Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,023,559	A→G	T223A (ACT→GCT)	fliR →	flagellar biosynthesis protein FliR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,023,559	0	A	G	100.0%	12.6 / NA	7	T223A (ACT→GCT)	fliR	flagellar biosynthesis protein FliR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/4);  total (3/4)

GATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAG  >  NC_000913/2023366‑2023775                                                                                                                                                                                                  |                                                                                                                                                                                                                         gATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGCATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCCTTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGAGGCATTAATGCCGTTAATTGCACCttttttc                                                                                                                                                                     >  1:29956/1‑245 (MQ=255)                        gCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCAtctn                                                                                                                                                                                                             <  1:212142/184‑2 (MQ=255)                                       cACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGTTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGGCACTTAATCGGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATGTTTGTTATTGTATTTCCATTTACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAAtn                                                                                                                                                                                          <  1:40985/188‑2 (MQ=255)                                                                                                        ctgctgctgACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCAATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTCATGACGTTAATTTCACCTTTTTGCGAAAATTTATTCAGTGACATTTTTAATTTGCTCGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCCTGTTATCCTAAGGATTATACGaa                                                           >  1:237424/1‑250 (MQ=255)                                                                                                                nacacTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAATATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCAGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATAc                                                  >  1:62960/2‑251 (MQ=255)                                                                                                                                    gTTTACTTAATCGTATTGTCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCAGTAATGCCGTTAATTGCACCTTTGTGCGAACATTTATTCAGTGAAAGTTTTAATGTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTTACGTTTATCATGGTATCCTAAGGATTATCCGAAAAATAATACCTACGAAc                                          <  1:321002/239‑1 (MQ=255)                                                                                                                                                                                    ttCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACGCCTGCAGCGAAATATTTGTTTTAAg  <  1:272863/231‑1 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                         GATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAG  >  NC_000913/2023366‑2023775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑