Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	165.1 / NA	44	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (21/23);  total (21/23)

TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCT  >  NC_000913/3560209‑3560697                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   ttttttCCCATCAAGGTTTTAGCAAGGTGGAGATTGTTG‑TGGAAATGGCGAAATTTGTTGGGATTGAGCAGTGGGTGCGCTCCCTCTGCCGGGGGGGGGCGGATATCGAAAAACAGCGTCGAGCCATTGGGGATTGGCCCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcg                                                                                                                                                                                                                                                    <  2:386543/246‑2 (MQ=255)     ttACCATCAACGTGTTAGCAACGTTGTGATTGTTGGTGACAATG‑CGCAAATTGCGTTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGGGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAt                                                                                                                                                                                                                                              <  1:93927/250‑1 (MQ=255)         caacAACGGGTTAGAAACGTTGAGAGTGTTGGGAAAAATG‑CAAAAATTGGTGTGTTGGAGCGGGGGGGGCGCTACCGCTTCCGGGGGGGGGCCGAAATCGAAAAACAGCGCCGAGCCTTTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGt                                                                                                                                                                                                                                         <  2:402602/248‑1 (MQ=255)           tCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGAGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGAGCCGCCCCGCCATGATGGCGCAGGATCAGGttt                                                                                                                                                                                                                                       >  2:276864/1‑251 (MQ=255)            aagcgggtgagaaaCGTTGAGTTTTTTGGTGACAAGG‑CGAAAATTGCTGTGATGGAGCAGTGCGTGCGCTCCCGCTTCCGGGGTGGTGCCGATATCGATAACCGGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtttt                                                                                                                                                                                                                                      <  2:270003/239‑1 (MQ=255)             aaCGTGTTAGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                     >  2:75437/1‑251 (MQ=255)                gtgtTAGAAAGGTTGAGTGTGGTGGTGAAAATG‑CGAAAATTGGTGTGATTGGGCAGTGCGTGCGCTCCCGCTTCGGGCGGGGTGCCGATAGCGAAAAACGGCGTCGGGCCATTGGTGATTTGCCCCG‑CCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGTCGCAGGATCAGGTTTTGCTc                                                                                                                                                                                                                                  <  2:350309/251‑1 (MQ=255)                                 aTTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCTTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGt                                                                                                                                                                                                                 >  2:33669/1‑251 (MQ=255)                                                   gcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                                >  1:330705/1‑251 (MQ=255)                                                    cAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGACTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                                               >  1:404962/1‑251 (MQ=255)                                                                                  tACCGCTTCCGGCGTGGTGCCGATATCGATCAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                 >  2:171666/1‑251 (MQ=255)                                                                                   aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                                >  1:334811/1‑251 (MQ=255)                                                                                      gCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                             >  2:126634/1‑251 (MQ=255)                                                                                       cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                                               >  2:161577/1‑216 (MQ=255)                                                                                       cTTCCGGCGGGGTGCCGATATCGATAAACAGCGGCGGGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                                               <  1:161577/216‑1 (MQ=255)                                                                                              cGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCAGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                                                                                                                                     >  1:158627/1‑251 (MQ=255)                                                                                                gggtgCCGATATCGATAAACAGCGTCGGGCCTTTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa                                                                                                                                                   <  1:239813/250‑1 (MQ=255)                                                                                                   tgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                                >  1:130927/1‑251 (MQ=255)                                                                                                            cGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                       <  1:58542/251‑1 (MQ=255)                                                                                                            cGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCGCCAAGATGGCGCAGGATAAGGATTTGCTCCCCCAGCGCATTGTGGTCGCGGAGAGTAGTCTGCGGGTGGACGGAGAAATTCTCTACAAGCTCGTCTGTACTGACATAACCCTGCCGttt                                                                                                                                        >  2:64261/1‑250 (MQ=255)                                                                                                                aaaacgggTCGAGGCCTTGGGGGTTTGGTCCGGCCACTTTGGGGGCGATGCGCTTTTTTTCTCCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                    <  2:308200/244‑1 (MQ=255)                                                                                                                   cggCGTCGAGCCTTTGGGGATTTGCTCCG‑CCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                                                <  1:288007/249‑1 (MQ=255)                                                                                                                           aGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTAGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                         >  1:402861/1‑250 (MQ=255)                                                                                                                             ccATGGGGGATTTGCTCCG‑CCACTTTGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc                                                                                                                      <  1:26066/251‑1 (MQ=255)                                                                                                                                  ggggATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGCCTGCGGGCTGTCGGATAAATGCTCTACCAGCTCTTCGTTAATGACATTACCTTGCTGTTTAACCattcccaaaatacccttgt                                                                                                                 >  2:87947/1‑233 (MQ=255)                                                                                                                                         tGCTCCG‑CCACTTGGCGGGCGATGCGCTCTTTTTCTTGGGTCTGGGTGG‑CCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                           <  1:362225/250‑1 (MQ=255)                                                                                                                                             ccG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGCTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGAGACGTTGtgtt                                                                                                      >  2:12064/1‑251 (MQ=255)                                                                                                                                              cccccACTTTGGGGGCGATGCGTTCTTTTTCTTCGGTCTGGGGGG‑CCTTGCGATCGTGCCAGGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg                                                                                                        <  2:269749/246‑1 (MQ=255)                                                                                                                                                        gcggggggtgcgcttgttttCTTGGGTCTGGGGGG‑CCTTGCGGTCGTGCCAGGGCGTTGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTGGTGTTTGTTTCAttt                                                                                            <  2:153860/235‑1 (MQ=255)                                                                                                                                                            gggATGCGCTCTTTTTCTTCGGTCGGGGTGG‑CCTTGCGCTCGGGCCACGGGGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                                        <  2:346182/249‑1 (MQ=255)                                                                                                                                                            gcggtGCGTTCTTTTTCTCCGGTC‑GGGTGGGCGTGGCGTTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTGGTGACGTTGTGTTTGTTTCATTTATaa                                                                                        <  2:378838/247‑1 (MQ=255)                                                                                                                                                               aTGCGCTCTTTTTCTTCGGTCGGGGTGG‑CTTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                                                     <  1:77058/251‑1 (MQ=255)                                                                                                                                                                       tttttCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGaa                                                                              <  1:38712/250‑1 (MQ=255)                                                                                                                                                                               cGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcg                                                                     <  1:304727/251‑1 (MQ=255)                                                                                                                                                                                      ggTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgc                                                              >  1:186444/1‑251 (MQ=255)                                                                                                                                                                                       gTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGGTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgcg                                                             <  1:337855/251‑1 (MQ=255)                                                                                                                                                                                                    tCGTGCCACGGAGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGCACTGACATAACCCTGCTGTTTAACCAGCTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATACATCACTGGCATTATTTTCGCTTTCGCGCATTGAGCGaac                                                 >  2:341039/1‑250 (MQ=255)                                                                                                                                                                                                      gTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca                                               <  2:141031/251‑1 (MQ=255)                                                                                                                                                                                                       tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca                                               >  1:209034/1‑250 (MQ=255)                                                                                                                                                                                                                                   gCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTaa                  <  1:293102/251‑1 (MQ=255)                                                                                                                                                                                                                                    cAGCGCCCCACCGACATGATGGCGCAGGCTCAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGGCGAATAGTCTGCAGGCTGACGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTATCCAGTTCGATAATACCGTTGTGACGTTGTGTCTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAACAACAACAGCCATCGCTAAACCCACGGcgacc                 >  2:212504/1‑247 (MQ=255)                                                                                                                                                                                                                                             aCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtg        >  2:380466/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                                        >  1:294625/1‑168 (MQ=255)                                                                                                                                                                                                                                                gCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                                        <  2:294625/168‑1 (MQ=255)                                                                                                                                                                                                                                                   atgatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCt  <  2:346098/251‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCG‑CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCT  >  NC_000913/3560209‑3560697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑