Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,367	C→A	T119N (ACC→AAC)	prfA →	peptide chain release factor RF1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,367	0	C	A	92.9%	81.1 / NA	29	T119N (ACC→AAC)	prfA	peptide chain release factor RF1
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base A (12/14);  minor base G (0/2);  total (12/17)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATA  >  NC_000913/1265127‑1265603                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              cACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGcgac                                                                                                                                                                                                                                     <  2:237437/251‑1 (MQ=255)      gAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAgc                                                                                                                                                                                                                                >  2:129672/1‑251 (MQ=255)       aaTATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAAGATGAACTGCGCGAAGCTGAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAgcg                                                                                                                                                                                                                               <  1:258387/251‑1 (MQ=255)            gcgcAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCt                                                                                                                                                                                                                          <  1:129672/251‑1 (MQ=255)                               cgcgCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTc                                                                                                                                                                                                       >  1:76167/1‑251 (MQ=255)                                gcgcTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTcc                                                                                                                                                                                                      <  2:384978/251‑1 (MQ=255)                                             gACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCg                                                                                                                                                                                         <  1:293270/251‑1 (MQ=255)                                                    aaCAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGcc                                                                                                                                                                                  >  1:8122/1‑251 (MQ=255)                                                           tCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCTCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGcc                                                                                                                                                                            <  2:250413/250‑1 (MQ=255)                                                                  gATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTg                                                                                                                                                                    >  2:302353/1‑251 (MQ=255)                                                                              gCACAGATGATGCTCGATGATCCTGAAATGCGTGCGATGGCGCCGGATGACCTGCGCGAAGCTAAAGACAAACGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAACAGATCCTGATGACGCACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGCAGCGGCGCTGTTCCCGGGCGATCTGTTCCGTCTGTACAGCCGTTCTGCCGACGCCCGCCGCTTGCGGGtttaacc                                                                                                                                                        >  2:94643/1‑245 (MQ=255)                                                                                                 atCCTGAAATGCGGGAGATGGCGCGGAATGAACGGCGAGAAACTAAAAAAAAAAGCGAGCAACGGGAACAGCAATTACAGGTTCTGTGACGGC‑CAAAAGATCCTGAGGCCGAACGAAACGCCTTCCTCGAAGTCCGAGCCGGAAGCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCGGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAggg                                                                                                                                     <  2:292794/250‑1 (MQ=255)                                                                                                      gAAATGCGTGAGAGGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATGACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAGGCCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACa                                                                                                                               <  1:302353/251‑1 (MQ=255)                                                                                                         aTGCGTGAGATGGCGCCGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCccgcc                                                                                                                                                                        >  2:206277/1‑208 (MQ=255)                                                                                                         aTGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCccgcc                                                                                                                                                                        <  1:206277/208‑1 (MQ=255)                                                                                                         aTGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGGGAACAtgg                                                                                                                            >  1:42653/1‑251 (MQ=255)                                                                                                                  aTGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCCGCGAGGGGGAACATGGGGGGtata                                                                                                                    >  1:21422/1‑250 (MQ=255)                                                                                                                     gcggaggaggAACTGCGCGAAGCAAAAGAAAAAAGCGAGAAACTGAAAAAGAAATTACAGGTTCTGTTACTGC‑AAAAAGATCCTGATGACGAACGTAACGCCTCCCTCGAAGTCCGGGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAaga                                                                                                                <  2:21422/247‑1 (MQ=255)                                                                                                                      cgcAGGATGAACTGCGCGAAGCTAAAGAAAAA‑GCGAACCACTGGAACCGAACTTAAAGGCTCTGGTACTGA‑CAAAAAATCCTGATGCCGAACGTACCGGCTTCCTCGAAGTCCGAGCCGGAAAAGGCCGCGCAGAAGCGGCGCCGTTCGCGGGCGATCTGTTCCGTATGGACAGCCGTTATGCCGAAGACCGCCGCTGGCGGGGAGAAATAATGGGCGGCCAGCGAGGG‑GGACAtggtgggtttaaaca                                                                                                                >  1:259669/1‑240 (MQ=255)                                                                                                                                                           gCAACTGGAAAAGAAATTACGGGTTTTGTTACGGC‑CAAAAGACCCTGAGGACGACCGAAACGCCTGCCTCGAAGTCCGAGCAGGAAAGGGGGGCGACGAGGGGGCGCTGTTCGGGGGCAATCTGTTCCGTATGTACGGCCGTTATGCCGAGGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGgtc                                                                          <  2:73714/251‑1 (MQ=255)                                                                                                                                                             aaCTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGACCATGGGGGTTTTAAAGAGATCATCGCCCACATTATCGGTGATTGTGTGTATGgtcgt                                                                        >  1:148149/1‑251 (MQ=255)                                                                                                                                                                         aaTTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCG‑CCAGCGAGGGTGGACATGGGGGGTTTAAAGAGATCATCGCCCAAAATAGCGGGGGTGGGGTGTATGGTTGTCTGAAATTTgt                                                             >  2:113634/1‑249 (MQ=255)                                                                                                                                                                          aTTACAGGTTCTGTTAC‑GCACAAAAGACCCTGATGACGAACGTACCGCCTCCCTCGAAGTCCGAGCCGGAAACGGGGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAAt                                                           <  2:84785/251‑1 (MQ=255)                                                                                                                                                                            accAGGTTGTGTTACTGC‑AAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGCCGGAGCGGGAAACGGGGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTCCCGAATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATcc                                                         <  1:150924/249‑1 (MQ=255)                                                                                                                                                                               aGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGGGGACATGGGGGTTATAAAGAGAACATCGCCAAAATTAGCGGTGGTGGGGTGGATGGTCGTCTGAAATTTTAATAcggg                                                      >  2:219563/1‑250 (MQ=255)                                                                                                                                                                                ggTTTTGTTACGGC‑AAAAAGACCCTGATGAGGACCGTAACGCCTTCCTCGAAGGCCGAGCCGAAAGCGGGGGCGAGGAGGCGGGTCTTTTCGCGGGCGATCTGTTCGGTAGGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcggcg                                                     <  2:25510/251‑1 (MQ=255)                                                                                                                                                                                         aCTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATcgc                                             <  1:113634/250‑1 (MQ=255)                                                                                                                                                                                                                 aaCGTAACGCCTTCCTCGAAGTCCGAGCCGGAAACGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGaa                     <  1:163813/251‑1 (MQ=255)                                                                                                                                                                                                                                    gctccgacctgaaaCCGGCGGGGACGAAGCGGCGGTGTTGGGGGCCGATCTTTGTCGTATGGACAGCGGTTATGCCGACGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGGGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCAGCGCGTGCAACGTGTTCCTGCTACGGAATCTCAGGGTCGTATTCATa  <  2:385949/239‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              CACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGC‑CAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCG‑CCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCGCAGGGTCGTATTCATA  >  NC_000913/1265127‑1265603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑