Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,395,367	G→A	R186C (CGT→TGT)	ynaI ←	small conductance mechanosensitive channel YnaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,395,367	0	G	A	100.0%	24.4 / NA	12	R186C (CGT→TGT)	ynaI	small conductance mechanosensitive channel YnaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

CCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAA  >  NC_000913/1395168‑1395606                                                                                                                                                                                                        |                                                                                                                                                                                                                                                cccACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCgg                                                                                                                                                                                               >  1:143643/1‑250 (MQ=255)                                                         ggTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTACGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATac                                                                                                                                      >  1:267994/1‑251 (MQ=255)                                                                             cTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTCATTTTGGTAATTCGACAGCCAATTTCCGCTACTGTACCTTCGATATTTATGTACGGAGAACAGATCCAGTCGACAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGcc                                                                                                                   >  1:338408/1‑250 (MQ=255)                                                                               aCGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAGTGGACGATTATAAAAGGCAGTAATTTTGGTAATTCGCCAGCCAATTTACGCTACTGTACATTCGATATTTCTGTCCGGTGAACAGATACAGTCGCCAATACTGAAAGGAGGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCg                                                                                                                                                                  >  1:232076/1‑201 (MQ=255)                                                                                    gATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAg                                                                                                                                                     >  1:119264/1‑209 (MQ=255)                                                                                            aaaaCAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGAtn                                                                                                                                                                                                                                             <  1:72926/113‑2 (MQ=255)                                                                                                                               tCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCGGTCCGGTGAACAGAGCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGcac                                                                      <  1:192237/245‑1 (MQ=255)                                                                                                                                             nttNNTAATNCGACAGCCAATTTCCGCTACTGTCNCTTCGATATTTCTGTCCGGGGAACAGATCCANTNGCCAATACTGAAAGGACGGTCGAAATAGAGCATAACCCCGGAAAAGAANTTACTCAGAANAGCNNTACCNGNCNTACCGACAGCCAGACCACCAATACCACCANAGGTCAGCAAGCCAGAAAGGCTCNNTCNNNAANNTTCGc                                                                                         <  2:267994/211‑1 (MQ=255)                                                                                                                                                 ntAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAata                                                                                                                                                                           >  1:34028/2‑126 (MQ=255)                                                                                                                                                  tAATTCGCNANNNANNTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCNGTCGCCAATACTGAANGGNAGGTNGAANTAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGcc                                                                                                                   >  2:242448/1‑181 (MQ=255)                                                                                                                                                  tAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAAGACTGAAAGTACGGTCGAAATAGAGCATAATCCCGGAAAATAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGcc                                                                                                                   <  1:242448/181‑1 (MQ=255)                                                                                                                                                                                             gTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCGTTACCGGCCATACCGACAGCCAGACCACCAATTCCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTaa  <  1:153087/251‑1 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                                CCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAA  >  NC_000913/1395168‑1395606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑