Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,247	C→T	I11I (ATC→ATT)	ydfZ →	putative selenoprotein YdfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,247	0	C	T	95.5%	54.5 / NA	23	I11I (ATC→ATT)	ydfZ	putative selenoprotein YdfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/9);  minor base G (0/1);  total (13/10)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATT  >  NC_000913/1629006‑1629469                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               cAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCACAAAAGATGTGAGCTTGATCAAAACCAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGc                                                                                                                                                                                                                       >  1:343629/1‑251 (MQ=255)                         gAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGGGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCGTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGGAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCAcn                                                                                                                                                                                               <  1:89276/251‑2 (MQ=255)                            gagTAAGTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACTATCGGAACCGTAACGCAATTACCACTGGCAGCCg                                                                                                                                                                                                                  <  1:120527/229‑1 (MQ=255)                                          ntctcATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGAATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAACATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGAACCGGTCACACTGGCa                                                                                                                                                                                  >  1:65273/2‑247 (MQ=255)                                              catcccatccGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATTCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTGATATTGCGCCCGTTACGTGGGCGTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATGACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATc                                                                                                                                                                             <  1:53292/248‑1 (MQ=255)                                                               nagTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAACGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAgg                                                                                                                                                         >  1:39430/2‑251 (MQ=255)                                                                          cccGCCTTATGGCTCATTCATGCATGAAAAAAGATGTTAGGTGGATCAAAAACAAAAATTATGTCACTCTACTGGATGATTTATATGGCGCCCGTTACGGGTGCTTGGAATGTGAATCAGAAGGGAGAAAACACCTATGACGGCTTTCGGGCGTGACTGTGACGCAATTACCACTGGGAGCCGTGGTAGGGTTAGCGTGGCCGGTCGCACTGTAAAGAGCCTGTCGATTGATACTGAAGGTCTGACCGCTg                                                                                                                                              <  1:335137/251‑1 (MQ=255)                                                                                    ggCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTCTATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccg                                                                                                                                    >  1:274285/1‑251 (MQ=255)                                                                                                   cAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGCCAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTCCGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGaa                                                                                                                            >  1:166492/1‑244 (MQ=255)                                                                                                     aaaaaGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCgg                                                                                                                                                                                             >  1:18146/1‑177 (MQ=255)                                                                                                                aGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTAGATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGATAAAACACCTATGACGTCCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGc                                                                                                                                           <  1:315654/216‑1 (MQ=255)                                                                                                                 gCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTGTGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGtt                                                                                                       >  1:15294/1‑251 (MQ=255)                                                                                                                 gCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGtt                                                                                                       >  1:260592/1‑251 (MQ=255)                                                                                                                    tGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTNGCANNNnngtnn                                                                                                                                                                                                             >  2:211413/1‑144 (MQ=255)                                                                                                                    tGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACATTGGCAAGATCCTGTCGATTgn                                                                                                                                                                  <  1:211413/189‑2 (MQ=255)                                                                                                                      aTCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACAACATACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGTCAAGATCCTGTCGATTGATACTGATGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGaa                                                                                                  <  1:1961/251‑1 (MQ=255)                                                                                                                                 aaaaTATTGCACTCGACAGGAGTATTTATATTGCGCCCGTTTCGTGGGCGTCAACTGTAAAGCAGTAAGGAGAAAACACCTATGCCGACCTACGACCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTGATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCTATGGATACTGAATGGCGGACCGCTGAGCAAAGCCGCCGCGGAAAAGCCGTAGTTGTAGAAGGTTGTGAAGAGAAACTGGc                                                                                       <  1:147824/251‑1 (MQ=255)                                                                                                                                          naCTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCa                                                                                                                                                                                                 >  1:72096/2‑136 (MQ=255)                                                                                                                                                                                   tNNACTGTANNTCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCgg                                                                                                                                                                                             <  2:18146/99‑1 (MQ=255)                                                                                                                                                                                           annTCAGAANNGAGAAAACACATATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGTCAGCCGCGTTATGGTTAGCGGCCCCGGGCACGCTg                                                                                                                                                                                     <  2:15590/99‑1 (MQ=255)                                                                                                                                                                                               cAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCACTTACCACTGGCCGCCGTGTTATGGATAGCGGCACCGCACACACTGTCAAGATCCTGTCGCTTGATACTGAAGGTCTGACCGCTGAGCAACTCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGCGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGCATGCCGCCGATGGCGGCAtt                         >  1:318525/1‑251 (MQ=255)                                                                                                                                                                                                                    nacgacCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTc                                                                                                                                                       >  1:62196/2‑104 (MQ=255)                                                                                                                                                                                                                      ngacCTACGATCGTAACCGTAACGCAATAACAACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATAtt  >  1:104781/2‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               CAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATT  >  NC_000913/1629006‑1629469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑