| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,083,303 | C→T | A183V (GCG→GTG) | sbcB → | exodeoxyribonuclease I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,083,303 | 0 | C | T | 94.7% | 45.6 / ‑6.3 | 19 | A183V (GCG→GTG) | sbcB | exodeoxyribonuclease I |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/9); minor base G (0/1); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
GTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGCGGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAA > NC_000913/2083086‑2083533 | gtcccacGCAACATTTTTTATCGTAATTTCTACGATCCTTCCGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTTCGCCCGGAAGGAATAAACTGGCCTGAAAATGATTACCTTCTACCGAGCTTTCGCCTTTATCATTTAACCAAATCGAATGGTATTGAACATAGCAACGCCCAcgatgtgat < 1:164062/217‑1 (MQ=255) gTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTTAACATAGCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAATCGCGTCan < 1:71040/251‑2 (MQ=255) gTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGGTATGCACTGCGCCCGGACGGCATAAACTGGCATGAGAATGCTGAGGGTATACCGAGCTTTCGCCTTGAGCATTTCACCAACGCGAATGGTATTGAACATAGCAACGCCCCCGATGTGATGGCTGATGTGTACGCCACTATTGCGCTGGCACAGCTGGTAAAAACGCGTc > 1:343800/1‑249 (MQ=255) aCTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGCCGGTCTCCCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGAATTGAACATAGCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTCCCCATCGTAATAAac > 1:226947/1‑249 (MQ=255) cNTGCCTGTTATGCCCTGCGCCCGGAAGGAATCAACTGGACTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGACCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGc > 1:278466/1‑250 (MQ=255) ccTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGtaataa > 1:45782/1‑204 (MQ=255) cccGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGACTGGTATTGAACATAGCAACGCTCACGATGTGCTGGCTGATGTGTACGCCACTATTGCGAt > 1:326504/1‑135 (MQ=255) ccGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGTGATGGc < 1:129036/110‑1 (MQ=255) aaTAAACTGGCCTGAAAATGATGACGNTCTACCNNGCNTTCGCCTTGAGCATTTAACNAANNNGNNNGNTATTGNACNNAGCAACGCCNACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAANCGCGTAAGCNNCGCCTGTTTGATTATCTCTTTNCCCNNCNNANTAAnc > 2:81286/1‑191 (MQ=255) aaTAAACTGGCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGGGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTcc < 1:144364/219‑1 (MQ=255) ggCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTgg < 1:282235/250‑1 (MQ=255) aaaaTGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATgg < 1:81286/251‑1 (MQ=255) gatgaCGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGcgcg < 1:162027/250‑1 (MQ=255) ttCGCCTTGATCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGTGATGGCTGATTTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTTATTATCTCTTTTCCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCATATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGCGGCAATACCAGCTGGGTGGc < 1:225640/250‑1 (MQ=255) ncGAATGGTATTGAACATAGCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGaa > 1:91607/2‑167 (MQ=255) aTTGAACATAGCAACACCCACGATGTGATGGCTGATGTTGACGCCTCTATTGCGATGGCAAAGCTTGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCGCTTTACCCAGCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGAGGAAACCCCTGGTGCACGTTTCCGGAATGGTTGGAGCATGGCTCGGCAATACCAGCTTGGTGGCACCGCTTGCGTGGCATCCTGAAAATCGCAATGc < 1:249150/251‑1 (MQ=255) gCAACGCCCACGATGTGATGGCTGATGTGTATGCCACTATTGCGATGGCGAGGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAGACTGATGGCGTTGAGTGATGTTCCGCAGATTAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGCGGCAATAc < 1:167094/196‑1 (MQ=255) gCAACGCCCACGATGTGATGGCTGATGTGTACGCNACTNTTGCNATNGCAAAGCTGGTAAAANCGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATNNANNNANACTGATgg < 2:244576/123‑1 (MQ=255) gCAACGCCCACGATGTGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGACACGCCTGTTTGCTTATCTCTTTACCCATCGTAATAAACACAAACTGATgg > 1:244576/1‑123 (MQ=255) nccACGATGTGATGGCTGATGTGTACGCCCCTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGCGGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTaa > 1:102482/2‑240 (MQ=255) | GTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGCGGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAA > NC_000913/2083086‑2083533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |