Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,748,841	C→T	R240K (AGA→AAA)	ydhT ←	uncharacterized protein YdhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,748,841	0	C	T	100.0%	51.8 / NA	19	R240K (AGA→AAA)	ydhT	uncharacterized protein YdhT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/8);  total (11/8)

CGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCA  >  NC_000913/1748596‑1749080                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                cGTTCGGCGCGTTGCCCTGGTTGATACACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCg                                                                                                                                                                                                                                            <  1:149673/251‑1 (MQ=255)              gCCCTGGCTGCTGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAACTTGGTGAGGCGCTGGCTCGGGCGGTCCTAAAGCCTGCACACCCTGCTCGTGCACGTCTTTCGTTTGATTAACGGCCAAGGTATCAGAACTCCACCAACTGATGCTGTTCATTAAGACGATCGCGGACTCCGGCTCGAATATTTTTTTCACCTTCACTGGCGACGGCGAAGCGAACCTTTTCTCCGTCATGATATg                                                                                                                                                                                                                               >  1:252541/1‑251 (MQ=255)                             cGACCAGCCTTTCGTTCAGGGAATTACGGCATGGGCCAACATTGGTGCGGCGATGGCTCTGGCGGTCGTTCAGCCCGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTTAAGGTAAAGCTGTCAGCACTCCACAACCCGATTCTGCTCCTTACACCGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGataagaacggaagagcaaac                                                                                                                                                                                                                >  1:281108/1‑234 (MQ=255)                              gACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTTCATCCCGTGCTCGTCGGCGTCTTTCGTTTTAGTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTTTTCATTAAGACGAGAGCTGAGTCTGGCTCGAAGATTGTTTGCACCTTCAATTTCGGCGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTtcc                                                                                                                                                                                                               <  1:185822/251‑1 (MQ=255)                                              aGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGAGTCTTTCGTTTGATTAACGGTAAAGCTATCAGCACCCAACAAAATGATGCTGTTCATTAAGACGATAGCGGAATCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCATGGACCGAGaat                                                                                                                                                                                                >  1:137429/1‑250 (MQ=255)                                                                                gATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTCAAGCTATCAGAACTCAACAAACTGATGCTGTTCATTAAGAAGATAGCGGACTCCGGCTCGAATATTGTTTTCAACTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGCCATGat                                                                                                                                                                                                                                  >  1:191361/1‑182 (MQ=255)                                                                                        gggCGGTCGTAATGCCTGCGTCCCTTGCGCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTAGCAGCACTTAACAAACGGATGGTGGGCGGTAAGACGGTAGCGGAGGCGGGCTTTAATGTTGTTTTCCCCTTTAATCGGGACGGCGTAGCGAACTTGTTCGAGGTCATGAGATTGCAGGGCTGCGTTTGTGCCTTGCTTGAGAATAATTGTGTCGAGCACGCCCTTGCTTACTACCGTTGAATTTTCa                                                                                                                                                     <  1:316390/251‑1 (MQ=255)                                                                                         gtcGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCCGCCCTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAc                                                                                                                                                    >  1:328376/3‑251 (MQ=255)                                                                                           nggTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCg                                                                                                                                                  >  1:63783/2‑251 (MQ=255)                                                                                                gTAAANNATNNNTCNNGTGCTCGTCGGCGTCTTTCGTTTNATTAAAGGTAACGCTATCAGCACTCAACANANTNATGCNNTTCNTTAAGACGATNGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAANCNAACTTTTTCGCCGTCATGATATGCCAGTGCGNAGCTTCCTCCTGGCCCGAGAATAANTGaan                                                                                                                                                                                        >  2:268481/1‑206 (MQ=255)                                                                                                       cTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGCAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGAATCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATACTTAAGTCCACCACCCCCCTGCTCACTACCGTTGAATTTCCACCGCTGGc                                                                                                                                             >  1:176204/1‑244 (MQ=255)                                                                                                                                                       aTCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCTACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCGTCCTCCTGGCTCGAGAATAATTGAGTCTACCACGCCCGTGCTCACTACCGGTGAATTTTCACCGCTTGTAATGATGTGTACAGTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTg                                                                                      <  1:346842/251‑1 (MQ=255)                                                                                                                                                                   caaACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCAcc                                                                          <  1:107752/251‑1 (MQ=255)                                                                                                                                                                                   aTTAAGACGATAGCGGACTCCGGCTCGAATAGTGTTTTCACCTGCAATGGCGACGGCTAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATATGTACATTGTCGCCGCTTTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTgg                                                          <  1:268481/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTcgn                                                                                                                         <  1:41785/140‑2 (MQ=255)                                                                                                                                                                                                                                    gCGACGGCGAAGCGAACTTTTTCGCCGTCATGATACGCCAGTGCGGCGCTTCATCCTGGCCCGAGAATATTTGAGTCGACAACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGAACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCggg         >  1:334592/1‑250 (MQ=255)                                                                                                                                                                                                                                        cGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGCGTCGACCACGCCAGTCCTCACTACCGTTGCATTTTCACCGCTGGCAATGATGTGTACCTTGTcgcc                                                                                                                        >  1:303933/1‑136 (MQ=255)                                                                                                                                                                                                                                            gaagcgaaCTTTTTCGCCGTCATGATATGCCAGTGCGGCGATTCCTCCTGGCACGAGAATCATTGAGTCGACAACGCCCGTGCTCACTACCGTTGAATTTTCACCGACGGCCATGATGTGTACATTGTCGCCGCTGTTGCCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCACTGTACAAACGCGTACTCGCATTCCGGTATTCGCa  >  1:245212/1‑250 (MQ=255)                                                                                                                                                                                                                                              agcgaaCTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTGCCTCCTGGCCCGAGAATAATTGAGTCGACCACTCCCGTGCGCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGc                                                                                                               <  1:176554/139‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                CGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCA  >  NC_000913/1748596‑1749080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑