Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 273,734 G→A Q296Q (CAG→CAA)  ykfC → PF00078 domain‑containing protein YkfC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913273,7340GA97.1% 108.7 / NA 34Q296Q (CAG→CAA) ykfCPF00078 domain‑containing protein YkfC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/17);  minor base C (1/0);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGC  >  NC_000913/273495‑273963
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
gtgCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGTAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCa                                                                                                                                                                                                                             <  1:205256/250‑1 (MQ=255)
    cacaGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCCGGGGAAACCCGCGGTGGCGTCCTGCCCCCTTTCCGCTGATTTTGTTCTCATCGTCCAAGGCACCCAAGCACAAGTGGAAGCCATCAgg                                                                                                                                                                                                                        >  2:148854/1‑251 (MQ=255)
       aGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGggag                                                                                                                                                                                                                     <  2:176943/251‑1 (MQ=255)
               gtTATATCGCCGCTATTCTCGAACATCATGCTGAATGCGATCGATCACTACCTGCATGAGCGCTACCTGCGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGCATAACAGTATCCAACGGGGCCGCAGTACGGCGGTCAGAGAAAACTGGCAGGGGGAAACCGCGGGGGCGTTCCACCGCTATTCCGCTGGTTTTTTCCTCCTCGTCAAAGGCACCCAAGCCCCAGTGGAAGCCCTCaaggggggaggtcc                                                                                                                                                                                                             >  1:167816/1‑238 (MQ=255)
                      cgccgcTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGgtgt                                                                                                                                                                                                       <  2:195432/250‑1 (MQ=255)
                                  cGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGt                                                                                                                                                                                          <  1:370949/251‑1 (MQ=255)
                                      catcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGa                                                                                                                                                                                      <  1:322931/251‑1 (MQ=255)
                                                 aTGAGTTCGATCAATACCTGCATGAGCGCTACCTGCGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAAATGGGAGCGGAAAACCGCGGGGGGGTACTGCCGCTATGACGATGATTTTGTCCTCATCGTCAAAAGCACCCAAGCACACATGGAAGACCTTAGGGGGGAGGGTCGGGGTGTGCCCGCAGGCAGTATGaaaatccggcgg                                                                                                                                                                           >  2:349561/1‑242 (MQ=255)
                                                                    gCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTaa                                                                                                                                                          <  2:3804/249‑1 (MQ=255)
                                                                      aTGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACt                                                                                                                                                                                  <  2:49557/223‑1 (MQ=255)
                                                                      aTGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAACCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGGGGGGTCGAAGGCAGTCTGAAACt                                                                                                                                                                                  >  1:49557/1‑223 (MQ=255)
                                                                           cgcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGGCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCa                                                                                                                                                 <  2:208452/251‑1 (MQ=255)
                                                                                       gggAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACgg                                                                                                                                     <  2:361885/251‑1 (MQ=255)
                                                                                                     aGATCGGTGGTACTGGAATAACAGGATCCAACGGGGCCGAAGTACGGCGGTCAGATAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTGTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCTGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTgg                                                                                                                       <  2:228415/251‑1 (MQ=255)
                                                                                                                aCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGGGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTc                                                                                                            >  1:306586/1‑251 (MQ=255)
                                                                                                                          cAGTATCCAACGGGGCCGAAGTACGGCGGTCAGGGCAAAATGGCAGTGGAAACCCGCGGTGGCGGACTGCCGCAATGCCGATGATTTTGTCCTCATCGGCAAAGGGACCAAAGCACCAGAGGAAGCAATCAGGGAGGGGTGTCGGGGGGTGCGCGAAGGCAGTCTGAAAATCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATTACGGCTTTATCTTTCTTGGGCACCGGCTCAtttgccaa                                                                                                   >  1:329185/1‑244 (MQ=255)
                                                                                                                          cAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGGAAACTGGCAGTGGACAACCGCGGTGGCGTCCTGCCGCTATGCCGATGGTTTTGTCCTCCTCGTCCAAGGCACCCAAGCACAAGTGGGAGCCATCAGGGAGGAGTGTCGGGGGGTGGTCGAAGGCAGTCTTGAACTCAGGCTGAACATGGATAAGACTAACATCCCCCCTGTTAATGACGGCTTTATCTTTTTGGGGcaccagcccattcccaca                                                                                                   >  2:152366/1‑235 (MQ=255)
                                                                                                                          cAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGGCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACGAAAATCCCCCATGTTAATGACGGCGTTAGCTGGCTGGGGCACATGCGCATTCGCAAAc                                                                                                  <  2:167816/251‑1 (MQ=255)
                                                                                                                                                  ggcggTCAGAGAAAACAGGCAGTGGAAACACGCCGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATAGTCCAAGGCACCAAAGCACCAGTGGAAGCCCTCAGGGAGGAGTGTCGGGGAGTGCTCGCAGGCAGTCTGAAACTCCGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTCTGGCGAGATGCg                                                                            >  2:197803/1‑249 (MQ=255)
                                                                                                                                                          gagaAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCa                                                                                                           <  1:124073/210‑1 (MQ=255)
                                                                                                                                                          gagaAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCa                                                                                                           >  2:124073/1‑210 (MQ=255)
                                                                                                                                                            aaaaacggtcattGGAAACCCTCGGTGGCGTACTGCCGCTAGGCCGATGATTTTGTCCTCATCGTCAAAGGCACAAAAGCACAAGTGGAAGCCATCAGGTAGTAGTGTCGGGGTGTGCTCGAAGGCACTCTGAAACTCACGCTGAACATGGATAAGACTAAAATCCCCTATGTTACTGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTGATGTCGCGATGCGAGTGGTCTCAAc                                                                <  2:342888/239‑1 (MQ=255)
                                                                                                                                                               aaCTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGAt                                                             >  1:280457/1‑251 (MQ=255)
                                                                                                                                                                  tGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATccc                                                          >  1:346297/1‑251 (MQ=255)
                                                                                                                                                                                         gTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTcg                                   >  2:168517/1‑251 (MQ=255)
                                                                                                                                                                                          tACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTcgc                                  >  1:89198/1‑251 (MQ=255)
                                                                                                                                                                                           aCTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACAt                                                                                                                                                                      <  1:253704/118‑1 (MQ=255)
                                                                                                                                                                                           aCTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACAt                                                                                                                                                                      >  2:253704/1‑118 (MQ=255)
                                                                                                                                                                                                 gCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCg                           >  1:306553/1‑251 (MQ=255)
                                                                                                                                                                                                   tATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCACCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCt                         >  2:185662/1‑251 (MQ=255)
                                                                                                                                                                                                      gCCGATGATGTTGTCCTCATCGTCAAAGGCACCAAAGCACAAGTGGACGCCATCAGGGAAGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCttac                      <  2:94691/251‑4 (MQ=255)
                                                                                                                                                                                                        cGATGATTTTGTCCTAATCGTCACAGGCACCACAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTCTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGCTAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGGGGTCTCAACGCTCCCCCAGGAGAAAGCCAGACACCTCGCCGCATCGCTGAc                      >  2:213616/1‑249 (MQ=255)
                                                                                                                                                                                                                    ccTCATCGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGa        <  1:148854/251‑1 (MQ=255)
                                                                                                                                                                                                                          cGTCAAAGGCACCAAAGCACAAGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGc  <  2:78471/251‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
GTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGC  >  NC_000913/273495‑273963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑