Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,375,828	IS186 (+) +4 bp	coding (132‑135/963 nt)	menC ←	o‑succinylbenzoate synthase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2375828 =	0 (0.000)	25 (1.010)	24/484	0.2	100%	coding (135/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	= 2515617	NA (NA)					noncoding (1345/1345 nt)	IS186	repeat region
*	?	NC_000913	= 2375831	1 (0.040)	18 (0.730)	18/484	0.5	94.7%	coding (132/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	2514273 =	NA (NA)					noncoding (1/1345 nt)	IS186	repeat region

GCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCAT  >  NC_000913/2514025‑2514517                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     gccgAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTGCCTGGTTGCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCCGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAATCCGGGGATTATTcc                                                                                                                                                                                                                                                    <  2:129423/248‑1 (MQ=255)     aaaaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATa                                                                                                                                                                                                                                                <  1:188176/251‑1 (MQ=255)                 aCGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACtt                                                                                                                                                                                                                                     >  1:97419/1‑250 (MQ=255)                   cctctctCCCCGTGCTGAAGGCATCATCTCTGTCTACCTGGTTTCCTCCCCTAACTTCTCTTCAATCGGGTTTAACGCAGGTGCAGTTAAAGACCTGAAGGAAGAGCAAGGTAACGTGGTTGCTCGCTGCCGTCTTAAGCTTGTTTAAGGCTCTACCCTGGCGAGTGTGCTTTCTGCGTCAATCGCAGCAATGGTGCTGTAAGACCATACATAAAAAAGCCGTGTATAATTCCCATAAGCCCTAACTTaa                                                                                                                                                                                                                                   >  2:115795/2‑250 (MQ=255)                             cgTGCTTAAGGCATCATCTCTGTGTTGCTTGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGGTTACGGCTCTACCCTGGTGATTGTGCTGTCTGCGTCAATCGTAGCACTGGTGCTGTAAGAACATACATAAAAAAGCCGGTTATAAGTCCCATAAGCGCTAACTTAAGGGTTGTTGTa                                                                                                                                                                                                                        <  1:141850/251‑1 (MQ=255)                                         atcatcTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                                                                                                                                                                                                            <  1:211648/251‑1 (MQ=255)                                          tcatcTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTCACTTAAGGGTTGTGGTATTACGCCTGatat                                                                                                                                                                                                           >  2:216843/1‑251 (MQ=255)                                                  gtgtTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTTCGTCAATCTCAGCACTGGTGCTGGAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCGGATATGATTTa                                                                                                                                                                                                     <  2:157210/249‑1 (MQ=255)                                                       ccTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGcc                                                                                                                                                                                              >  1:113435/1‑251 (MQ=255)                                                               ccTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGGTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGTGGATAATTCCCATAAGCGCGAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                                                                                                                                                                                       <  1:167520/250‑1 (MQ=255)                                                                  tCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCTGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAc                                                                                                                                                                                    <  2:120450/250‑1 (MQ=255)                                                                   cGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActc                                                                                                                                                                                  <  1:158547/251‑1 (MQ=255)                                                                     cTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTTAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGatat                                                                                                                                                                                                           >  1:100560/1‑224 (MQ=255)                                                                     cTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTTAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTGAAGGGTTGTGGTATTACGCCTGatat                                                                                                                                                                                                           <  2:100560/224‑1 (MQ=255)                                                                                tCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTg                                                                                                                                                                      <  2:87182/250‑1 (MQ=255)                                                                                      gggATTATCGCAGGTGCAGTTAAAGGCCTGAATGCAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGc                                                                                                                                                                >  1:227934/1‑250 (MQ=255)                                                                                        gATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                                                                                                                                                                                                        >  1:189545/1‑192 (MQ=255)                                                                                        gATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                                                                                                                                                                                                        <  2:189545/192‑1 (MQ=255)                                                                                             tCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg                                                                                                                                                        >  1:92287/1‑251 (MQ=255)                                                                                                    tGCAGTTAAAGGCCTGAAAGTCGATCCAGGTAACGTGGTTTCTCGCTTCGGTCTTATGCTGGTTTACGGCTCTACCCTGGGGAGTGTGTTGTCTGCGTCAATCGCAGCACTGGTGCTGGAAGACCGTACATAAAAAATCGGGTGATAATTGCCATAAGGGCTGACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGTCGATGAATTACTCTCACGATAACTGGTCAGTAATTCTGGCCCacat                                                                                                                                                 >  2:51987/1‑248 (MQ=21)                                                                                                           aaaGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAg                                                                                                                                          <  2:134233/251‑1 (MQ=255)                                                                                                            aaGGCCTGAATGCAGAGCAAGGTAACGTGTTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTATACCCTGGTGAGTGGGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATACAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGATGTGGGATTACGCCTGATATGATTTAACGTGCCGAAGAATTACTCTCACGATAACTGGCCATCAATTCGGGACCATATTGGTAAGc                                                                                                                                         >  2:221326/1‑251 (MQ=255)                                                                                                                  agaatgaaGAGCAAGGGGACGTGGTTTCCCGCTTCGGGCTGAAGCTGGTCTACGGCTTTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGCGAGGCCATACGTAAAAGAGCCGGGGATAATTCTCATAAGCGCTAACTTAAGAGGTGTGGTATTACGCCTGATCTGATTTAACGTGCCGATGAATTATTCTCAAGATAACTGGTCAGAAATTCTGGCACATATTGGAAAGCCCgaat                                                                                                                                   >  2:9509/2‑250 (MQ=255)                                                                                                                   gaatgaaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCga                                                                                                                                     >  1:169261/1‑248 (MQ=255)                                                                                                                             cAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGTTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACt                                                                                                                        >  1:167204/1‑251 (MQ=255)                                                                                                                                               cGCTTCGGTCTTAAGCTTGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCCGGGATATTTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATAGGATTTAACGTGCCGATGAATTACTCGCACGAAAACTGGTCATGAATGCTGTCCCATATTGGTAAGCCCGAAGAGCTGGGTAccaccggaagtaaggcc                                                                                                         <  2:26745/248‑17 (MQ=21)                                                                                                                                               cGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGcc                                                                                                         <  2:219021/248‑1 (MQ=255)                                                                                                                                                    cGGTCTGAAGCTGGTTTAAGGCTCTGCCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCGGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTGAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCAATATTGGTAAGCCCGAAGAACTGGAGACTTCGGCACGTAATGCCGGGGccc                                                                                                  <  2:238224/250‑3 (MQ=21)                                                                                                                                                         tGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGACGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAAccc                                                                                            >  2:178801/1‑251 (MQ=255)                                                                                                                                                                ggTTTACGGCTCTGCCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAATGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTTGCCCATATTGGTAAGCCCGAAGAACTGGATTCTTGGGCACGTAATGCCGGGGCTCTAACccgccgcc                                                                                      <  1:145232/250‑1 (MQ=255)                                                                                                                                                                           ctACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCg                                                                            <  1:59860/249‑1 (MQ=255)                                                                                                                                                                                                cTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTc                                                     >  2:15565/1‑251 (MQ=255)                                                                                                                                                                                                   cGTCAATCGCAGCTCTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTGAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTgg                                                  <  2:195364/251‑1 (MQ=255)                                                                                                                                                                                                                            aGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTTCGGCCCCGGGGGGAt                         <  1:32578/251‑1 (MQ=255)                                                                                                                                                                                                                             gACCATACATAAAAGAGCCGGGGATTATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGACCTGGATACTTCGGCACGTACTGCCGGGGCTCTAACCCGCCGCCGCGACATTCGTGATGCTTCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATg                        >  2:99257/1‑251 (MQ=255)                                                                                                                                                                                                                                   acataAAAAAGCCGGGGATAATTCCCATAATCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCATTTCTGGCCCAGATTGGTAAGCCGGAAGAACTGGATGCTTCGGCACGTAATGCCGGGGCTTTAACCCGCCGCCGCTAAATTCTGGATGCTGCAATTCTTCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTa                  <  1:116439/251‑1 (MQ=14)                                                                                                                                                                                                                                       aaaaaaGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGTGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTg              >  1:79444/1‑251 (MQ=37)                                                                                                                                                                                                                                          aaaGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGa             >  2:5482/1‑249 (MQ=35)                                                                                                                                                                                                                                                   gATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCAt  >  1:235228/1‑251 (MQ=32)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     GCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCAT  >  NC_000913/2514025‑2514517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.