Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	789,580	C→A	E134* (GAA→TAA)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	789,580	0	C	A	100.0%	36.6 / NA	14	E134* (GAA→TAA)	galK	galactokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/8);  total (6/8)

AAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCACGC  >  NC_000913/789382‑789786                                                                                                                                                                                                       |                                                                                                                                                                                                               naaCTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCAAGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATc                                                                                                                                                            >  1:208997/2‑251 (MQ=255)          tGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCGGCGGCACATTGCCGCTGATCACCATGTc                                                                                                                                                    <  1:113508/250‑1 (MQ=255)                                        aGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTgn                                                                                                                     <  1:84607/251‑2 (MQ=255)                                                 ctttcttGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTan                                                                                                                             <  1:217023/234‑2 (MQ=255)                                                        gCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacg                                                                                                      <  1:17265/250‑1 (MQ=255)                                                                            gATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATg                                                                                                                                                      >  1:347771/1‑182 (MQ=255)                                                                                caagATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGc                                                                                                                                                                                     <  1:21842/144‑1 (MQ=255)                                                                                       ccGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCAGTACAGCGGCAGATGCTAAAGCTGCTGCAATCCGGTTCCGACCGCGACTTACAGTGAAGAGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCa                                                                                                                          >  1:348960/1‑199 (MQ=255)                                                                                                                    tGCTTCATGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCtgttgt                                                                                                                                >  1:119706/1‑164 (MQ=255)                                                                                                                       ttCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGATGCTGCACTACGGTTAAGACCGCGAATTACAGTGAAGCGGAAGCAATTAACACGGCACCCTGCGGCACATTGGAGCTGATCACCATGACAACGCCGTCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGCAGTTAGCCCATTGATAGTTTTCATGTGCGACCATGGGCGCATCGAGGGAAAACt                                      >  1:344231/1‑251 (MQ=255)                                                                                                                                              gggcgcCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCg               >  1:331743/3‑251 (MQ=255)                                                                                                                                                           cGGCAGATGATAAAGCTGCTGCAATACGGGTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTATCTCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCTGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTGCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTGCATGTGCGACAATGGGGGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCAcgc  <  1:130064/251‑1 (MQ=255)                                                                                                                                                                              nncANNNNNGTTNCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGtt                                                                                        <  2:208997/144‑1 (MQ=255)                                                                                                                                                                                                   aCTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTGTCTCCACGGCACGAACGTAGTTAGCCTATTGATNg                                                                           <  1:275770/138‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                               AAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCACGC  >  NC_000913/789382‑789786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑