Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,631,612	IS5 (+) +4 bp	coding (976‑979/1623 nt)	yhiJ ←	DUF4049 domain‑containing protein YhiJ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	23 (0.920)	23/492	0.2	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3631612 =	0 (0.000)					coding (979/1623 nt)	yhiJ	DUF4049 domain‑containing protein YhiJ
*	?	NC_000913	= 2290113	NA (NA)	25 (1.000)	24/492	0.2	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3631615	0 (0.000)					coding (976/1623 nt)	yhiJ	DUF4049 domain‑containing protein YhiJ

ATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/2288682‑2289162                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     aTAAACGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGTTTTTCGTATTATTGGCGGTCACATAAACCAGCTGGAGCTCTTTTT‑CCAGGACAACACCAACAACTGTCTCACCATAACCAAAAGTTGGTTGCAATGC‑ATTGGTGGTATATAAGGAAGGTGCGAAt                                                                                                                                                                                                                                          >  2:176256/1‑250 (MQ=255)              aaTACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAAttc                                                                                                                                                                                                                            <  1:71294/251‑1 (MQ=255)                   tGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCCACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGGG‑AATATAAGGAAAGTGCGAATAAGCGGGGAAATTCTtctc                                                                                                                                                                                                                       >  1:13368/1‑251 (MQ=255)                    gAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTCTTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAAACAGCTGGCTATCTTTTT‑CCCGGAAAACACCATCAACTGTCTCACCATACTCCACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCg                                                                                                                                                                                                                      >  1:247437/1‑251 (MQ=255)                          tCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTCTGGGGG‑AATATAAGGAAGGGGCGAATAAGCGGGGAAAATCTTCTCGGCTGAc                                                                                                                                                                                                                >  1:107888/1‑251 (MQ=255)                               ggcgCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAAACAGCTGGATATCTTTTT‑CCAGGACAACACCCTCAACTTTCTCACCCTACTCCACAGTTGATGCCAATGCTATGGGGG‑GATATAAGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTccgt                                                                                                                                                                                                           >  2:102633/1‑248 (MQ=255)                                           ttCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAACTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACCCCATCAACTGTCTCCCCATACTCAACAGTTGATGCCAATTCTTTGGGCG‑AATATAAGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTACGTcatttcattttt                                                                                                                                                                                               >  1:28429/1‑249 (MQ=255)                                                               cGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                                                                                                                                           <  2:87763/251‑1 (MQ=255)                                                                                                    tataTGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCCACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTTTCCCGTAAATGCCTTTAATCAGCCTTTTTAGACCg                                                                                                                                      >  2:182049/1‑251 (MQ=255)                                                                                                       atGCTGGTCGCCGCCAGGAGTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACGCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCTGCTTACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCGTGAATCAGCCCATTTAGACCGttt                                                                                                                                   <  2:214630/251‑1 (MQ=25)                                                                                                                          ttgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGGG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTTTTCATGTTTGAGCCGGTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGtt                                                                                                               >  1:193683/1‑251 (MQ=255)                                                                                                                           tgattTTCGTATTATTGGCGGTCCCATAAACCCGCTGGATATCTTTTT‑CCAGGACAAAACCATCAACTGTCTCACCATACTCAACCGTTGAAGCCAATGCTATGGGTG‑AATATAAGGAAGGGGCGAATACGGGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTTATGTTTGAGCCGCTTTTTTTTCCCGTAAATGCCTTTAATCATCCCATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGtt                                                                                                               >  2:250226/1‑250 (MQ=21)                                                                                                                           tgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGtt                                                                                                               <  1:70727/250‑1 (MQ=255)                                                                                                                                    tattatTGGCGGTCACTTACACCCGCTTGGTATCTTTTTTCCTGAACA‑CAACATCCACTGTCTCACCATACCCCACAATTTATGCCAATGCTATGGGTG‑AATATAAGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTACGACATTTCATTTCTTCATGTTTTGGCCGCGTTTTTCTTCCGTAAATTCCTTGAATCAGACTATTTAGACCTGTTCTTCTCCAATTTAAG‑TGTTATCCCCCGtt                                                                                                     >  2:241802/1‑251 (MQ=9)                                                                                                                                                          cAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTTAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTg                                                                               >  2:169687/1‑251 (MQ=255)                                                                                                                                                                                  caacaCCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGaa                                                        <  1:87473/251‑1 (MQ=255)                                                                                                                                                                                    acacCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAAc                                                      <  2:8224/251‑1 (MQ=255)                                                                                                                                                                                      acCATCAACTGTATCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAAc                                                      >  1:49661/1‑249 (MQ=255)                                                                                                                                                                                                            tCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATc                              >  2:242697/1‑251 (MQ=255)                                                                                                                                                                                                            tCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATc                              >  1:204032/1‑251 (MQ=255)                                                                                                                                                                                                                cAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCa                          <  2:145190/251‑1 (MQ=255)                                                                                                                                                                                                                     gATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTg                      <  1:182049/250‑1 (MQ=40)                                                                                                                                                                                                                                         aaTATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTGTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGGCCGCCCGAAACAGGTTGGCCAGCGTTAATAACATCGCCAGTTGGTGATCGTTTTCCAGCAAcc  <  2:247437/251‑1 (MQ=9)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTG‑AATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA‑TTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/2288682‑2289162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.