Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,052,885	G→A	intergenic (+871/‑758)	yeeJ → / → shiA	inverse autotransporter adhesin/shikimate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,052,885	0	G	A	100.0%	46.8 / NA	19	intergenic (+871/‑758)	yeeJ/shiA	inverse autotransporter adhesin/shikimate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/8);  total (11/8)

GTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATTAAAGACTTCT  >  NC_000913/2052671‑2053125                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 gTTCCTGCGAGCTACCTTCTCAATTCAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACcaa                                                                                                                                                                                                                            >  1:115506/1‑237 (MQ=255)                    aaaaTGGAACTCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGCGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACTCAGCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAGCCTGAAAATTCGTTTTTGCTTTCAactgac                                                                                                                                                                                           <  1:295672/250‑1 (MQ=255)                                                                                             ggCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATACATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGCTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTc                                                                                                                                                                                                              >  1:195440/1‑159 (MQ=255)                                                                                                    cATATTTTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTCAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTGTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATg                                                                                                           <  1:325915/251‑1 (MQ=255)                                                                                                    cATATATTGGCCTGACTGCTGGAAAACCCGCCTCACTCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGATTTTCTGATCCATACTGGACGCGTTCTTACACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAAtt                                                                                                                                                                                                               <  1:11236/151‑1 (MQ=255)                                                                                                             gCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGc                                                                                                                    <  1:343586/233‑1 (MQ=255)                                                                                                                     nctgGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCttt                                                                                          >  1:286212/2‑251 (MQ=255)                                                                                                                     gctgGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAg                                                                                                                                                                                                                                               >  1:118810/1‑102 (MQ=255)                                                                                                                      ctgGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCa                                                                                                                                                                      <  1:136883/174‑1 (MQ=255)                                                                                                                      ctgGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCAGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactg                                                                                                                                                                                             <  1:192181/151‑1 (MQ=255)                                                                                                                                              tAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACACGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGCCTGATAACTAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGCTCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATcc                                                                 >  1:201856/1‑251 (MQ=255)                                                                                                                                                    cccATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACTAACACCCTGAACATTCGTTTTTGCTTTCAACTGACTGATACATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGATGCGTGATCCTGCAt                                                            >  1:247563/1‑250 (MQ=255)                                                                                                                                                     ccATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACACAGGGGAAACGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCAt                                                            >  1:195865/1‑249 (MQ=255)                                                                                                                                                                           cGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCCATTAATGGTTATACCAACAACCTGAAAATTCg                                                                                                                                                                                                             >  1:170254/1‑82 (MQ=255)                                                                                                                                                                                                     gTTCTTGCACAAAGGGGAAAAGCAATTCATTGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACGGACTGATAAATAACATCGTTTTCAGGTTCTTATTACGCATCCCCTCAATGCAAAGATCCGGCGTACCTTATTTCTGTGTTATTGTCTTTGCTAAATCTTGTATTTCTTTTAATGTTGCTTGATCCTTCATTGTCATTGTTCCTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATTaa          <  1:359307/251‑1 (MQ=255)                                                                                                                                                                                                     gNTCTTGCACAAAGGGGAAAAGCAATTCATGGTTCTANCAANNACNNTANAATTc                                                                                                                                                                                                              <  2:195440/55‑1 (MQ=255)                                                                                                                                                                                                           gCACAAATGGGAACAGCAATTCATGGTTATCCCAACAACCTGCAAATTCGTTTTTGCTTTCAAATGCCTGCTAAATAACATCGATTTACGGTTCCTTTTACGCCTCCCCTCAATGCAACGATCACGCGTAACGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAAAGTTAATTTAGTCTGTTCAAGTTTAAGCACATTACAGa       >  1:45347/1‑248 (MQ=255)                                                                                                                                                                                                            cacaAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGCCTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCACAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTACTTTAGTCTGTTCAAGTTTAAGCGCATTAAAg        >  1:159070/1‑246 (MQ=255)                                                                                                                                                                                                               naaGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATTAAAGAcagat  >  1:89537/2‑245 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 GTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATTAAAGACTTCT  >  NC_000913/2052671‑2053125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑