Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913883,3960CT100.0% 78.1 / NA 29intergenic (‑8/‑277)ybjG/mdfAundecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/13);  total (16/13)

AAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAA‑TAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCT  >  NC_000913/883155‑883614
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           
aaaaaGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGTTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGATAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAg                                                                                                                                                                                                                    <  1:204129/251‑1 (MQ=255)
                   naGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAAt                                                                                                                                                                                                 >  1:214137/2‑251 (MQ=255)
                                    gACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCCCTGCCAGCAAC‑GGCACCACGGTAATCAAAGCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAGGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATACCTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTgag                                                                                                                                                                                <  1:7051/251‑1 (MQ=255)
                                    gACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGCCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATCAGAGAGCATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTg                                                                                                                                                                                  >  1:170084/1‑249 (MQ=255)
                                                 aTAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGATAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAgg                                                                                                                                                                   <  1:255901/251‑1 (MQ=255)
                                                      gATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGACGTTGAGAATAAAGTGCAgg                                                                                                                                                                   >  1:6686/1‑246 (MQ=255)
                                                       aTTTTTATCGCCAGGTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGGGGTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGGCGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGATAATAAAGTGCAGTTTAAAc                                                                                                                                                             <  1:181623/251‑1 (MQ=255)
                                                                   aGTTGCCGTTGTGCTGTAAACCCCCACAACCAAAGTCCCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCTGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTGCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCgg                                                                                                                                                 <  1:7020/251‑1 (MQ=255)
                                                                            tttgCTGTAAGCCCCCACAACCAAAGTACGACGGCCAG‑AACAGGCACCCCGGTAATAAAATCTTTAGCAATAAAAATCGCCAACGGGATCATACACGGAGCCGAGTCTGGAGACGCGTTAATAAGAGAGAATAGAGCGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGc                                                                                                                                                   >  1:341465/3‑240 (MQ=255)
                                                                                         cccACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGc                                                                                                                           >  1:105764/1‑251 (MQ=255)
                                                                                                        ccACGGCCAGCAAC‑GGCACCACGGTAATCAAAGCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGGCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGc                                                                                                                           <  1:312386/236‑1 (MQ=255)
                                                                                                           cGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCACGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACga                                                                                                              <  1:61381/246‑2 (MQ=255)
                                                                                                                    aC‑GGCACCACGGTAATCAAATCTTTAGCAATAACAATAGCCAACGAGATCATCCACGGCGCCGCGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCACATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAAc                                                                                                >  1:146395/1‑251 (MQ=255)
                                                                                                                                                cAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTATGGCGTCGCGTTCATCAGAGAAAATAGAGAGAGATTCAAATTTTCCAGAATAACTTCCTGACGCACAGTGATTAAAAGGAAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGTAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAAATAAACCTTaa                                                                       >  1:187420/1‑249 (MQ=255)
                                                                                                                                                    aaaaaTCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTNGTTNNNNNTann                                                                                                                                                                          >  2:224010/1‑144 (MQ=255)
                                                                                                                                                           gCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGACTAGAGAGCGACTCACCTTTTCCAGCATAACTTCCTGACGCAAAGTGATTACAAGGGa                                                                                                                                                                                                       >  1:356654/1‑110 (MQ=255)
                                                                                                                                                            ccAACGAGCTCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCACATTTTCCAGAATCACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAA‑TAAAACTTAAAGCACGCGTAGCtat                                                        >  1:53483/1‑251 (MQ=255)
                                                                                                                                                                 gagaTCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAA‑TAAAACTTAAAGAAAGCGTAGCTATACTcn                                                   <  1:224010/251‑2 (MQ=255)
                                                                                                                                                                  agaTCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAA‑GGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGGGCAGGTTAAACTGGGTAAATCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACggtgg                                                                                                           <  1:305491/194‑1 (MQ=255)
                                                                                                                                                                                    aGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCNNTACAggnn                                                                                                                                                                                          >  2:23121/1‑96 (MQ=255)
                                                                                                                                                                                       nnnnnntCNNNNNAATNAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTc                                                                                                                                   <  2:214137/144‑1 (MQ=255)
                                                                                                                                                                                          gCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTc                                                                                                                               >  1:236150/1‑151 (MQ=255)
                                                                                                                                                                                              cgcgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCt                                                                                                                                         >  1:278954/1‑137 (MQ=255)
                                                                                                                                                                                                  gtaataaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAggg                                                                                                                                                                                                        <  1:163322/69‑1 (MQ=255)
                                                                                                                                                                                                   taataaGAGAGAATAGAGATAGAGTCAAATTTTCCAGCATAACTGCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCTTTGAGAATAAAGGGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGGTTACGGTTGGGGATTGTAACGGGCATAGGTTAAA‑TAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTc                  <  1:325643/250‑1 (MQ=255)
                                                                                                                                                                                                    aataaGAGAGAATAGAGAGAGATTCAAATTATCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAA‑TAAAACGTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAg                <  1:23121/251‑1 (MQ=255)
                                                                                                                                                                                                                  gagagagaTTCAAATTTTCCAGCATAACTTCCTGACGCCCAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGCGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAA‑TAAACCTTAAAGCAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCt  >  1:127745/1‑251 (MQ=255)
                                                                                                                                                                                                                              aaTTTTCCAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTACAGCGGCATCGTCTTATTTCCCTCCAGCGGcc                                                                                                                       >  1:199097/1‑123 (MQ=255)
                                                                                                                                                                                                                                    ccAGCATAACTTCCTGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCa                                                                                                                                               >  1:108189/1‑93 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           
AAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAAC‑GGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAA‑TAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCT  >  NC_000913/883155‑883614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑