Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,068	A→G	V45A (GTA→GCA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,068	0	A	G	100.0%	41.6 / NA	15	V45A (GTA→GCA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/7);  total (8/7)

CCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTC  >  NC_000913/155850‑156272                                                                                                                                                                                                                           |                                                                                                                                                                                                             ccAGTACGTTAAACCAGAACACGCTCTCTCTGGCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAATCAACTCTGGACAAGTAACGTGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATAtt                                                                                                                                                                              <  1:310743/251‑1 (MQ=255)              ccAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTCATTTGCTTGTTAACCCACGTTTGGAATCAATAAGCGATACTGGCGGCGTAGCCGTAAAAGGGACTGTACTACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTACCCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCg                                                                                                                                                                 >  1:331625/1‑251 (MQ=255)                   acacGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGAAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATg                                                                                                                                                            <  1:95037/251‑1 (MQ=255)                                          aGTGAGGTGCTTGCTGTGTACATTAATTTGATTGTTTGCCCCCGTGTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATCCTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACCCTTTTTTGATCGATTTTATATATTACGCGAGTACTCGAAATGACAATGTCCGCAATAGACGATNa                                                                                                                                     <  1:275997/251‑1 (MQ=255)                                                              naTTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGtt                                                                                                                  >  1:38024/2‑250 (MQ=255)                                                               aTTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACGGCCAGGCTCAGCGTTGTCATCGCCAGGATCTAACCAATTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGaca                                                                                                                                                                                                      <  1:173320/165‑1 (MQ=255)                                                                   aTTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAg                                                                                                            <  1:140371/251‑1 (MQ=255)                                                                                                         aCTGGCGGCGTAGAAGAAAAAGGGACTGTAATACTGCCAGGCTCAGCGATGTCATAGCCCGTATCTAAACAACTCTGGACAAGCAACGGGTTATTAACTTTATTTTTCAGACGTGCGTTGCCACTTTTTTGATCCCTTTTATATCTTACGCGAGTACCCGACAAGACAATGTCCGCAATAGACGATGAAATAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTCTGTTTGGTGTTAAAAAACATaa                                                                       >  1:236806/1‑250 (MQ=255)                                                                                                            ggcggcGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGt                                                                                                                   >  1:99745/1‑203 (MQ=255)                                                                                                                                     tAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACa                                                                                                                                                         >  1:350134/1‑140 (MQ=255)                                                                                                                                      aaTACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAgg                                         >  1:5778/1‑251 (MQ=255)                                                                                                                                                                             ncAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATattc  >  1:65987/2‑251 (MQ=255)                                                                                                                                                                                 ctcgggACAATCAACGGGTTATTCCCTTTATGTTGCAGACGTGCTTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTAccc                                                                                                                                                                  <  1:50696/83‑1 (MQ=255)                                                                                                                                                                                    tGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAg                                                                                                                             >  1:19322/1‑121 (MQ=255)                                                                                                                                                                                                  tttATTCCCGTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCGTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGc                                                                                                                                                 <  1:255790/86‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                             CCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTC  >  NC_000913/155850‑156272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑