Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,002,825	C→T	G261E (GGA→GAA)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,002,825	0	C	T	100.0%	43.4 / NA	16	G261E (GGA→GAA)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

ACAGCACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTCCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAG  >  NC_000913/2002591‑2003005                                                                                                                                                                                                                                           |                                                                                                                                                                                     aCAGCACCAGTAGTTTCATTCACATCCGCAGCGTCAACATTGCCATTTGTATCTTTAAGCGCATATCTATCGGAATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGCAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGcc                                                                                                                                                                              >  1:140226/1‑243 (MQ=255)          gtagtTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTc                                                                                                                                                                        >  1:302157/1‑240 (MQ=255)                 cattcaCATCCGCAGCGTAAAGATTGCAATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGc                                                                                                                                                       >  1:26165/1‑250 (MQ=255)                                                          aGCGCATATGTATCGGTATCCTTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCACCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACcatca                                                                                                                                                            >  1:118894/1‑204 (MQ=255)                                                                                 aCCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTg                                                                                                            >  1:308329/1‑229 (MQ=255)                                                                                      tGGAATCCTTCAGTTTTCCTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTTAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGGAGTATTTGCATCAGTTGCAGGGGCATGTGCCGTTGCTTCAGGCGCCATTGTCACCGGTCCATCATTAGCAACTGTTACTGCGTAATCCTTCCCATCGTTTTCACCACCGGTGATTTTCGCATAGTAATCATTACCAtt                                                                                  <  1:157327/250‑1 (MQ=255)                                                                                          aTCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGAGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATc                                                                               >  1:21396/1‑249 (MQ=255)                                                                                                                aCAGCACCAAGGTTGGCAGTTGCGGATCCTGCAGTATTATCAATCTGAACAGGTGTGCCGCCTGAAGTGATATTTGTAGCTTTAGTAGTATTTGTATCGGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCAttn                                                                                                                                                                          <  1:43822/136‑2 (MQ=255)                                                                                                                      ccAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTCGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGt                                                                                                                                         >  1:327329/1‑163 (MQ=255)                                                                                                                                           ccTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATGGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGAGTTTCTCATAGTTATCATTGCCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTggn                            <  1:66085/251‑2 (MQ=255)                                                                                                                                                      atCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTgggg                                                  <  1:95640/218‑2 (MQ=255)                                                                                                                                                         aaTCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAacacn                                                                   <  1:67177/198‑2 (MQ=255)                                                                                                                                                                 cAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCg                                                                                                                                                                                <  1:261569/81‑1 (MQ=255)                                                                                                                                                                      gtACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCAGAGGAATCATTACCATTAGCAGTAGAAGCACCGTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAg  <  1:174223/250‑1 (MQ=255)                                                                                                                                                                             cTGAAGTGATCGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGAATTTGCCGTTGCTTCAGTAGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGt                                                                            >  1:244643/1‑169 (MQ=255)                                                                                                                                                                             cTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTNTACNATCANTANCAACTGTTACTGCGTNATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATNNTNNNCNTTATCAGt                                                                            <  2:244643/169‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                     ACAGCACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTCCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAG  >  NC_000913/2002591‑2003005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑