Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,679	A→T	L209Q (CTG→CAG)	ydfU ←	protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,679	0	A	T	100.0%	22.4 / NA	10	L209Q (CTG→CAG)	ydfU	protein YdfU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/8);  total (2/8)

CCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTC  >  NC_000913/1643512‑1643866                                                                                                                                                                        |                                                                                                                                                                                            ccTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTcc                                                                                                                                                                                      <  1:201587/175‑1 (MQ=255)  cTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGc                                                                                                                                                                        <  1:61889/188‑1 (MQ=255)             ggcgACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCCCCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAgg                                                                                                     >  1:155996/1‑244 (MQ=255)                                                                                 ttttCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAATGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGTGTTCGCGGTACACCGAGCAGATTGTTTCCGCTGGTAATCCCAGCGAACGACGCTATTCAGCCTCATGTAGTGCATc                                                                                            <  1:295847/185‑1 (MQ=255)                                                                                 ttttCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACTGTGCAAGATTTTTTGTGCGGTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTGTcgcg                                                                                                                                                                 <  1:287839/116‑1 (MQ=255)                                                                                          ggtggtTTTTGTTGCTGGGGGGCGGAAGGCAACGGTGCAAGATTTTTTGTGCTTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCGTTCGCGGTCCACCGAGCAGATTTTTTCCGCTGGTAAGCCCAGCGAACGACGCGATACAGCGTCAGGTAGTGCATCCGCCACCTGATTGCAGACCGCTCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTg                  <  1:184828/250‑1 (MQ=255)                                                                                                          ggtggGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGAGTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTc  <  1:293129/250‑1 (MQ=255)                                                                                                                                           tgCGTTGTTTCAATATGCTGGTGGCGNTCTGCTNNCCNGGTACGATGTCGCTTTCGCNGTNNNCNNNGNCGATTNTTNNCGCTGGTAANCCCAGCGAACGACGCGATACAGCCTCAGGTAGTg                                                                                                >  2:77552/1‑123 (MQ=255)                                                                                                                                           tgCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTg                                                                                                <  1:77552/123‑1 (MQ=255)                                                                                                                                            gCGTTGTTTCAATATGCTGGGGGCCGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGGACACCGAGCAGATTTGTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATccacc                                                                                        <  1:204391/130‑1 (MQ=255)                                                                                                                                                                        |                                                                                                                                                                                            CCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTC  >  NC_000913/1643512‑1643866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑