351388/251‑1 (MQ=255)
                                     ggcggGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATggg                                                                                                                                                                                        >  1:125476/1‑251 (MQ=255)
                                        gggACCGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCGGTGTCTGGGGGAGCTGTCGGTCGCTATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCTCTATCTTGGGCCGGCTACGGGGTATACCGTGGCCGGGCTTTACGGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCt                                                                                                                                                                                     <  2:75051/251‑1 (MQ=255)
                                               ctgctgGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCgt                                                                                                                                                                              >  2:405577/1‑251 (MQ=255)
                                                 gttgGCCTATCTGATTGGTGCGCGGGTGGTCTGGCGGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTCCCGGGTATACCGTGGCCTGGCTTTACGGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGGTTCCACAGGTGCCGGTATGGGTCTGGTGCgt                                                                                                                                                                              <  1:113763/247‑1 (MQ=255)
                                                  ggggcttATCTGATTGGTGCGCGGGTGGTCTGGCTGTTTATGCAGTGTCGGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGGGTTTCACGTTTATGCCGCGCGCTATCTTGGCCGGGCTACGGGGTATCCCGTGGCCTGGTTTTACTGGCTGCCCGGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCgtggt                                                                                                                                                                           <  2:125476/245‑1 (MQ=255)
                                                         acctgattgttggtcTGGTGGTCTGGCTGGTTATGCATTTTCTGGGCGAGCTTTGGGTCGCGATGCCGGAGACCGGAGAGTTTCACGTTTGTGCCGCGCGCTATCTTGGTCCGTCTACCGGGTATCCGGTGACGTGGCTTTACTGGCTGTCCTGGACGGTGGCGCTGGTTTCGATTTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCt                                                                                                                                                                      <  1:275249/235‑1 (MQ=255)
                                                            ggATTGGTGCGCGGGTGTTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTCCCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGCCCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGcgcg                                                                                                                                                                 <  1:55883/250‑1 (MQ=255)
                                                               ttGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATCCCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGat                                                                                                                                                               <  1:158577/250‑1 (MQ=255)
                                                                 ggTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCAAGTTTATGCCGAGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGCATTCTGTATGCAGTACTGGTTTCCTCAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGAGCGattat                                                                                                                                                            >  1:395261/1‑251 (MQ=255)
                                                                    gcgcTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTAtttt                                                                                                                                                         >  1:47268/1‑251 (MQ=255)
                                                                           tggtCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTg                                                                                                                                                  >  1:70226/1‑251 (MQ=255)
                                                                                   ctggTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGATCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTAt                                                                                                                                          >  2:289753/1‑251 (MQ=255)
                                                                                    tggTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGCGCTTTACCGCCGATGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTAtc                                                                                                                                         >  2:353487/1‑251 (MQ=255)
                                                                                                    gggCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTCCCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCttttt                                                                                                                           <  1:230838/249‑1 (MQ=255)
                                                                                                            tGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCAcg                                                                                                                                   >  1:404410/1‑233 (MQ=255)
                                                                                                                 gtcgCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGtt                                                                                                            >  1:402544/1‑251 (MQ=255)
                                                                                                                                 ccGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTc                                                                                            <  1:130543/251‑1 (MQ=255)
                                                                                                                                     aGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCaa                                                                                          >  2:69855/1‑249 (MQ=255)
                                                                                                                                              cGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACt                                                                                >  1:346538/1‑250 (MQ=255)
                                                                                                                                                                       ccGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTgg                                                      >  1:116261/1‑251 (MQ=255)
                                                                                                                                                                                       ccGTGGCCGGGCTTTCCTGGCTGGCCTGGACCGTGGGGCTGTGTTCGGGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGTTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGTTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGc                                      <  2:167722/251‑1 (MQ=255)
                                                                                                                                                                                               tGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCCCAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTcc                              >  2:259909/1‑251 (MQ=255)
                                                                                                                                                                                                        tGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGCGGGGCGGCGATTTTCGGCTTTATTCCGATGCAgg                      >  2:284161/1‑250 (MQ=255)
                                                                                                                                                                                                                 gggcccGTGGCGCTGGGTGCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGcc            <  1:115879/247‑1 (MQ=255)
                                                                                                                                                                                                                           cgcggggTTCGAGCTTTACCGCCGCTGAATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCgggg  <  1:317044/247‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               
TCTTCAATACCGGGTACATCATTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACCTGGACCGTGGCGCTGGGTTCGAGCTTTACCGCCGCTGGATTCTGTATGCAGTACTGGTTTCCACAGGTGCCGGTATGGGTCTGGTGCGTGGTGTTCTGCGCGATTATTTTTGGTCTGAATGTTATCTCCACGCGCTTTTTTGCCGAAGGGGAGTTCTGGTTCTCGCTGGTCAAAGTGGTCACTATCATCGCCTTTATCATCCTCGGTGGGGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGG  >  NC_000913/275422‑275891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑