Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,040,4330CA94.4% 40.9 / ‑6.9 18A319D (GCC→GAC) msrPprotein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (7/10);  minor base T (0/1);  total (7/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

ATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTT  >  NC_000913/2040209‑2040661
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    
atatGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGTCATCGCTGTATCGTGGcc                                                                                                                                                                                                            <  1:98561/251‑1 (MQ=255)
  atGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCTAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTg                                                                                                                                                                                                          <  1:244719/251‑1 (MQ=255)
                                            cccGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTc                                                                                                                                                                              >  1:247287/1‑237 (MQ=255)
                                                       ccGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATgga                                                                                                                                                      <  1:18511/250‑2 (MQ=255)
                                                         gCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTg                                                                                                                                                    >  1:262150/1‑250 (MQ=255)
                                                                    nnnnnntNGCNNCGCCNGACGAATACGGTTTTTACGCCAACNTTAATCCGTATGTTGATCACCCGCGCTGGTCNCNGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGNCAGCCAACGCNACTNNTTAANGNTNACGCCGACCAGGTGGCATCGCTGTATCGTNGCCTGGATTTGCGGGAGAATTTCNNAANNNGTNNGACAg                                                                                                                                                                        <  2:262891/213‑1 (MQ=255)
                                                                             naGCGCCTGACGAATACGGGTTTTACGCGAACGTTAACCAGTATGTTGAACACACGCGCTGGTCACAGGCTACAGAACGATTTCTAGGTACAGGCGACATACTAGATGCACAGAGCAAGCCAACGCGACAGTTTACTGGTTACGCCGACCAGGTGGAATCGCTGTATCGTGGACTGGCTTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGGGACATGGCTGAAAGTTTGCCTGCATCTTGcc                                                                                                                               >  1:42555/2‑251 (MQ=255)
                                                                                                                                         ggTCACAGGCTACCGAACGATTTATTGGTGCAGGCGGCATCCTCGATGTACAGCGCCAGCGAACGCTACTGTGTAATGGTTACGCCGTCCAAGTGGCATCGCTGTATCGTGGCCTGGATTGGCGGTAGAATTTCTAAATGCGTGTGACAGATAAACAGGTGTCATGGCTTAATGTCGGCGTTCATCTTGCCGGGGTGTTGCGGTTTCTCCGTGTGGTCTGGGCGATCAATCGCGGTGGACTGGGTGCCGa                                                                    <  1:329555/250‑1 (MQ=255)
                                                                                                                                                ggCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCAACGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGGTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGc                                                                       <  1:349514/240‑1 (MQ=255)
                                                                                                                                                      cGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCTGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTg                                                                        <  1:246708/233‑1 (MQ=255)
                                                                                                                                                              ntntgnnttCANGCGGCATCCTCGATGTACAGCGCCNGCCAACGCTACTGTTTAATGGTTACGCCGACNANGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGNGTCTGACAGCNAAANNGGTGNCNTNGCTGAAAGTTTGCCTGCATCTTGCCGGATNGTTGCCGTTTCTCTGGCTGGTCTNNGCNNNCANNCACgg                                                                                   <  2:262150/207‑1 (MQ=255)
                                                                                                                                                                        aGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGACGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGAGCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTc                                    <  1:110245/251‑1 (MQ=255)
                                                                                                                                                                            ggcATCCTCGCTGTACAGCGCCAGCCACCGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCgcac                                >  1:94335/1‑251 (MQ=255)
                                                                                                                                                                                        ntACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTg                                                                                                                                        >  1:214917/2‑135 (MQ=255)
                                                                                                                                                                                        gTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGACGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAAtt                                                                                                                                                                                          >  1:237425/1‑85 (MQ=255)
                                                                                                                                                                                        gNACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTg                                                                                                                                        <  2:214917/135‑1 (MQ=255)
                                                                                                                                                                                                            aCTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTTGCGACCTTGtt  <  1:12558/249‑1 (MQ=255)
                                                                                                                                                                                                                  tAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGNNATGGCTNna                                                                                                                                                  >  2:10300/1‑99 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    
ATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTT  >  NC_000913/2040209‑2040661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑