| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,541,058 | G→A | T511T (ACC→ACT) | narZ ← | nitrate reductase Z subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,541,058 | 0 | G | A | 90.0% | 20.9 / ‑5.9 | 10 | T511T (ACC→ACT) | narZ | nitrate reductase Z subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (2/7); total (2/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGC > NC_000913/1540834‑1541260 | tgtgGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGATCGCCCATGCTTTTTATGGGCGGTATCGGCAAATTCACGGTCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTgggg < 1:345974/250‑1 (MQ=255) ttGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCAGCGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTAACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGcc > 1:173051/1‑251 (MQ=255) gcAGCATAGTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACTGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGGGGCCGTAATTCGcc < 1:45375/250‑1 (MQ=255) cAGCATATTGATCATCCCGCGGTAGTTCATGTACATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAAGTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGcc < 1:27019/250‑1 (MQ=255) atatTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCGCTCCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTAc < 1:174838/251‑1 (MQ=255) gTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCTTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGan < 1:92763/251‑2 (MQ=255) agggtAATCATAGAGCGCCCATGCGGTGTATGGGCGGGAGCGGCAAATTCACGGGCGATGGTTTCGATAGAGGGGCGCGGAACGCCAGTAATTTGCGCACCTCAGGATGGGGGGTACGGTTGGATTTCAGCGTAAGCGTTCGCAAGGTTTTCATCTGCCAGCCCGCGATGGAGGCCGTAATGCGCCAGTGCGAAATCATAAACGCTGACCACCGGACAGGTGGTGCCATCGACTAACGTCAGGTTTTTAAc < 1:207482/247‑1 (MQ=255) gggggTATCGGCAAATTCACGGGCGATGGTTTCGATATTCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTGCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTc < 1:166300/148‑1 (MQ=255) aTCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTTTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGTCGCACCAGCACCGGATTGTGTTTTACGc < 1:301841/251‑1 (MQ=255) gcgGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTATTCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATc > 1:303037/1‑94 (MQ=255) | TGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGC > NC_000913/1540834‑1541260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |