Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,836,397	G→A	E109K (GAG→AAG)	ynjB →	putative ABC transporter periplasmic binding protein YnjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,836,397	0	G	A	100.0%	35.3 / NA	14	E109K (GAG→AAG)	ynjB	putative ABC transporter periplasmic binding protein YnjB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/5);  total (9/5)

AAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAGAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTG  >  NC_000913/1836152‑1836598                                                                                                                                                                                                                                                      |                                                                                                                                                                                                          aaaaTGAGGCCAAAGGGCAAACCGGCTGGTTTAACGCCTGGGGCGGCGAGACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGATGTAAAACGGGCGGCGCGGTGGATCTGCGCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGcc                                                                                                                                                                                                      <  1:352391/251‑1 (MQ=255)           cAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACCGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTAc                                                                                                                                                                                            >  1:27200/1‑251 (MQ=255)                                           gcggcgATACCGCAATTAACCGCTATCTCGACTGGGCGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTgg                                                                                                                                                            >  1:290676/1‑251 (MQ=255)                                                               ngCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCt                                                                                                                                        >  1:63976/2‑251 (MQ=255)                                                                         aCTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCgg                                                                                                                              <  1:279323/251‑1 (MQ=255)                                                                                       nagaTGAAAACCCATTACGCTATAAACCTGAAGCTTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGCCGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCtgt                                                                                                                >  1:35272/2‑251 (MQ=255)                                                                                                                     aaGATTGTCCGGCGGGCGGAGGCCGCAGACGCGGGGAAGCGCATGAAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGTGCGGGGGATCGGGTCTGGGTGAACGGCGAAAACTCCCGCACCGTAAAAAAGGCCAATTTAGTACAAACGGGCTGGGCGTATACTCGGCCCAATTGGCGTTATGTCGACACACAGGTTCCGGTGCGGGAAGATTTTTCTGGGCCGACACAAGGTGCGGACTCGCCCTgggg                                                                                  <  1:138883/251‑1 (MQ=255)                                                                                                                                                                    gACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCNATGNNNnnacnnnnnn                                                                                                                                        >  2:218906/1‑144 (MQ=255)                                                                                                                                                                                                    ncTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCCATTTATTACAAACGGGCTGGGCGGAGAc                                                                                                                                                                         >  1:37288/2‑85 (MQ=255)                                                                                                                                                                                                    gnTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGG‑CGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGtatg  >  1:277766/1‑248 (MQ=255)                                                                                                                                                                                                        nntGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAANGCCAANTNANNACNNACGTGCTg                                                                                                                                                                                  <  2:224844/70‑1 (MQ=255)                                                                                                                                                                                                               cTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCNNTATGTCNnc                                                                                                                                                >  2:15797/1‑99 (MQ=255)                                                                                                                                                                                                               cTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGacacac                                                                                                                                            <  1:15797/103‑1 (MQ=255)                                                                                                                                                                                                                               gCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATATCGACACACAGCTGCCGGTGCGGGAAGATTTTTc                                                                                                                   >  1:111013/1‑112 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                          AAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAGAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTG  >  NC_000913/1836152‑1836598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑