Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,522,583	C→T	Q161Q (CAG→CAA)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,522,583	0	C	T	100.0%	35.0 / NA	13	Q161Q (CAG→CAA)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

GCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATC  >  NC_000913/1522404‑1522791                                                                                                                                                                                    |                                                                                                                                                                                                                 ncTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTtgt                                                                                                                                                                                                               >  1:91376/2‑183 (MQ=255)         tttCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGAAAAACGCGTGGTTGAGACGATGTAATCGACATCGACAGGCTGAGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGAGCCAGCTGCCAAAACTGCCGTAGTAAATACTGGCTTCAATGGTTGGGGGttgttg                                                                                                                                                                                                             >  1:182024/1‑177 (MQ=255)                               cGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGttgttg                                                                                                                                                                                                             >  1:233462/1‑155 (MQ=255)                                                            tGAGACGGTGTAATTGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGTATGTttgcccttgc                                                                                 <  1:119065/250‑1 (MQ=255)                                                                          cGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGTTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAgggg                                                                                                                                             <  1:290645/176‑1 (MQ=255)                                                                                                                            ccATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCCCCAGACGCGTTGCCATACAGGGCAGAGAAGGGGc                                                                                                                                            >  1:51494/1‑127 (MQ=255)                                                                                                                              aTAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACGGGCGTCAATGGTTGGTGGTTTTTGTCCGGGCTGGGTGGTGACATTCAGTACCCCACGAGACGCGTTGCCATACAGGGCAGAGAAGTGTCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTttgcccttgc                                                                                 <  1:248610/184‑1 (MQ=255)                                                                                                                              aTAGCGCANGNNNCNNAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGNCCGGTCTGGGTGGTGNCANCCATNACCNCAAAAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACCTTTTGCCCACTGCTTAGATCGACGCTGGATGTttgcccttgc                                                                                 >  2:248610/1‑184 (MQ=255)                                                                                                                                     cAGCTGCCAAAAATGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGCGAAGGGGCCACGCAGCACTTCCANATTNNNnncann                                                                                                                >  2:210264/1‑144 (MQ=255)                                                                                                                                          gCCAAAACTGCCGTAGTAACTACTGGCTTCAATTGTTTGTTGTTTTTGTCCGTTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGCCAGAGAAGGGGCCACGCATCACTTCCGCATATTGCACACTTCTTAAGTCGAGGTTGGAGGTTTGTCCTTGCCCGTCTGGCGTTGAGGCGGGAATACCGGCCACATACAGGGTAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAAt   <  1:341661/250‑1 (MQ=255)                                                                                                                                          gCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATNCAGNGCAGNGANGGGGCCACGCAGCACNTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCNNGNNNGNCGGGCAtg                                                                     <  2:245071/184‑1 (MQ=255)                                                                                                                                          gCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATc  >  1:13189/1‑251 (MQ=255)                                                                                                                                          gCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCAGGTCTGGGTGGCGACATTCATTACCCCACCACACGCGTTGCCATACAGGACAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCCCACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCAtg                                                                     >  1:245071/1‑184 (MQ=255)                                                                                                                                          gCCAAAACTGCAGTAGTAACTAATGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGACATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATc  >  1:302518/1‑251 (MQ=255)                                                                                                                                            naaaaCTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATc                                                                                                       >  1:72502/2‑148 (MQ=255)                                                                                                                                                                                    |                                                                                                                                                                                                                 GCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATC  >  NC_000913/1522404‑1522791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑