Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	47.2 / NA	19	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/8);  total (11/8)

GCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCT  >  NC_000913/1894628‑1895064                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  gcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCtt                                                                                                                                                                                            >  1:119565/1‑251 (MQ=255)      tGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGATAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCGGTCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGc                                                                                                                                                                                        <  1:353992/250‑1 (MQ=255)                                                      naCAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACCTTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTccgg                                                                                                                                                                                                                        >  1:73310/2‑167 (MQ=255)                                                          gNTTTTTGCTGATGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGtat                                                                                                                                     >  1:277960/1‑249 (MQ=255)                                                           cTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCACACATTGTTAACTCCTGCTAAATTGGTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCCCCTGCCGTGGGCGCTGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGc                                                                                                                                  >  1:307923/1‑251 (MQ=255)                                                                             gggTGAGTGAAGGGAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGc                                                                                                                                                                                        >  1:2198/1‑179 (MQ=255)                                                                                        ngTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGATACCCGGCACATAGCAAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAg                                                                                                                                                                                         >  1:73044/2‑167 (MQ=255)                                                                                           aaaCCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTg                                                                                                  >  1:10528/1‑251 (MQ=255)                                                                                                        ttGTTACCTCCTGCTAAATTGTTGGTNCTGCTTNNTTNCTGCTACCTGGCACATAGCNAGNNNANNNANGACTGNTGNNGACGTTATCNCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCNCGCTTAAGCNNCCTGCCGTGGGCGATGCTTTTNCACNNCNNCNATGNCgn                                                                                                                                               >  2:75920/1‑192 (MQ=255)                                                                                                            tAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCATTTTc                                                                                  <  1:75920/250‑1 (MQ=255)                                                                                                                                                         cacaTAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGACGCTGACTTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGGTAATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGAcc                                     >  1:255760/1‑250 (MQ=255)                                                                                                                                                            aTAGCNNGTNNNGCNNGGACTGATGAAGACGTTATCTCCNGCTGTGATTACTTTCCCATGGCGTCAGGan                                                                                                                                                                                                                      >  2:259778/1‑69 (MQ=255)                                                                                                                                                              agccagtagatatatgACTTATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTAGCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACACCGGCTATGCCGATCATCCGTATAGCCGCTGTGATATTGTGGTCGCCGAGCCGATTTGCACTGTAACCTCGTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACtga                                <  1:305720/235‑1 (MQ=255)                                                                                                                                                                ccAGTANNGTCAGGACTGATGAAGACGTTATCTCCCTCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGc                                                                                                                                                                                        <  2:2198/96‑1 (MQ=255)                                                                                                                                                                        gTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGACTATGCCGATCATNCGtn                                                                                                                                      >  2:221099/1‑137 (MQ=255)                                                                                                                                                                           aGGACTGATGAAGACGTTATCCCCCGCTGTGATGACGTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTAAAGCAGCTTTGATATTGTGGGCTCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGGGAAAAACGCACAACGACCACTGATGACCCGCTACAgg                  <  1:303227/251‑1 (MQ=255)                                                                                                                                                                            ggACTGATGAAGACGTTATCTCCCGCTGTTATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTTCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAggn                 <  1:38927/251‑2 (MQ=255)                                                                                                                                                                            ggACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTGCCCTGGCGTCAGGACGCCGCTGAATTTTATGTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTTCACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACGTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGc                                                       <  1:147018/213‑1 (MQ=255)                                                                                                                                                                                            tagatcCCGCTGTGATTACTTTCCCCCGGCGTCAGGACGCCGCTGAATTTTATTCCTCCCGTTTAAGCCACCTGCCGTGGGCGATGCTGTTACTCTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGGCGCCGAGCCGATTTGCACTCTAACCTCTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGCCCCGCTACAGGTGCTCTAGCAGGTGcn  <  1:221099/246‑2 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  GCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCT  >  NC_000913/1894628‑1895064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑