Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	179.4 / NA	52	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/28);  total (24/28)

GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCA  >  NC_000913/3387799‑3388287                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                           gATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTACTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACAACAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGAGCCCCAGTCCGGCAATACAGCCAATAAATGTGACGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcggcg                                                                                                                                                                                                                                                     <  2:139193/250‑1 (MQ=255)        gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTTTTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATTGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAt                                                                                                                                                                                                                                             <  1:178927/251‑1 (MQ=255)        gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAt                                                                                                                                                                                                                                             <  1:178941/251‑1 (MQ=255)         cgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATc                                                                                                                                                                                                                                            <  2:345569/251‑1 (MQ=255)                                              tATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCt                                                                                                                                                                                                       >  1:90348/1‑251 (MQ=255)                                                  cGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGaa                                                                                                                                                                                                   <  1:367613/251‑1 (MQ=255)                                                      ccAGCGAGGATATCCAGGTAAAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATAACCAGTCCGGCAATACAGCCAATAGATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCATGAAAGGCCGGACCGGCTGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGa                                                                                                                                                                                               <  2:373699/251‑1 (MQ=255)                                                        aGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGaaa                                                                                                                                                                                             <  2:98836/251‑1 (MQ=255)                                                                    cAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAg                                                                                                                                                                                 <  1:245544/251‑1 (MQ=255)                                                                          cAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGcc                                                                                                                                                                             >  2:217283/1‑249 (MQ=255)                                                                             aaaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTAc                                                                                                                                                                        >  2:96992/1‑251 (MQ=255)                                                                                    cccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCg                                                                                                                                                                 >  1:13496/1‑251 (MQ=255)                                                                                     ccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCgg                                                                                                                                                                >  2:267581/1‑251 (MQ=255)                                                                                           aCAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCa                                                                                                                                                          <  1:217283/251‑1 (MQ=255)                                                                                                 cacCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAACGCCAACAAACAGCGCCAGTACAATGGCGGTCTCCAG‑gtttt                                                                                                                                                   >  2:134547/1‑250 (MQ=255)                                                                                                          catcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAg                                                                                                                                           <  1:96992/250‑1 (MQ=255)                                                                                                          catcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGc                                                                                                                                          <  2:271252/251‑1 (MQ=255)                                                                                                                 aatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑Aatat                                                                                                                                   <  1:278345/250‑1 (MQ=255)                                                                                                                 aatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑Aatat                                                                                                                                   <  1:283567/250‑1 (MQ=255)                                                                                                                          cgGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCAAACAAATGTGCCGATGATGCGCAAAAAGCCCCGATCACGAATACAGCCAGAATACGGTCCACCACCCGCAGCCACGGCCGGACCGGCGGCAACAACCGCCCCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGCAGCGAAAGCCCACAAACAGCGCCAGTACACAAGCGGTCGCCAGCTTTTAC‑GCAAAACG‑Aatatagtagttagg                                                                                                                         >  1:204039/1‑241 (MQ=255)                                                                                                                           ggATCATCGCAATGAGGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCa                                                                                                                        <  2:81566/251‑1 (MQ=255)                                                                                                                             atcatcGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCACAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAt                                                                                                                      <  1:400797/251‑1 (MQ=255)                                                                                                                                  cGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑Ggag                                                                                                                 <  1:307697/250‑1 (MQ=255)                                                                                                                                      atgatgATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCCG‑TTTTTCCGCAAAACG‑AATATGTTGGTTTGCAAT‑GGGG‑aaaa                                                                                                            >  2:262693/1‑250 (MQ=255)                                                                                                                                        gatgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATAc                                                                                                         <  1:20947/251‑1 (MQ=255)                                                                                                                                         atgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGCTGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGCG‑AAAATAcc                                                                                                        >  1:24230/1‑251 (MQ=255)                                                                                                                                           gatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCa                                                                                                      <  1:412666/251‑1 (MQ=255)                                                                                                                                            atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑GTTTAACGCAAAGAG‑AAAATGGTGGGTAGCAAT‑GGAGGAAAAAAccc                                                                                                       >  2:150317/1‑250 (MQ=255)                                                                                                                                                  aGTCCGGCAATACAGCCAATAAATGTGCCGATGAAGCGCAAAAAGCCACGATAGCGCATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACCATCGCCGCTGTCCGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACCATGGCGGTCGCCCG‑TTTTACCGCAAAACGCAAT‑TGTTGGTTAGCAAT‑GGAG‑AAAATACCCACCGTGAt                                                                                               >  1:256645/1‑251 (MQ=255)                                                                                                                                                           aTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGGGATTAACCaaa                                                                                       >  1:371360/1‑250 (MQ=255)                                                                                                                                                                        aTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGAt                                                                         <  1:217382/251‑1 (MQ=255)                                                                                                                                                                        aTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAC‑TTTTACCGCAAAGCG‑AAAAATTTGGTTAGCAATCGGGG‑AAAA‑ACCCACCGTGATTAACCACACTCaacaccgcgcg                                                                         >  2:181445/1‑241 (MQ=255)                                                                                                                                                                            gCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAAGACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCTGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGTTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGc                                                                     <  1:263961/251‑1 (MQ=255)                                                                                                                                                                                atgatgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAG‑TTTTACCGCAAAACG‑AATATGTTGGTTAGCCAT‑GGGG‑AAAAAAACCATCGCGATTAAACAAAATCAccccagcgagtggccact                                                                 >  2:213609/1‑234 (MQ=255)                                                                                                                                                                                 tgatgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCCAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCAACGTGATTAACCAAACTCACGCAGGCGATggggccttt                                                                >  2:225533/1‑245 (MQ=255)                                                                                                                                                                                  gatgCGCAAATAGCCACGAGAGCAAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACATACAGCGCCAGGACAATGGCGGTCGCCAG‑TGTTACCGCAAAGCG‑AATATGTTGGTCATCAAT‑GGAG‑AAAATACCCATCATGATTAGCCAAACTCCCGCAGGCGATGGGCCAttt                                                                <  2:219967/250‑1 (MQ=255)                                                                                                                                                                                  gatgCGCAAAACGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCCAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCCCCAG‑TTTTTCCGCCAAACG‑AATATGTTGGTTCGCCAT‑GGAG‑GAAAAACCCCTCGTGATTAACCCAACTCACGCCAGCGCTGGGCCAtttt                                                               >  2:273402/1‑251 (MQ=255)                                                                                                                                                                                     gcgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTAc                                                             <  1:256065/250‑1 (MQ=255)                                                                                                                                                                                         aaaaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑GTTTACACCCAAGCG‑AACATGTTGGTTTGCAAT‑TGAG‑AAAATACCCATCGTGCTTAACCCAACTCACCCAGGCGATGGGCCCTTTTACggg                                                          >  2:204549/1‑248 (MQ=255)                                                                                                                                                                                           aaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGgaaga                                                       >  1:297834/1‑250 (MQ=255)                                                                                                                                                                                           aaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCCG‑TTTTACCGCAAAGCG‑CATCTGTTGGTTAGCCAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGgaaga                                                       >  1:248471/1‑250 (MQ=255)                                                                                                                                                                                                   gATAGCGACTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCt                                              >  1:94430/1‑251 (MQ=255)                                                                                                                                                                                                    aTAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTg                                             <  1:211475/251‑1 (MQ=255)                                                                                                                                                                                                             aGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgtc                                    <  1:214658/251‑1 (MQ=255)                                                                                                                                                                                                                       tACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGGTAGCAAT‑GGGG‑AAAATAACCCTCGTGATTAAACAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTTGCTTTCGTCGCGGTCTTGt                          >  2:167569/1‑251 (MQ=255)                                                                                                                                                                                                                         cGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGttt                        <  2:217863/251‑1 (MQ=255)                                                                                                                                                                                                                            ttCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTTCCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCAACGTGATTAACCAAACTCAAGCAGGCGGTGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCTATCTTTTTTGcc                     >  1:122187/1‑251 (MQ=255)                                                                                                                                                                                                                            ttCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGACGCCAG‑GTTTACCGCAAAACG‑AAAATATTGGTTAGCAAA‑GGAG‑AAAATACCCCTCGTGATTAAACAAACTCAAGCAGGCGATGGGCCACTTTAAGGAAAAACGAATCATGGCTTTTCTCGCGATCTTGTTTGcc                     >  1:208240/1‑251 (MQ=255)                                                                                                                                                                                                                                 cTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGAAGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTg                 >  1:244544/1‑250 (MQ=255)                                                                                                                                                                                                                                   cccGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGac               <  1:8503/250‑1 (MQ=255)                                                                                                                                                                                                                                   cccGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGac               <  1:262693/250‑1 (MQ=255)                                                                                                                                                                                                                                               gCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCGAACGCACGCAGGCGAGGGGCCATTTTACGGTAGTGCGAAGCCTGGCTTTCGTCGTTATGGTGTTTGCCAGTGACGACTTCTGTAGCa  <  2:202621/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                           GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAT‑GGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCA  >  NC_000913/3387799‑3388287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑