Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,035	G→A	A246T (GCG→ACG)	metG →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,035	0	G	A	100.0%	78.0 / NA	24	A246T (GCG→ACG)	metG	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/13);  total (11/13)

GGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCT  >  NC_000913/2194837‑2195275                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ggCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATg                                                                                                                                                                                                <  1:37878/249‑1 (MQ=255)       aCGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCt                                                                                                                                                                                          <  1:350436/249‑1 (MQ=255)        cGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTc                                                                                                                                                                                       <  1:336133/251‑1 (MQ=255)               aaTGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATc                                                                                                                                                                                <  1:116970/251‑1 (MQ=255)                                 cttcttcttTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCgcggcg                                                                                                                                                              >  1:17966/1‑251 (MQ=255)                                      tcttTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAg                                                                                                                                                          <  1:80718/250‑1 (MQ=255)                                      tcttTGATCTGCCCTCGTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAAGAAAAGGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTGCAAGAATCTGTGCGACAAGCGCGGCGACa                                                                                                                                                           <  2:338113/249‑1 (MQ=255)                                                             gAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACCTGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGga                                                                                                                                   >  2:142565/1‑250 (MQ=255)                                                                                   gCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATCCTGGAAGAAAGACTCCACCGCCGAGCTg                                                                                                            <  1:310437/251‑1 (MQ=255)                                                                                   gCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTg                                                                                                            >  1:15137/1‑251 (MQ=255)                                                                                      gcggtgcggtGCAGGAGCAGGTGGCGAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTACGCTTCGATGAATACTGGACGAAAGACTCCACCGCCGAGCTGTAc                                                                                                         >  2:307470/1‑251 (MQ=255)                                                                                       cGGTGCGTTGCAGGAGAAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGAAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTAcc                                                                                                        >  2:43406/1‑251 (MQ=255)                                                                                                         ggTGGCAAATACAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGat                                                                                       >  2:171944/1‑250 (MQ=255)                                                                                                                  aaaaaTGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACt                                                                             <  1:6019/250‑1 (MQ=255)                                                                                                                  aaaaaTGCAGGAGTGGTTTGAATCGGGCCTGCAACAGTGGGATATCCCCCGCGACGCCCCTTACTGCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACACGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACt                                                                             <  2:300898/250‑1 (MQ=255)                                                                                                                        gCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCcac                                                                        >  1:168724/1‑250 (MQ=255)                                                                                                                                ggTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTGGGTTTTGAAATCCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATGGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTc                                                                                                   <  2:337195/215‑1 (MQ=255)                                                                                                                                ggTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTc                                                                                                   >  1:337195/1‑215 (MQ=255)                                                                                                                                   ttGAATCTGGATTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGACCACGCCGGGCAAATATTTCTACGTCTGGCTGGACCCACCGATTGACTACATGGGTTCTTTCAAGAATCTGTGCGCCAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTg                                                             <  1:160971/250‑1 (MQ=255)                                                                                                                                           ggCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACAGGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCa                                                     <  1:187082/250‑1 (MQ=255)                                                                                                                                                                            ccTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGgaa                                                                                                                                  >  1:216865/1‑140 (MQ=255)                                                                                                                                                                            ccTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGgaa                                                                                                                                  <  2:216865/140‑1 (MQ=255)                                                                                                                                                                              ttACTTCGGTTTTGCAATTCCGAACACGCCGGGCCAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAAcc                 >  2:57004/1‑251 (MQ=255)                                                                                                                                                                                              aTTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCt  >  1:180576/1‑250 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 GGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCT  >  NC_000913/2194837‑2195275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑