Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,282,3321.A96.0% 85.6 / ‑4.2 25coding (744/1293 nt)yjcFpentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (12/12);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCATATTAGAGTCGGTACATATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAAC  >  NC_000913/4282088‑4282564
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
ccATATTAGAGTCGGTACATATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑aaaaaatttg                                                                                                                                                                                                                                      >  1:325701/1‑245 (MQ=255)
ccATATTAGAGTCGGTACATATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGa                                                                                                                                                                                                                                     <  1:396166/251‑1 (MQ=255)
                            ttCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATAtt                                                                                                                                                                                                          <  1:221024/250‑1 (MQ=255)
                              cGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATGAAAAA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGc                                                                                                                                                                                                       >  2:111720/1‑251 (MQ=255)
                               gAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGct                                                                                                                                                                                                      <  2:328836/251‑1 (MQ=255)
                                            gTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCAttgttg                                                                                                                                                                                         >  2:164143/1‑251 (MQ=255)
                                                 ccGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAATGTATTTTTAATATTGGCTCTATTCATTGTTGCATTg                                                                                                                                                                                    <  1:376531/251‑1 (MQ=255)
                                                    cgTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGt                                                                                                                                                                                   >  2:107519/1‑249 (MQ=255)
                                                          aaaGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTg                                                                                                                                                                            >  1:316253/1‑250 (MQ=255)
                                                                               gTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGaa                                                                                                                                                      <  1:203745/251‑1 (MQ=255)
                                                                                       tCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTagag                                                                                                                                               <  2:74927/250‑1 (MQ=255)
                                                                                            aGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGAGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCAGAT‑AAAAAATTTGAATAATCAAGGAAAGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCgt                                                                                                                                         <  2:108337/251‑1 (MQ=255)
                                                                                                        ttCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCAttttt                                                                                                                              >  2:112656/1‑250 (MQ=255)
                                                                                                                gACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCg                                                                                                                     <  1:123120/251‑1 (MQ=255)
                                                                                                                          aGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGt                                                                                                           >  1:155875/1‑251 (MQ=255)
                                                                                                                              cAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATc                                                                                                       <  1:65072/251‑1 (MQ=255)
                                                                                                                                gCATGTTCAAAGTTAATTAAATCAAGTTCAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAACGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTCTTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGCGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGATTCATCg                                                                                                      >  2:326430/1‑250 (MQ=255)
                                                                                                                                  aTGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCg                                                                                                      >  2:236854/1‑248 (MQ=255)
                                                                                                                                                  aaaTCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTg                                                                                                                                                                                                         >  1:282973/1‑133 (MQ=255)
                                                                                                                                                  aaaTCAAGATTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTg                                                                                                                                                                                                         <  2:282973/133‑1 (MQ=255)
                                                                                                                                                            tAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTAtgtttt                                                                          <  1:52457/250‑1 (MQ=255)
                                                                                                                                                                                  aaataaatTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTaaaa                                                      <  1:217026/248‑1 (MQ=255)
                                                                                                                                                                                                  ggCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTAc                                   >  1:157349/1‑251 (MQ=255)
                                                                                                                                                                                                                         tACCTCAGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGt            >  2:238023/1‑251 (MQ=255)
                                                                                                                                                                                                                               aGCCATGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTg      >  1:281486/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aTGTATGCCATAT‑AAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAAc  >  1:294517/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
CCATATTAGAGTCGGTACATATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATAT‑AAAAA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAAC  >  NC_000913/4282088‑4282564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑