Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	32.0 / NA	12	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/7);  total (5/7)

GTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATC  >  NC_000913/1169677‑1170060                                                                                                                                                                |                                                                                                                                                                                                                                 gTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGTCAACTCAATTCCCTGTGCTTTATATCGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGc                                                                                                                                          <  1:321159/248‑1 (MQ=255)               gagTGTTTTGATATCGTCATCCCGCAGACGAATACAGACAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTAGTACCCTGAAGCAAATAACAGCCGCCATAGGCCGCCAGACGAATCGCATGATGGACCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGaaaagg                                                                                                                         >  1:303835/1‑248 (MQ=255)                                      gCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGGACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCan                                                                                                                                                                                                                     <  1:67778/136‑2 (MQ=255)                                           cGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTANCGCNNNNNAcnnnnnnnnnn                                                                                                                                                                                              >  2:222794/1‑144 (MQ=255)                                                    ncAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTa                                                                                     >  1:42879/2‑250 (MQ=255)                                                                                               cATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTAccn                                         <  1:222794/251‑2 (MQ=255)                                                                                                              cgccgcCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGAAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTGGGCGTCCATGTTGGGTTTGCTCGTTTGTCTGAAACGGTGGTCTCCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATTCCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATag                          <  1:15335/251‑1 (MQ=255)                                                                                                                  gccATAGTCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAGCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTTTGCTTTAGAACGGGCTCGGATGTTTGCCGGTTGCTTCCATGTTGGTTTTGCTCGGTTGTCTGAGACGGTGGTCACCAGGTTCGGTGTCAGCGTGTCACCACCGAACTTACCAATACCTATTGGATACACGGt                                                         <  1:341494/216‑1 (MQ=255)                                                                                                                  gccATAGGCCGCCAGACGAATCGCATGATGGCCCCTTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAgg                                                                                                                                                    >  1:302823/1‑125 (MQ=255)                                                                                                                   ncATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCcggcggatctgatgctcacct                     >  1:102952/2‑236 (MQ=255)                                                                                                                   ccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGGTGTTTGCCGTGGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGGGTCAGCGGGTCACCACCTAACTGTCCAATCCCTAGTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACg                     <  1:351596/251‑1 (MQ=255)                                                                                                                                       cGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACGAGCGCATGCAACGCAATTCCCTGTGCTTTATAACGGGCGCGGATGTGTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACTGTGGTCACCATTGTCGGTGTCAGCGTGGCTCGTCCTAACGTACCAATTCCTATTGGATACACGTTTACCGAATTTTTTCCCTGCGGGTAGTAATAGAGACGCAGCTCCTCAATTTTGATc  <  1:56527/250‑1 (MQ=255)                                                                                                                                                                |                                                                                                                                                                                                                                 GTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATC  >  NC_000913/1169677‑1170060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑