Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,792	T→A	S58T (TCG→ACG)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,792	0	T	A	100.0%	32.4 / NA	14	S58T (TCG→ACG)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (4/10);  total (4/10)

ACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGG  >  NC_000913/1147602‑1148005                                                                                                                                                                                               |                                                                                                                                                                                                                      aCCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCGTCCGTGACAGTGCCTGCAGCAGTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTGTTAGTCGGCAATTCCGACTCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACTCGTCTGCAGATTATTCCTGCTCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAg                                                                                                                                                           <  1:264451/251‑1 (MQ=255)           gacgaTACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGc                                                                                                                                                          >  1:21766/1‑242 (MQ=255)                                                            aTTTTGGCCCTTCCGTGACAGGGCCTCCAGCATTGCAGGCACTGAATTCTAAGTCGCAACTCCCTCTTCTTTTAGTCGGCAATTCCGACACCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCACAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCTTGGc                                                                                                                      <  1:45194/229‑1 (MQ=255)                                                                            gacagttccgtcatcaTTGCCGGCACTGAATTCTAATTCGCAACTCACTTTTCTTTTAGTCGGCAATTCCGCCGCCATCACGCCATTACTTGCTAAAGCTGCCTTTGAACAACGTACGCGTCTGCAGATTATTCCTGTGCAGTCAGTGATCTCCAGTGATGCCCGGCCTTCTCATGCTACCCGCGCCAGCCTTGGTAGTTCAATGCGCGTTGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCtgtg                                                                                <  1:239224/237‑1 (MQ=255)                                                                                  gCCTGCAGCATGGCAGGCACTGAATTCTAATTCGCAACTCCCTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGCCAGTTATCGCCAGTGa                                                                                                                                                                            <  1:189525/153‑1 (MQ=255)                                                                                     tGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGCCTTTGAACAACGTACGCGTCTGCAGATTATTACTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCcgcg                                                                                                                                                  >  1:47176/1‑176 (MQ=255)                                                                                                              aaTTCGCAACTCACTCTTCTTTGAGTCGGCAATGCCGACGCCATCACGCCATTACTTGCTAAAGCTGACGTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATTCGCTGGGCCCGGGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATgggg                                                <  1:255423/249‑1 (MQ=255)                                                                                                              aaTTCGCAACTCACTCTTCTTGTAGTCGGCAATCCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTTGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCGGTGTCAGTGCCGGTAATACCGGGGCGCTGATggg                                                 <  1:137723/248‑1 (MQ=255)                                                                                                                    cAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCCTCACGCCATTACTTGCTACAGCTGACTTTGAACAACGTAAGCGTCTGCAGCTTATTCCTGCGCAGTCAGTTATCGCCAg                                                                                                                                                                               >  1:236525/1‑116 (MQ=255)                                                                                                                                            aaTTCCGACGCCATCACGCCATTACTTGATAAAGCTGCCTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAAGGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCGGTGTCAGTGCCGGTAATACCGGGGCGCGGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAggg                <  1:149391/251‑1 (MQ=255)                                                                                                                                                          cACGCCATTACTCGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTGCGCAAGCTATCCGCGCCAGACGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTTGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCgg  <  1:240853/251‑1 (MQ=255)                                                                                                                                                            ngCCATTNNTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGc                                                                                                                                                          <  2:21766/96‑1 (MQ=255)                                                                                                                                                                     ttGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTg                                                                                                         <  1:10981/137‑1 (MQ=255)                                                                                                                                                                     ttGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCNNTCcgcgnn                                                                                                                                                >  2:10981/1‑96 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                                                      ACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGG  >  NC_000913/1147602‑1148005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑