Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	498,522	Δ406 bp	coding (468‑873/963 nt)	hemH →	ferrochelatase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	498522	498927	406	36 [0]	[0] 36	hemH	ferrochelatase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 498521	0 (0.000)	36 (0.890)	34/482	0.3	100%	coding (467/963 nt)	hemH	ferrochelatase
	?	NC_000913	498928 =	0 (0.000)					coding (874/963 nt)	hemH	ferrochelatase

GTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGATGATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGAC  >  NC_000913/498683‑499168                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ccgctgatggtttacagccgccagcaacagcaggcgctggcacaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCggg                                                                                                                                                                                                                                             >  2:157525‑M2/246‑251 (MQ=255)      gatggtttacagccgccagcaacagcaggcgctggcacaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGaaaaa                                                                                                                                                                                                                                        <  1:104963‑M2/11‑1 (MQ=255)           tttacagccgccagcaacagcaggcgctggcacaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagccttccggggatGGCGGGAAaaaatgtg                                                                                                                                                                                                                                   >  2:212626‑M2/236‑248 (MQ=255)                           acagcaggcgctggcacaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCt                                                                                                                                                                                                                   <  1:157525‑M2/32‑1 (MQ=255)                             agcaggcgctggcacaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCtt                                                                                                                                                                                                                  >  1:270090‑M2/218‑250 (MQ=255)                               caggcgctggcacaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAAt                                                                                                                                                                                                               >  1:383091‑M2/216‑251 (MQ=255)                                       ggcccaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggtgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGcc                                                                                                                                                                                                       <  1:217154‑M2/44‑1 (MQ=255)                                          acaacgtttaccggagatgcccgtcgcgctgggcatgagctacggctcgccatccctggaccgcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggattaactggcacgcattctggcgcgcacacgtagcattccggggatGGCGGGCAAAAATATGCATATATTCCGGCGCt                                                                                                                                                                                                                   >  2:192101‑M2/205‑236 (MQ=255)                                          acaacgtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggggctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCt                                                                                                                                                                                                                   <  1:192101‑M2/32‑1 (MQ=255)                                               gtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcccgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATCTTCCTGCGCTTAATGCCACGCCGGAACat                                                                                                                                                                                                >  2:213151‑M2/200‑250 (MQ=255)                                               gtttaccggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctagcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGTAAAAATCTGAATATATTCCGGCGCTTAATGCCACGCCGGACCat                                                                                                                                                                                                >  2:213155‑M2/200‑250 (MQ=255)                                                   accggagatgcccgtagcgctgggaatgagctacggctcgccatcactggaaatcgccgtagatgaactcctggaatagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCg                                                                                                                                                                                            <  1:379802‑M2/55‑1 (MQ=255)                                                       gagatgcccgtagcgctgggaaggagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATTTGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatg                                                                                                                                                                                       <  2:391635‑M2/60‑1 (MQ=255)                                                          atgcccgtagcgctgggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatgatg                                                                                                                                                                                    >  1:358290‑M2/189‑251 (MQ=255)                                                                        gggaatgagctacggctcgccatcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGc                                                                                                                                                                      >  2:411734‑M2/175‑251 (MQ=255)                                                                                            catcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTg                                                                                                                                                    >  1:134097‑M2/155‑249 (MQ=255)                                                                                             atcactggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGTGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGc                                                                                                                                                 <  1:262090‑M2/98‑1 (MQ=255)                                                                                                 ctggaaagcgccgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGt                                                                                                                                              >  2:207969‑M2/150‑250 (MQ=255)                                                                                                            cgtagatgaactcctggcagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAgc                                                                                                                                  >  2:230554‑M2/139‑251 (MQ=255)                                                                                                                             cagagcatgtagatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCt                                                                                                                 >  1:121697‑M2/122‑251 (MQ=255)                                                                                                                                   atgtagatcatagtggtgtgctgccgctttatccgcaattctcctgttctacggtcggttcggtatgggatgaactggcacgcattcttgcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATGCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTGGTTGCGGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                                           <  1:251218‑M2/136‑1 (MQ=255)                                                                                                                                       agatcatattgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgccaacgtagcattccggggatGGCGGGCAAAAATATGAATATATTCCGGCGCTTACTGCCACGCCGGAACATATCGACATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGCCGCGAAGAGCCTCGtcggcggg                                                                                                       >  2:299055‑M2/112‑250 (MQ=255)                                                                                                                                                tgtggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTcatcat                                                                                              >  1:383620‑M2/103‑251 (MQ=255)                                                                                                                                                  tggtgctgccgctttatccgcaattatcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGACAAAATATGCATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGCCGCGAAGAGCCTCGTCGGCGGTTTTCATCATcc                                                                                            >  2:347595‑M2/101‑251 (MQ=255)                                                                                                                                                   ggtgctgccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCg                                                                                           <  2:274148‑M2/152‑1 (MQ=255)                                                                                                                                                         gccgctttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGCTTTCATCATCCGTGaat                                                                                      >  1:158763‑M2/94‑250 (MQ=255)                                                                                                                                                               ttatccgcaattctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGc                                                                                 <  1:86666‑M2/162‑1 (MQ=255)                                                                                                                                                                        attctcctgttctacggtcggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTg                                                                      <  2:383620‑M2/173‑1 (MQ=255)                                                                                                                                                                                          cggtgcggtatgggatgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTagag                                                    <  1:394566‑M2/191‑1 (MQ=255)                                                                                                                                                                                                        atgaactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAgg                                      <  2:13316‑M2/205‑1 (MQ=255)                                                                                                                                                                                                           aactggcacgcattctggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGCATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGGGCCTGGGGAGAGTTTGAACTCACAGggcc                                   >  2:281766‑M2/44‑250 (MQ=255)                                                                                                                                                                                                           aacgggcacgcattcttgcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATGCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATTCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGgct                                   <  1:37752‑M2/208‑1 (MQ=255)                                                                                                                                                                                                                           ggcgcgcaaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCt                     <  1:411734‑M2/222‑1 (MQ=255)                                                                                                                                                                                                                                 caaacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTg             <  2:361980‑M2/230‑1 (MQ=255)                                                                                                                                                                                                                                   aacgtagcattccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGt           <  2:221609‑M2/232‑1 (MQ=255)                                                                                                                                                                                                                                             tccggggatGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGAc  <  2:383091‑M2/241‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGATGATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGAC  >  NC_000913/498683‑499168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑