Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,076,331	C→T	L8L (CTC→CTT)	yeeS →	RadC‑like JAB domain‑containing protein YeeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,076,331	0	C	T	100.0%	30.1 / NA	12	L8L (CTC→CTT)	yeeS	RadC‑like JAB domain‑containing protein YeeS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/9);  total (3/9)

GAACAGAAATCCAGACGAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACC  >  NC_000913/2076118‑2076569                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               gAACAGAAATCCAGACTAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAACCTTATTCAACGGGCCCTGAAACCCCTGGACCGCCATCTTc                                                                                                                                                                                                           <  1:183422/251‑1 (MQ=255)                              aCTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTTATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCAcc                                                                                                                                                                              <  1:345472/251‑1 (MQ=255)                                                                          aCAGTATCGTTACATGGGAAATAATGGCCCCATGCAAATGGCAGTCCCCCGAGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTTAtt                                                                                                                                                                                                                                             >  1:198964/1‑144 (MQ=255)                                                                                           gAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTGGAAACCATGATGAATAAGATGCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTTATTCAACGGGCCCTGAAAACCCTg                                                                                                                                                                                                                        <  1:186601/148‑1 (MQ=255)                                                                                                             aaCTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTTTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTATAGGAGAGATGACGCCCGGCGAGCGCAGCCTTATTCAACGGTCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTc                                                                                                                                                                                  <  1:334628/168‑1 (MQ=255)                                                                                                                       nncccNTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTTATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAAc                                                                                                                                                                                                     <  2:211881/137‑1 (MQ=255)                                                                                                                         cccGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTTATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGCCTGGAGCGTGAAGAATTCCGGGGGCTGGATCTGAACAACCAGAATCAGCTg                                                                                    >  1:281929/1‑250 (MQ=255)                                                                                                                            gTGATCAGTAGGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGAAGCCCGGCGAGCGCAGCCTTATTCAACGGGCCCTGAAAAACCTGTACAGCCATCTGCATTAAGCCGGCGTTGCCGTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGAGGGACGGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTg                                                                                <  1:193648/251‑1 (MQ=255)                                                                                                                                                gTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTTATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCan                                                             <  1:39320/250‑2 (MQ=255)                                                                                                                                                                   aNATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCNGCGNNCGNNGCNTTAtt                                                                                                                                                                                                                                             <  2:198964/55‑1 (MQ=255)                                                                                                                                                                                        aggagAGATGACGCCCGGCGAGCGCAGCCTTATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTTAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAATCGCACGGAAGGCCAGCCCCGGGAAGTGa                    <  1:111990/251‑1 (MQ=255)                                                                                                                                                                                                          cGAGCGCAGCCTTATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTAcc  >  1:99684/1‑251 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               GAACAGAAATCCAGACGAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACC  >  NC_000913/2076118‑2076569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑