Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,684	T→C	intergenic (‑39/‑104)	clsA ← / → yciY	cardiolipin synthase A/uncharacterized protein YciY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,684	0	T	C	100.0%	48.1 / NA	18	intergenic (‑39/‑104)	clsA/yciY	cardiolipin synthase A/uncharacterized protein YciY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/6);  total (12/6)

CCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTTACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGT  >  NC_000913/1308470‑1308928                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ccAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGCACTGCGCGTCGTTTCATTCGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGCATGGCCACCCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGCTTCATCTGGCAACTAAAAACGCggg                                                                                                                                                                                                                  >  1:254873/1‑251 (MQ=255)                                                            ggCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAAAATGCGTAAAGTTACCAATGAAATGAGCAACCAGTATCCCAGAATGGACTACCACCTCACCAACGTATAAACGGTTGACATCGATTCGCAATCCTTTTGAAAGCGCATTGTTTTGAGTTCACGCATATGGATTAATCTGGCAACTAAAAACGCGGGAACAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATag                                                                                                                                                         >  1:347784/1‑249 (MQ=255)                                                             nccATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTAATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGGGGGGGTATAATGACCATTCTGTTATTGCATAGagt                                                                                                                                                      >  1:216106/2‑251 (MQ=255)                                                             gCCACCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGACCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTTTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGTGTATAATGACCATTCTGTTATTGCATAGagt                                                                                                                                                      <  1:129715/251‑1 (MQ=255)                                                                     ggAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGGATAAACGGTTGTCATAGATTAGAAATCCTTGTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAAGAAAGACGCTGGAAAAGCACAGGTCAGCGTCggg                                                                                                                                                                                            <  1:106239/205‑1 (MQ=255)                                                                                            gtAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTGTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTcn                                                                                                                                                                                              <  1:37611/179‑2 (MQ=255)                                                                                                                   ncAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTa                                                                                                                                  >  1:66253/2‑217 (MQ=255)                                                                                                                               cAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACg                                                                                    >  1:47955/1‑251 (MQ=255)                                                                                                                                      ccAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGCATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATcg                                                                             >  1:354365/1‑251 (MQ=255)                                                                                                                                           aTGGCCAACCACCTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGATATGAGTTCACGCATCTGGATTCATCTGGCACATAAAAACGCGGGAAAAGCACTGGTCAGc                                                                                                                                                                                                  >  1:51844/1‑129 (MQ=255)                                                                                                                                              gCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCt                                                                     >  1:292451/1‑251 (MQ=255)                                                                                                                                               ncaaccaacTCACCAAAGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCt                                                                                            >  1:86860/2‑227 (MQ=255)                                                                                                                                                naaccaacTCACCAACGTATAAACGGTTGTCATAGATTAGACATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGAc                                                                                     >  1:31114/2‑233 (MQ=255)                                                                                                                                                 aaccaaccCCCCAACGGATAAACGGTTGTAATAGATTAGAAATCCTTTTGAAAGCGCATTTTTATGAGTTCACTCAGATGGATTCATCTTGCAAATAAAAACACGGTAAAATCACTGGTCAGCGTCGTGGGTGTGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGATTCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGa                                                                  <  1:350786/251‑1 (MQ=255)                                                                                                                                                            cAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGTTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCTGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATcgc                                                       <  1:204039/251‑1 (MQ=255)                                                                                                                                                                                            ccTTTNNAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCgggggtgggggt                                                                                                                                                                                   <  2:28781/95‑1 (MQ=255)                                                                                                                                                                                                   aaaGCGCCTTGTTATGAGTTCACGCATATGGATTACTCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATACAGCGTCAGGCTAGCCGACGTAAATCGCGt                                                                          >  1:280009/1‑193 (MQ=255)                                                                                                                                                                                                                 ngAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGAATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGAGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATAGCGCCGAAATATGAGTATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGt  >  1:32466/2‑251 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTTACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGT  >  NC_000913/1308470‑1308928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑