Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,761,230	G→A	S7F (TCC→TTC)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,761,230	0	G	A	100.0%	172.1 / NA	47	S7F (TCC→TTC)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/23);  total (24/23)

CCCGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGGAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGACG  >  NC_000913/3760987‑3761473                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    cccGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCATCCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGTCTGCCACGAATCTCTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGt                                                                                                                                                                                                                                              <  1:14947/251‑1 (MQ=255)                aCCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAgg                                                                                                                                                                                                                               >  1:91163/1‑251 (MQ=255)                  cGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCCCTCCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGTTGCGTCGATCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGaa                                                                                                                                                                                                                             <  1:262928/251‑1 (MQ=255)                     aCGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTAGCGCTCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTGGGTGGTCATAGGAAAcc                                                                                                                                                                                                                          <  1:145647/251‑1 (MQ=255)                           cgcgcTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCTTTTCGGTTGTCATAGGAAACCTGGCTg                                                                                                                                                                                                                    >  1:286209/1‑251 (MQ=255)                            gcgcTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGa                                                                                                                                                                                                                   <  1:143895/251‑1 (MQ=255)                              gcTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATc                                                                                                                                                                                                                 >  1:32322/1‑251 (MQ=255)                                   gcgcTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCaa                                                                                                                                                                                                               <  1:146708/248‑1 (MQ=255)                                    cgcTTCTTTCGTCACCCGGGCATCGACTTCTTACGCACAGTTTTCACACCACGCACGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGcc                                                                                                                                                                                                           >  2:252899/1‑251 (MQ=255)                                       ttcttTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCACCAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCctc                                                                                                                                                                                                        >  1:269291/1‑251 (MQ=255)                                           ttCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCATCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCctct                                                                                                                                                                                                       <  1:237211/248‑1 (MQ=255)                                                 aaCCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGTCTGATCAAGGCCCTCTCACACGgag                                                                                                                                                                                              <  2:133274/251‑1 (MQ=255)                                                    cGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGgaga                                                                                                                                                                                             >  1:55223/1‑249 (MQ=255)                                                       gCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGc                                                                                                                                                                                        >  1:243604/1‑251 (MQ=255)                                                       gCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGc                                                                                                                                                                                        >  1:359943/1‑251 (MQ=255)                                                       gCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGc                                                                                                                                                                                        <  2:264159/251‑1 (MQ=255)                                                          tCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGCATCACTTCTCGCGCTTCGTCGAACATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAGAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGtt                                                                                                                                                                                     >  2:203114/1‑251 (MQ=255)                                                                   tGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATaa                                                                                                                                                                             <  1:150450/250‑1 (MQ=255)                                                                     cgcCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAAcc                                                                                                                                                                           >  2:263960/1‑250 (MQ=255)                                                                        ccAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACgg                                                                                                                                                                       >  1:348251/1‑251 (MQ=255)                                                                               tCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAAcg                                                                                                                                                                                                                                                 >  1:12682/1‑170 (MQ=255)                                                                                  caccacGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGt                                                                                                                                                             >  1:294140/1‑251 (MQ=255)                                                                                               gCGTCTTGCTGCCCCGAATCACTTCTCGCGCGTCGTCGAGCATCTGACGCAACAATTCCTCCACGCGGGTGTGACCATAAGTAGCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGATGAAACGCTTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCTCTCTCACACGGAGAAGGGCGGTTAACATAACCACGGATTGTAACGGGAGATGGGTCagg                                                                                                                                                <  2:367345/251‑1 (MQ=255)                                                                                                    tGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTg                                                                                                                                                                                                                    >  2:86416/1‑178 (MQ=255)                                                                                                    tGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTg                                                                                                                                                                                                                    <  1:86416/178‑1 (MQ=255)                                                                                                     ggCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACa                                                                                                                                          <  1:52561/251‑1 (MQ=255)                                                                                                     ggCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCGCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAggagga                                                                                                                                            <  2:229582/249‑1 (MQ=255)                                                                                                                   aCTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCa                                                                                                                              >  2:58844/1‑249 (MQ=255)                                                                                                                          gcgcTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAACAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTgg                                                                                                                     >  1:121134/1‑251 (MQ=255)                                                                                                                                            gACGCAACAATTCCACCACGCGGGTGTGACCATAAGTAGCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCAGCAAGCCTTTGGCGGTTCGGTGCGAg                                                                                                       <  2:32322/247‑1 (MQ=255)                                                                                                                                                  acaaTTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTc                                                                                             <  2:387548/251‑1 (MQ=255)                                                                                                                                                         caccacGCGGGTGTGACCATAAGCATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCt                                                                                       >  1:155057/1‑250 (MQ=255)                                                                                                                                                          cccacGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGCTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTaa                                                                                     <  2:401846/250‑1 (MQ=255)                                                                                                                                                                            tAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGtt                                                                   >  1:238307/1‑251 (MQ=255)                                                                                                                                                                             aaGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGttt                                                                  >  1:299169/1‑251 (MQ=255)                                                                                                                                                                             aaGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGGAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGtt                                                                   <  1:53348/250‑1 (MQ=255)                                                                                                                                                                             aaGTATCACGCAAAGAAAGGAAGGAGCTAGCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGtt                                                                   <  2:135995/250‑1 (MQ=255)                                                                                                                                                                              aGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTg                                                                 <  2:363405/251‑1 (MQ=255)                                                                                                                                                                                 aTCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGAc                                                               <  1:14098/250‑1 (MQ=255)                                                                                                                                                                                    aCGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAg                                                           <  2:148023/251‑1 (MQ=255)                                                                                                                                                                                       cAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAgtgt                                                        <  1:83436/251‑1 (MQ=255)                                                                                                                                                                                                         aTCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCa                                       >  1:130463/1‑250 (MQ=255)                                                                                                                                                                                                         aTCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCa                                       >  1:4846/1‑250 (MQ=255)                                                                                                                                                                                                                                  ggAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTg              >  1:313814/1‑250 (MQ=255)                                                                                                                                                                                                                                            tataGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGAc   >  1:48292/1‑251 (MQ=255)                                                                                                                                                                                                                                             ataGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGAc   <  2:55223/250‑1 (MQ=255)                                                                                                                                                                                                                                             ataGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGACg  >  1:33169/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CCCGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCACGCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGGAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCTAAACAGGTTTTCGACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGACG  >  NC_000913/3760987‑3761473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑