Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,090,780	C→T	Q197* (CAA→TAA)	hisG →	ATP phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,090,780	0	C	T	100.0%	14.4 / NA	7	Q197* (CAA→TAA)	hisG	ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/5);  total (2/5)

TCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCACGCTGGAAGCTAACGGCCTGCGCGAAGTCGAAGTTATCTATCGCTCGAAAGCCTGCCTGATTCAACGCGATGGCGAAATGGAAGAATCCAAACAGCAACTGATCGACAAACTGCTGACCCGTATTCAGGGTGTGATCCAGGCGCGCGAATCAAAATACATCATGATGCACGCACCGACCGAACGTCTGGATGAAGTCATCGCCCTGCTGCCAGGTGCCGAACGCCCAACTATTCTGCCGCTGGCGGGTGACCAACAGCGCGTAGCGATG  >  NC_000913/2090651‑2090986                                                                                                                                  |                                                                                                                                                                                                               tCTGTTGAAGTCCCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTTGTTTCCACCGGTGCCACGCTGGAAGCTAACGGCCTGCGCGAAGTCGAAGTTATCTATCGCTCGAAAGCCGGCCTGATTTAACGCGATGGCGAAATGGAAGAATCCAAACAGCAACGGATCGACAAACTGGTGACCCGGATTCAGGGTGTTATCCAGGCGCGCGAATCAAAATACATCATGATGCACGCaccg                                                                                               <  1:47455/243‑1 (MQ=255)          gTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCACGCTGGAAGCTAACGGCCTGCGCGAAGTCGAAGTTATCTATCGCTCGCAAGCCTGCCTGATTTAACGCGATGGCGAAATGGAAGAATCCAAACAGCAACTGATCGACAAACTGCTGACCCg                                                                                                                                                      >  1:262088/1‑179 (MQ=255)          gNCGCCCCGCGTGCCGGACTGGCGGATGCGATTNGNGATCTGGTTTCCACCGGTGCCACGCTGGAAGCTAACGGCCTGCGCGAANTCGAAGTTATNTATNNCTCGNANGNCTGCCTGATTTAACGCGATGGCGAAATGGNAGAATCCAAACAGCAACTGATCGNNAANNNGCNNACCCg                                                                                                                                                      <  2:262088/179‑1 (MQ=255)                                                   gnNTTCCACCGGTGCCACGCTGGAAGCTAACGGCCTGCGCGAAGTCGAAGTTATCTATCGCTCGAAAGCCTGCCTGATTTAACGCGATGGc                                                                                                                                                                                                     <  2:14944/91‑1 (MQ=255)                                                    gTTTCCACCGGTGCCACGCTGGAAGCTAACGGCCTGCGCGAAGGCGAAGTTATCTATCGCTCGAAAGCCTGCCTGATTTAACGCGATGGCGAAATg                                                                                                                                                                                               <  1:165833/96‑1 (MQ=255)                                                                     gCTGGAAGCTATCGGCCTGCGCGAAGTCGAATTTATCGATCGCTCGAAAGCCTGCCGGATTTAACGCGAGGGCGAAATGGAAGAATCCAAACAGCAACTGATCGACAAACTGCTGACCCGTATTTAGTGTGTGATCCAGGCTCGCGAATCAAAATACATCATGATGCACGCTACGACCGAACGGCTGGAGGAATTCATCGCTCTGCTGCCAGGTGCCGAACGCCCACCTATTCTGCCGCTGGCGGGTGAcc                   <  1:263995/251‑1 (MQ=255)                                                                                          ngaagtcgaagtTATCTATCGCTCGAAAGCCTGCCTGATTTAACGCGATGGCGAAATGGAAGAATCCAAACAGCAACTGATCGACAAACTGCTGACCCGTATTCAGGGTGTGATCCAGGCGCGCGAATCAAAATACATCATGATGCACGCACCGACCGAACGTCTGGATGAAGTCATCGCCCTGCTGCCAGGTGCCGAACGCCCAACTATTCTGCCGCTGGCGGGTGACCAACAGCGCGTAGCGATg  >  1:85946/2‑247 (MQ=255)                                                                                                                                  |                                                                                                                                                                                                               TCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCACGCTGGAAGCTAACGGCCTGCGCGAAGTCGAAGTTATCTATCGCTCGAAAGCCTGCCTGATTCAACGCGATGGCGAAATGGAAGAATCCAAACAGCAACTGATCGACAAACTGCTGACCCGTATTCAGGGTGTGATCCAGGCGCGCGAATCAAAATACATCATGATGCACGCACCGACCGAACGTCTGGATGAAGTCATCGCCCTGCTGCCAGGTGCCGAACGCCCAACTATTCTGCCGCTGGCGGGTGACCAACAGCGCGTAGCGATG  >  NC_000913/2090651‑2090986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑