Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,429,986	IS3 (–) +5 bp :: +T	coding (34‑38/258 nt)	yrdB ←	DUF1488 domain‑containing protein YrdB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	19 (0.760) +T	18/490	0.5	100%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3429986 =	0 (0.000)					coding (38/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB
*	?	NC_000913	1094245 =	NA (NA)	29 (1.160)	27/490	0.1	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3429990	0 (0.000)					coding (34/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB

GCGAAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTGCGCTCATAC  >  NC_000913/1093998‑1094492                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        gCGAAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTGAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGa                                                                                                                                                                                                                                                       <  2:169186/250‑1 (MQ=255)   gAAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTGAACTGGCGATAATCCTGAATACGATATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACGGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATcc                                                                                                                                                                                                                                                    <  1:74452/251‑1 (MQ=255)   gAAAGCGTTAGTGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGTGCGTAATAAGGTGGT‑TGTTAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATcc                                                                                                                                                                                                                                                    <  1:147674/251‑1 (MQ=255)          ttAGCGGAAAGAATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAATGTGACGATTATTATTGGCGCGTTTGTGACTCAGGAAAATGCGTTAAAACCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACTAGGCGAAAGAGACCTGGCGTAATAAGGTGGT‑TGTGAGGTATGTTGTGAATTTTTGTGTGTAACCATAAAGCTGCATTTTGAGCCTACCCa                                                                                                                                                                                                                                              <  1:219108/250‑1 (MQ=255)           tAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCAc                                                                                                                                                                                                                                             <  1:123133/250‑1 (MQ=255)               ggAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAat                                                                                                                                                                                                                                        <  1:63530/251‑1 (MQ=255)                            gAAAATGTTGAGCGTTTAACTGGCGATCATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑AGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCCCGTAATATGGACACCGGcc                                                                                                                                                                                                                           >  2:192546/1‑251 (MQ=255)                              aaaTGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCt                                                                                                                                                                                                                          >  2:243560/1‑250 (MQ=255)                                                                                  gTGACGATTAGGATTGGCGATGTTGTGACTCAGGAAAATGCGTTAAACCAAAATGAGATCTATCGACTGGCTGATAACGCATTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGGGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTTTTCCTACCCACGTAATATGGTCACAGGCCTAAGCGAGGTTCTTGGTTGCAAAGTGTGCCGGACTGAGGCCGTCACACCAACtg                                                                                                                                                                     <  2:165398/251‑1 (MQ=255)                                                                                    gACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACtgtg                                                                                                                                                                   >  2:194087/1‑251 (MQ=255)                                                                                       gATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTgccg                                                                                                                                                                <  2:7339/251‑1 (MQ=255)                                                                                                ggtggatgttgtGACTAAGGAAAATGCGTTAACCCCAAATAAGATCGAGCGACTGGCTGATTACGCACTTTACGAGGCGAAAGAGACCGGTCGGAATAAGG‑GGTGTGTGAGGTATGGGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACTTAATATGGACACAGGCCTAAGAGAGGTTCTGGTTTTCAAATCGTTCCGTACGGAGGCCGCCATTCCAACTATACGGCCGCCACCg                                                                                                                                                       <  2:241267/245‑1 (MQ=255)                                                                                                      tgttgtGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGt                                                                                                                                                  >  1:147930/1‑250 (MQ=255)                                                                                                            gACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGAGAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTCCCCACGTAATATGGACACAGGCCAAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                                                                                                                                           <  2:214443/251‑1 (MQ=255)                                                                                                            gACTCAGGAAAAGGCGTTAAACCCAAATGAGATCTAGCGACTGGCTGATAACGCGCTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGTTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTTTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcac                                                                                                                                            <  2:94839/250‑1 (MQ=255)                                                                                                             actaaGGAAAATGCGTTAAACACAAATGATATCTATCGACTGGCTGATAACGCACTTTACGAGGGGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTACAAATTGTTCCGGACGGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACAt                                                                                                                                          <  2:215566/247‑1 (MQ=255)                                                                                                                  ggAAAATGGGTTAATCCCAAATGAAATCTATGGACTGGCTGATATCGCACTTTACGAGGCGAAATAGGCCGGGCTTAATAAGGTGTT‑TGTGAGGGATTTGGTGAATTTTTGTGAGTCACCATAAAGCGGCAGTTGAATCCTACCTACGTAACATGGGCACAGGCCTAAGTGGTGTTCTTTTGTTCAATTGGTGCCGGACGAAGGCCGCCACACGATCGGTGGCTCAGCCTCCTATTGGAATCACATTCGat                                                                                                                                     <  2:106884/251‑1 (MQ=255)                                                                                                                    aaaaTGCGTTAAACCCAACTGAGATCTATCGACTGGCTGCTAACGCACTTTACGAGGCGAGAGAGACCGGGCGTAATAAGGTGGG‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGatat                                                                                                                                   >  2:27809/1‑251 (MQ=255)                                                                                                                     aaaTGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTGCCGGACTGAGGCCGCCACTCCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGAtata                                                                                                                                  <  1:113725/251‑1 (MQ=255)                                                                                                                            ttAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAac                                                                                                                           >  2:21079/1‑251 (MQ=255)                                                                                                                               aaCCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACAcc                                                                                                                        <  1:228992/251‑1 (MQ=255)                                                                                                                                   cAAATGAGATCTATCGACTGGCTGATAACGCACCTTACGCGGCCAAAGCGACCGGCAGTACTACGGTGGT‑TGTGAGGGATGAGGTGAACTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTg                                                                                                                    >  1:12424/1‑251 (MQ=18)                                                                                                                                         agaTCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGAGTAATAAGGTGGT‑TGTGAGGGATGCGGAGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGTGGTTATTGTTTTCAAATTGTTCCGGCCTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGAAATCACATTCGATATAATTAAACACCGTTGCCCGc                                                                                                               >  1:166225/1‑250 (MQ=25)                                                                                                                                              tATCGCCTGGCTGATGACGCACTTTGCGAGCCGCCAGAGACCGGGCGTAATAAGGCGGT‑TGTGAGGGATGTGGTGACTTTTTGTGAGTCACCATAAAGCGGAATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCCCCACCGATTGTAATCACAGTCGATATAATTAAACACCGTTGCCCGCattatt                                                                                                         >  2:121311/1‑251 (MQ=18)                                                                                                                                                 cGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGGCACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACGGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTc                                                                                                       <  2:171039/250‑1 (MQ=255)                                                                                                                                                         tGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGCGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGAt                                                                                              >  2:225234/1‑251 (MQ=255)                                                                                                                                                           aTAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACTATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATa                                                                                             >  1:80525/1‑250 (MQ=255)                                                                                                                                                                           ggCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGAt                                                                            >  1:59502/1‑251 (MQ=255)                                                                                                                                                                            gCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGAt                                                                            >  1:75763/1‑250 (MQ=255)                                                                                                                                                                                            gTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCg                                                           >  1:67249/1‑251 (MQ=255)                                                                                                                                                                                                              aGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGTCTGCTAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTcc                                          >  2:136084/1‑251 (MQ=255)                                                                                                                                                                                                                 gATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACg                                       <  1:134893/251‑1 (MQ=255)                                                                                                                                                                                                                         gAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTGGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCAtt                               <  1:159711/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAACTTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTGCGCt       >  1:160203/1‑251 (MQ=17)                                                                                                                                                                                                                                                      ttttGATCCTACCCACGTAATATGGACACAGGCCTAAGCGCGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCACCCTTTTGCGCTCATAc  >  1:23995/1‑251 (MQ=11)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        GCGAAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGT‑TGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTGCGCTCATAC  >  NC_000913/1093998‑1094492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.