Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	100.0%	18.8 / NA	10	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/5);  total (5/5)

CCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAA  >  NC_000913/284296‑284777                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ccGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAAGGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTc                                                                                                                                                                                                                                         <  1:2303/251‑1 (MQ=255)            gCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAgg                                                                                                                                                                                                                              <  1:118308/251‑1 (MQ=255)                             tCGGCCTGCTGCTTCTGGACGCCATGACCGCGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCAGCGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCAGGAGAAGGCAACAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGAAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCg                                                                                                                                                                                                             >  1:295814/1‑251 (MQ=255)                             tCGGCCTGCTGCATCTGGACGCAATGAACGTGACAGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCg                                                                                                                                                                                                             >  1:97385/1‑251 (MQ=255)                                             ggACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGGGGGCGa                                                                                                                                                                                               >  1:145463/1‑249 (MQ=255)                                                                                    ccTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAGGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACGCCGGCCGGGTGCGGGTGTTTg                                                                                                                                                      <  1:115210/251‑1 (MQ=255)                                                                                                    gCGTCCGTGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGGGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCGCGACGGTCTGCGTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggc                                                                                                                                      <  1:242611/251‑1 (MQ=255)                                                                                                                             gCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGAAAAAGAGAAAGGGCTGAACTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCAGGACGGTTCGGCGATCAAGGCAACGGCGATCGAcc                                                                                                                                                                                                               >  1:236305/1‑153 (MQ=255)                                                                                                                                                                                                                                   cGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGGGAAGATGGCGAATGCCACCACACCGGCCGGGTGCGGGTGTTTGTCGCGGAAGCGCATGCTATCAAGGCGATCAAGCGGGAAGAGATTTTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTTCGGCACCGGCATGGAag                                                <  1:196198/210‑1 (MQ=255)                                                                                                                                                                                                                                         aCATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGAGAAGATGGCGCATAACACAACACCGGCCGGGTGCGGGTGTTTGTCTAGGAAGCGAAGGCGATCAAGGCGATCAAGCGGGAAGCGATTGTGAAGGGCGATATCATGGTGGTGATCGGCGGCGGGACGTCCGGCACCGGCATGGACGAGACCAACCAGCTCCCCTCCGCGCGAAAGCATATCTCGTggggaaa  >  1:256383/1‑247 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   CCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAA  >  NC_000913/284296‑284777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑