Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,460	G→A	Q369* (CAG→TAG)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,460	0	G	A	100.0%	32.5 / NA	13	Q369* (CAG→TAG)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/9);  total (4/9)

ACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAG  >  NC_000913/1667221‑1667704                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     aCTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTATAAGTTACGCTTTCCTGGCCAAAATTGAGATAGCGCAACTTTTGGTAGAAGAGTGAAAAAATGGTAACACTGCACAAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTATAAACGCTGATGTGCTAACTATACGc                                                                                                                                                                                                                                             >  1:48130/1‑249 (MQ=255)        ccACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGGTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGc                                                                                                                                                                                                                                    <  1:174649/251‑1 (MQ=255)                    ttCACAAAGATAAACCAGGAAAATAAGTAACCTTGAAAGTCTAAGGTATGCTTTCCTGGCCCAAAGTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAAGAAAGAACCGTTATGCATCGCGGCGTGTGCCAGTGCATCGCCTGCCATCGTGCCCTGGTTAGAAAACTTAGTTCTCTCAACGCTTATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGa                                                                                                                                                                                                                         <  1:190368/250‑1 (MQ=255)                                                                        ttCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGTCCTGCTGACGGATTCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGtn                                                                                                                                                                      <  1:64104/249‑2 (MQ=255)                                                                          ccTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGGGCTAACTATACGCAGGAAGGGCCGGCGGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGc                                                                                                                                                                   <  1:207395/250‑1 (MQ=255)                                                                                ccAAATTGAGAAAGCGCAAATTTTGGTAGAACAGTTAACCAATGTTAACCCTGCCACAGACGACTCAACCCAGCACCGTTATATATCGCGTCTTTTACCCGTGCAGCGCCTGCCATCGTGCCCTGGTTAGCAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCCGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGAGAGCCAATc                                                                                                                                                              >  1:260643/1‑249 (MQ=255)                                                                                             gcgcAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATAcn                                                                                                                                                                                                                                              <  1:216962/156‑2 (MQ=255)                                                                                                cAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCtn                                                                                                                                                                                        <  1:85226/207‑2 (MQ=255)                                                                                                            gAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGa                                                                                                                                                                                                    >  1:112435/1‑183 (MQ=255)                                                                                                                                            cGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAGTTTGTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGcc                                                                                                <  1:133644/251‑1 (MQ=255)                                                                                                                                                                                  cAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCGCAACGCTGATGTGCTAACTATACGCAGTAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAGCAGTATTTTTTGGGGGTTAAATAAATTCACCAGGATGGCATGAATGCGCCCGACATGCGGACCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccgc                                                          <  1:331726/251‑1 (MQ=255)                                                                                                                                                                                                                 gAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcg                                                                                                    >  1:94767/1‑178 (MQ=255)                                                                                                                                                                                                                                           gtgCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTATTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCTCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAg  <  1:345522/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAG  >  NC_000913/1667221‑1667704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑