Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	267,149	G→A	intergenic (‑596/+35)	yafY ← / ← yafZ	inner membrane lipoprotein YafY/DUF932 domain‑containing protein YafZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	267,149	0	G	A	100.0%	168.8 / NA	52	intergenic (‑596/+35)	yafY/yafZ	inner membrane lipoprotein YafY/DUF932 domain‑containing protein YafZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/28);  total (24/28)

GGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGG  >  NC_000913/266907‑267394                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ggCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGat                                                                                                                                                                                                                                               >  2:66483/1‑251 (MQ=255)     gATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATAtt                                                                                                                                                                                                                                            <  1:320580/250‑1 (MQ=255)     gATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGGCAGCACGGTGCGATGCGGACAGGTGATGTCCGATAtt                                                                                                                                                                                                                                            >  1:4856/1‑250 (MQ=255)        tATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAAc                                                                                                                                                                                                                                         >  1:337146/1‑250 (MQ=255)               gacagcctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAagcag                                                                                                                                                                                                                                  <  1:37377/250‑1 (MQ=255)                    cctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACAt                                                                                                                                                                                                                            >  1:307516/1‑251 (MQ=255)                                ccGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGGTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTGCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGGCACt                                                                                                                                                                                                                <  2:291825/251‑1 (MQ=255)                                  ggTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGc                                                                                                                                                                                                              >  2:146845/1‑251 (MQ=255)                                     cATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAg                                                                                                                                                                                                            <  1:66483/250‑1 (MQ=255)                                       tGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTg                                                                                                                                                                                                         >  1:310155/1‑251 (MQ=255)                                           ggcgTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTg                                                                                                                                                                                                     <  1:274519/251‑1 (MQ=255)                                            gcgTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGa                                                                                                                                                                                                    <  1:53016/251‑1 (MQ=255)                                              gTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGc                                                                                                                                                                                                  <  2:351573/251‑1 (MQ=255)                                               gtCTGCCTGTTTCATGAGTCGTTATCCGTGAAAGTTAACTGACTGAAAATGATGTGATGACTTGAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTAGTTTTCAGTTCTGCAAAAGCCATAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAAAAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCa                                                                                                                                                                                                 <  2:340588/250‑1 (MQ=255)                                                               agaCGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCAc                                                                                                                                                                                 >  1:96627/1‑251 (MQ=255)                                                                   gTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACATGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCcac                                                                                                                                                                             >  1:290510/1‑251 (MQ=255)                                                                        ccGTGAAAGTTAACTGACTAAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAg                                                                                                                                                                           <  2:227001/248‑1 (MQ=255)                                                                                gTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTaa                                                                                                                                                                <  1:280061/251‑1 (MQ=255)                                                                                            aaaaTGATGTGATTACTTTAAAGAGGTGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTccc                                                                                                                                                    <  2:358955/251‑1 (MQ=255)                                                                                                       aTTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACcg                                                                                                                                         >  1:12377/1‑251 (MQ=255)                                                                                                          aCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACcgcg                                                                                                                                       >  2:10445/1‑250 (MQ=255)                                                                                                              tAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACg                                                                                                                                   <  1:146845/250‑1 (MQ=255)                                                                                                                aagagtgatgcggtcAGGGAATGGACACCAAATAAGATATTTGTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCACGGTGAGTGAGGACCTTCAGCATTGTGCGATGGGGACAGGTGATGTCCGATATTAACAAATCAGCATCATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTGCCGCCATCACCCACATTGCGCGGTTCAGTGTCAAGTCTCTGTCTATACCGCCAACgtca                                                                                                                                <  2:177575/237‑4 (MQ=255)                                                                                                                aaGAGGGGGTCGGACAGGGTATGGACACCCCAGACACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGAGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCAAGTCCCCGTCGATACCGCGAACGGcc                                                                                                                                >  2:384471/1‑250 (MQ=255)                                                                                                                   agGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACGGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACgg                                                                                                                             <  1:170522/251‑1 (MQ=255)                                                                                                                   agGGGGCCGGACCGGGTATGGACACCACAGAAACTATTTTTCATTTCTGAAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGcacggcacgg                                                                                                                             >  2:394938/1‑251 (MQ=255)                                                                                                                      gggTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGt                                                                                                                           >  1:318254/1‑250 (MQ=255)                                                                                                                                   tATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGtcctc                                                                                                             >  1:124002/1‑251 (MQ=255)                                                                                                                                      ggACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCCCAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCtt                                                                                                          <  2:309817/251‑1 (MQ=255)                                                                                                                                                 aaaCTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTcccc                                                                                                                                                   >  2:153981/1‑199 (MQ=255)                                                                                                                                                 aaaCTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTcccc                                                                                                                                                   <  1:153981/199‑1 (MQ=255)                                                                                                                                                   aCTATTTTTCATTTCTGCAAAAGCCCGAGGCAGCGGGGGTTGCAGGCCTTCCCGCGGGGAGTGAGTACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGGCGCTGCAGTTGCGTGAGCAGGTCGTCCGCCATCTCCCTCAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTTGCATTACGGcc                                                                                             <  2:351148/251‑1 (MQ=255)                                                                                                                                                   aCTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGcc                                                                                             <  2:120341/251‑1 (MQ=255)                                                                                                                                                    cTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGcc                                                                                             >  1:163542/1‑250 (MQ=255)                                                                                                                                                                              agGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTTACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTACTCAGGTtctc                                                                  >  2:147949/1‑251 (MQ=255)                                                                                                                                                                               ggCAGCGGGGGTTACATGCCTTCACTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTcc                                                                 <  2:290510/251‑1 (MQ=255)                                                                                                                                                                                gCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCt                                                                <  2:325846/251‑1 (MQ=255)                                                                                                                                                                                     gggggTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGTCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAAt                                                            >  1:260535/1‑250 (MQ=255)                                                                                                                                                                                     gggggTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATa                                                           <  1:146352/251‑1 (MQ=255)                                                                                                                                                                                      ggggTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGCTACCGCAAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATAc                                                          >  2:117716/1‑251 (MQ=255)                                                                                                                                                                                        ggTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGc                                                        >  1:128621/1‑251 (MQ=255)                                                                                                                                                                                           tACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTGGGCATTACGGCCACGGAGCCCGCCCTGAATCAGGTTCTCCTGAATACGCTg                                                      <  2:202854/250‑1 (MQ=255)                                                                                                                                                                                                   ttCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGt                                             >  1:93714/1‑251 (MQ=255)                                                                                                                                                                                                          ggTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAgg                                      <  2:337146/251‑1 (MQ=255)                                                                                                                                                                                                             gagtgagGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTc                                    <  1:26614/250‑1 (MQ=255)                                                                                                                                                                                                              agtgagGACCGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCAt                                  <  2:307516/251‑1 (MQ=255)                                                                                                                                                                                                                       cGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCAc                                                                                                                               <  1:101013/149‑1 (MQ=255)                                                                                                                                                                                                                       cGTCAGCACGGTGCGATGCGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCAc                                                                                                                               >  2:101013/1‑149 (MQ=255)                                                                                                                                                                                                                            gCACGTTGCGATGCCGACAGGTGAGGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCTCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACACTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGc                    <  2:405614/251‑1 (MQ=255)                                                                                                                                                                                                                               cGGTGCAATGCGGACAGTTAATGTCAGCTATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGCCTTCCGCCATCACCAACAGTGCCCGGTTAAGTTTCACGTCACCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGACTACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGcc                   >  2:267432/1‑249 (MQ=255)                                                                                                                                                                                                                                         cGGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAgggg       >  2:405989/1‑251 (MQ=255)                                                                                                                                                                                                                                              aGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTTCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAgg  <  1:390594/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGG  >  NC_000913/266907‑267394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑