Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,024	G→T	I162I (ATC→ATA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,024	0	G	T	100.0%	21.1 / NA	10	I162I (ATC→ATA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/5);  total (5/5)

CATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTG  >  NC_000913/2103783‑2104219                                                                                                                                                                                                                                                  |                                                                                                                                                                                                    cATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAAtttt                                                                                                                                                                                            >  1:259905/1‑251 (MQ=255)                                                                                                ttAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAgn                                                                                              <  1:89980/250‑2 (MQ=255)                                                                                                  aaaTACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCCGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCt                                                                                             >  1:190902/1‑249 (MQ=255)                                                                                                                     gggTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAAttgtt                                                                                                                                                                         >  1:196227/1‑154 (MQ=255)                                                                                                                              atTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGGTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTGGAACAAACACTGCAGTAtttttttt                                                                                                                                                <  1:354468/170‑1 (MQ=255)                                                                                                                                                        aaaTTGAGAATCATCATCAGTAAGTTGGCTTGTATCAGATAATTTAATGTCTGGCCGACTGACAATGATGGTATTTATAGAAGATTTCTTTATAAATTTGTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATTTTTATTTTATAGATCAGCCCGTATAGCATTTTAAATAATAAAAAGCTAGGTTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTa                                      <  1:133783/250‑1 (MQ=255)                                                                                                                                                                                    cTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATCGCATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCtt                                                          >  1:156579/1‑202 (MQ=255)                                                                                                                                                                                           cAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATATTTGTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTATTCTCCATAAGAATTTTACGGAATAAAATTCCTTTATAAATAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTg  <  1:360021/251‑1 (MQ=255)                                                                                                                                                                                                  tttaatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGcc                                                                                                                         <  1:299979/125‑1 (MQ=255)                                                                                                                                                                                                               ngACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAATTAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACatat                       >  1:212429/2‑210 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                    CATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTG  >  NC_000913/2103783‑2104219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑