Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,991,238	G→T	intergenic (‑878/‑30)	yqeK ← / → ygeG	protein YqeK/TPR repeat‑containing putative chaperone YgeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,991,238	0	G	T	100.0%	76.8 / NA	23	intergenic (‑878/‑30)	yqeK/ygeG	protein YqeK/TPR repeat‑containing putative chaperone YgeG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/10);  total (13/10)

CATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGC  >  NC_000913/2990995‑2991468                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       cattgcacccatagCAATTGATACTAAGCATCAAGGTCATAAACATTACGGCATTTATGAAATGTCGGAATTCAGATAACTAATACGGCGACTCTTAGTAACACAACTAATGATGTTATTAGTTTGTAGTGACCAGTACTTTTACCAATAATGAAACATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACg                                                                                                                                                                                                                                 >  2:234196/14‑251 (MQ=255)                     aTACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAat                                                                                                                                                                                                              >  1:144427/1‑250 (MQ=255)                          aTGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAAtatta                                                                                                                                                                                                          <  1:315548/249‑1 (MQ=255)                                               tAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAat                                                                                                                                                                                    <  2:46818/250‑1 (MQ=255)                                                       ttATGTCATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaata                                                                                                                                                                            >  1:321913/1‑250 (MQ=255)                                                        tATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa                                                                                                                                                                           <  1:312481/250‑1 (MQ=255)                                                           gTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGt                                                                                                                                                                       <  2:144427/251‑1 (MQ=255)                                                                       ttAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGc                                                                                                                                                            >  2:227945/1‑250 (MQ=255)                                                                                         tGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGc                                                                                                                                          >  1:220190/1‑250 (MQ=255)                                                                                          gAATATTAGTAAAACAACTAATGATGTTATTAGATTGTAGTGAACAGAACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCAt                                                                                                                                        >  1:137782/1‑251 (MQ=255)                                                                                                                       ttaGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGaa                                                                                                                                 <  2:148571/229‑1 (MQ=255)                                                                                                                       ttaGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGaa                                                                                                                                 >  1:148571/1‑229 (MQ=255)                                                                                                                             tGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGt                                                                                                     >  1:212404/1‑251 (MQ=255)                                                                                                                                                      aatGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGaa                                                                            <  2:30148/251‑1 (MQ=255)                                                                                                                                                                                    ttAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGaa                                               >  1:189874/1‑250 (MQ=255)                                                                                                                                                                                    ttAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGaa                                               >  1:12123/1‑250 (MQ=255)                                                                                                                                                                                                  ttAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGACAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAAc                                 <  2:290244/250‑1 (MQ=255)                                                                                                                                                                                                              aaTATAAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAAtt                    <  1:412650/251‑1 (MQ=255)                                                                                                                                                                                                                tataAAAATATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAAttt                   >  1:248538/1‑250 (MQ=255)                                                                                                                                                                                                                   aaaaaTATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATttatta               <  2:347567/251‑1 (MQ=255)                                                                                                                                                                                                                   aaaaaTATCAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAAAttatta               <  2:54976/251‑1 (MQ=255)                                                                                                                                                                                                                           cAATGAGTCAATCAATAATAATTTGTCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCt       >  1:250392/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTCAATCAATAATAATTTGTCATCACGAGACACATCCAAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATAAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGAAATATAACGGACGCAGAAATTTCTTATAAATTCTtgagc  >  1:245761/1‑247 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       CATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAAAACAACTAATGATGTTATTAGTTTGTAGTGAACAGTACTTTTACCAATAATGAAAAATATACCATAAGCAATGCAAGCTTAAAACTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGC  >  NC_000913/2990995‑2991468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑