Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	292,019	C→T	L174L (CTA→TTA)	yagJ →	protein YagJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	292,019	0	C	T	100.0%	16.3 / NA	7	L174L (CTA→TTA)	yagJ	protein YagJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/2);  total (5/2)

GAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGC  >  NC_000913/291814‑292246                                                                                                                                                                                                              |                                                                                                                                                                                                                                    gAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTCGCTTTCTGGTCTCGTACTCCACAGTACCCAGGGCCGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAGCAGAACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAATTGGTTGTGGACCTGGAAGATCCACATCGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCtat                                                                                                                                                                                        >  1:55788/1‑251 (MQ=255)     cATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACCGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAACCTGGAAGGACTTGCGCTATATGc                                                                                                                                                                                    >  1:248889/1‑251 (MQ=255)           ggaaCCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAAACGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAAc                                                                                                                                                                               >  1:338993/3‑250 (MQ=255)                    gTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTACTCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTg                                                                                                                                                                      >  1:322924/1‑250 (MQ=255)                                                                                          cgtcACTGGGAATCGCTTATGGTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAAGAGGATGGCAAAATGTGTGTGGACCTGGAAGATCCGCATAGCTTACATCTGGCGGTTGCTTTGCCCAAACTGTAAGGACTGTCTCTATATGCCGAACATCATTCTGAGTCTTACAGGTGAATCGAATCTGTCGCCGAAGTAAAGGGTAAAGTACGAGTGTTAGATTTGAAACGGCAg                                                                                               <  1:350126/251‑1 (MQ=255)                                                                                                                                 aGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATACACATAGCTTACATCTGGCTGATACTTTGCCCACACTGGAAGGACTTGCGCTATATACCGAACATCATTCTGATGATTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAGTGCAg                                                          >  1:178913/1‑249 (MQ=255)                                                                                                                                                                                        ggACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGc  <  1:111387/250‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                    GAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGC  >  NC_000913/291814‑292246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑