| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,555,047 | 2 bp→TG | coding (1963‑1964/2706 nt) | malT → | DNA‑binding transcriptional activator MalT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,555,047 | 0 | G | T | 100.0% | 35.5 / NA | 14 | V655C (GTC→TGC) | malT | DNA‑binding transcriptional activator MalT |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (6/8); total (6/8) | |||||||||||
| * | NC_000913 | 3,555,048 | 0 | T | G | 92.9% | 33.3 / NA | 15 | V655C (GTC→TGC) | malT | DNA‑binding transcriptional activator MalT |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (5/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATG > NC_000913/3554832‑3555256 || ccttccgatctgATGAAGCCGAAGCGCCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCTATGTTGATTCAATGCTCGCGGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTGGGGAAAACCTGCTGGGGAATGGCAAATATCACTGCGACTGGATCTCTAACGCCATAAAATGCCGGGTGCTTTACTGGCAAATGCCCGGCGATaa < 1:138178/239‑1 (MQ=255) ggCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGCTAAAg > 1:9974/1‑246 (MQ=255) cGAAGCGTCGGCGCGTAGCGAGATTGAAGCCTTGTCGTCTTAGCAGCCACAGCAACAGCTTCAGTGCTTGTCAAGGGTGATTCAATGCCCGCTGGCCCGTGTTGATTTAGATAACGCCCGTATCCAGCTGAACCATCTGGAAACCCGGCTGGGGAATGGCAAATATCACAGCCACTGTATCTCTATCGCCAACAAATTCCGTGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGt < 1:163456/251‑1 (MQ=255) ccTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAgc > 1:79436/1‑168 (MQ=255) ccTGGCAATGTTGANNCAATGCTCGNTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACNTGCTGGGGANNGGNAAATANCNNNGNNNCTNGATCTCTAACGCCAACAAANGCNNGGTGATNTACTGGCAAATGACCGGCGATAAAgc < 2:79436/168‑1 (MQ=255) aaTGGTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGTATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCTTCATACGGCTAAACCATAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTg < 1:226754/251‑1 (MQ=255) aTGTTGATTCAATGCTCGATGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGc < 1:25179/251‑1 (MQ=255) gATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTCACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAAc > 1:143490/1‑160 (MQ=255) aaaaCCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATNg < 1:275871/222‑1 (MQ=255) aCCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATACCGGGTGCTTTACTGGCAAATGACCGGCGATAAAGCCGCCCCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTcc > 1:145504/1‑150 (MQ=255) ctgGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATACCCGGAGTCTGCg > 1:112859/1‑251 (MQ=255) aTGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTtgatn < 1:90387/251‑2 (MQ=255) aTGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAAAAATTGTGCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTtgatn < 1:63193/251‑2 (MQ=255) gCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAANNGGTTGCNNc > 2:21617/1‑99 (MQ=255) tctAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTGTGCGAACAACCACTTCCTGCAAGGTCAAGGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTtc < 1:227401/184‑1 (MQ=255) || GGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATG > NC_000913/3554832‑3555256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |