Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,799,792	C→T	intergenic (‑43/+10)	rplT ← / ← rpmI	50S ribosomal subunit protein L20/50S ribosomal subunit protein L35

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,799,792	0	C	T	100.0%	87.2 / NA	31	intergenic (‑43/+10)	rplT/rpmI	50S ribosomal subunit protein L20/50S ribosomal subunit protein L35
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/12);  total (19/12)

GATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAA  >  NC_000913/1799555‑1800004                                                                                                                                                                                                                                              |                                                                                                                                                                                                                     gATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTAt                                                                                                                                                                                                         >  1:298913/1‑251 (MQ=255)       cgctaTCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGGGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTGACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTAc                                                                                                                                                                                                   <  1:356211/247‑1 (MQ=255)        ncAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACg                                                                                                                                                                                                  >  1:214305/2‑251 (MQ=255)         cAATCCACAGTTGACGGAACTGACACTTACGTTGCCGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACgg                                                                                                                                                                                                 >  1:131016/1‑251 (MQ=255)              cacaGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTAGACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACggcaggn                                                                                                                                                                                            <  1:73358/251‑2 (MQ=255)                     gACGGAACTGACGATTACGTTGATGACGGTCCCGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACACGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGAt                                                                                                                                                                                     >  1:271311/1‑251 (MQ=255)                                                   naCGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTCTCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGCTTACCAGGCCCAGATCGCCTTTGGAAACCAt                                                                                                                                                       >  1:210049/2‑251 (MQ=255)                                                     cGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTAGGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATgg                                                                                                                                                     <  1:203737/251‑1 (MQ=255)                                                           gCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACg                                                                                                                                                                                                  <  1:28060/200‑1 (MQ=255)                                                           gCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCTATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCg                                                                                                                                               <  1:186629/251‑1 (MQ=255)                                                                                  aGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGAAGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTCATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTgg                                                                                                                                                              >  1:205308/1‑213 (MQ=255)                                                                                       ggAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCATGTTTCACAATTTTCTTGTGACGTGCACGTGCAATAACACAACGTTTTACGCGAGCCATCTGTGCTCTCCTGTATCTATATTATAATTAAAAAGTTAAAAATGTTAACGGCTTATg                                                                                                                                                                                                        >  1:338851/1‑166 (MQ=255)                                                                                             cAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTCAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCtttttt                                                                                                             >  1:345491/1‑251 (MQ=255)                                                                                                              gaACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAACTTTTCTTGTGACGCGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGc                                                                                                                                                    >  1:127477/1‑195 (MQ=255)                                                                                                                   cgcACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTATAACGTGCACGTGCAATCACACAACGTTTTACGAGAGCCATATGTGCTCTCCTGTATCTATATTCTACTTAAAAAGTTAAAAATGTTAACGACTTATGCGTACGGCACGCACGCGATTACCAGGCCCAGATCGCCt                                                                                                                                                                  >  1:138513/1‑176 (MQ=255)                                                                                                                     cACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTGTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATTCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGc                                                                                                                                                    <  1:291972/188‑1 (MQ=255)                                                                                                                                ncTTTAGCTTGTTTCAAAATTTTCTTGTGAAGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTCATGCGTACGGCAGGCACGCGATTACCAGGc                                                                                                                                                                             >  1:105094/2‑152 (MQ=255)                                                                                                                                                                 gcacgtgcaATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAACATGTTCAAGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGCCGCAGGTGACGTTTACGTTTGGTCGCTTTTTTg                                                                                                            >  1:320767/1‑184 (MQ=255)                                                                                                                                                                     ntgcaATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAg                                                                                      >  1:220378/2‑202 (MQ=255)                                                                                                                                                                     gtgcaATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTagc                                     >  1:146311/1‑251 (MQ=255)                                                                                                                                                                      tgcaATAACACCACGTTTTACGCGAGCCATATGAGCGCTCCTGTATCTATATTCTAAGTAAAAAGTTAAAAATGGTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTGTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTc                                                                                                         <  1:356431/182‑1 (MQ=255)                                                                                                                                                                                ccacGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGt                           >  1:274971/1‑250 (MQ=255)                                                                                                                                                                                    gTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTAGGACNCAGNNNnngtnnnnnn                                                                                                                           >  2:216821/1‑144 (MQ=255)                                                                                                                                                                                               gCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCgg                                                        <  1:113983/206‑1 (MQ=255)                                                                                                                                                                                                 cATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGACCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCCGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGc         >  1:307821/1‑251 (MQ=255)                                                                                                                                                                                                         ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACgcgcaaacgcgtcgcgcaatggc  >  1:95487/1‑228 (MQ=255)                                                                                                                                                                                                                    cTNTATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTc                                                                                                         <  2:210049/136‑1 (MQ=255)                                                                                                                                                                                                                         nnnnnnttNNNNNGTTNAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAg                                                                                      <  2:220378/144‑1 (MQ=255)                                                                                                                                                                                                                              aTTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACATTTGGTCGCTTTTTTGGTCAGAATGTNACgnnnnn                                                                                         >  2:223321/1‑137 (MQ=255)                                                                                                                                                                                                                              aTTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACATTTGGTCGCTTTTTTGGTCAGAATGTGACGCAggn                                                                                         <  1:223321/142‑2 (MQ=255)                                                                                                                                                                                                                                nanaanGTTAANAATGNTAACGGCTTATGCGTNCGGGAGGCGCGGGATTTCTNNGNCCAGANCGCCt                                                                                                                                                                  <  2:138513/66‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                     GATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTGTGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAA  >  NC_000913/1799555‑1800004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑