Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	154,182	C→T	L415L (CTG→CTA)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	154,182	0	C	T	100.0%	47.7 / NA	18	L415L (CTG→CTA)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

CATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACC‑TGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATG  >  NC_000913/153939‑154399                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           cATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCa                                                                                                                                                                                                                      >  1:125610/1‑250 (MQ=255)                                               aTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTATGTCGAATAGCGATAGGACGCGATATTCACTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATACCTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGCAAGAAACTGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATATGAATAcc                                                                                                                                                                       >  1:152222/1‑251 (MQ=255)                                                                            gTAATTCTGTGTCGAATAGCGATAGGCCACGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAggn                                                                                                                                          <  1:91756/251‑2 (MQ=255)                                                                                         gAATAGCGATAGGCCGCGATATTTAGTGAAGTACTTGTTTCTTCGAATAACTTGTTACAGGACACCCGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGACTGAGTCACATCGCAAGAAAATGCACCAACTGAAGTATTTAGCCCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGc                                                                                                                                          >  1:331451/1‑238 (MQ=255)                                                                                                    ggCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATAATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGGAGCTTGCTTGaaat                                                                                                                   >  1:239158/1‑250 (MQ=255)                                                                                                         cgATATTCAGTGAAGTACTTGTTTCTTCGAATAACGTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCTGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGCCCAAGACCTAACAAACCAGCGGTATAGTTAGTATCGGTTATCTTAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgtag                                                                                                                                     <  1:199470/227‑1 (MQ=255)                                                                                                                                  ttcGAATAACTTGTTCCAGGAAACACGATATCTTTGCCCCTGGTATGTTTTATCATCCGGGATATGAACATTGGAATGAGGCACATCGAAAGAAAATGCACCAGCTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTCATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTc                                                                                                                                              <  1:199357/193‑1 (MQ=255)                                                                                                                                                                     gCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAAGGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTAGCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgtagtag                                                                                                                                  <  1:184999/170‑1 (MQ=255)                                                                                                                                                                      ccccTGGTATGTTTTATCATCCGGGATCCGCACCCTGGACTGAGTCACATCGAAAGAAATTAACCCCACTGAAGAATCTAGCCCAAGCCCTAACAAACCAGCGGTGTCGTTTTTATCGGTTATCTGTCTACCGCTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATACATTAGGCTCATCCTGAat                                                                                               >  1:147178/1‑204 (MQ=255)                                                                                                                                                                      ccccTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATc                                                                                                                                                                               <  1:125580/124‑1 (MQ=255)                                                                                                                                                                                  ttnATCNTNNGNNATANGAACATTGGAATGAGTCACATNNAAAGAAAATNCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTANCGGTTATCTNNATNCCGGGNTNNNCNNNCANATAGTTATTCAGGCCGTAGNAGNNGCTTGCNTGAAATAAATTAGGCTCATCCTGAat                                                                                               <  2:77411/192‑1 (MQ=255)                                                                                                                                                                                           ctggATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACC‑TGACCGCCGCTAATATCCCACCGTCCAACGCCAGGGCGTAACATTTGaaca                           >  1:322616/3‑250 (MQ=255)                                                                                                                                                                                           cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAGCAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACC‑TGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaaca                           >  1:128710/1‑250 (MQ=255)                                                                                                                                                                                              naTACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCACGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACC‑TGACCGCCGCTAATATCACAACGTCCAACGCCAGGGCGTAACATTTGAACAACgg                       >  1:39759/2‑251 (MQ=255)                                                                                                                                                                                                          gtaaTGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACC‑TGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGtt           >  1:339608/3‑251 (MQ=255)                                                                                                                                                                                                                   aacaTCGAAAGAAAATGCACCACCTGAAGTATTTGAACCAACACCTAAAAACCCAGGTGTATAGTTATTATCGGTTGTCGGAATCCCGTTATACCCCTTGAAATAGTTATTCAGGCCGGAATAGTAGCTTGGGTAAAATAAATTAGCCGCATGTTGAATATTATCTTGTTATACCGTG‑CCGCCGCTAAGATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATg  <  1:316479/250‑1 (MQ=255)                                                                                                                                                                                                                                      cnAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATatcat                                                                                          >  1:278501/1‑145 (MQ=255)                                                                                                                                                                                                                                               gTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACC‑Tg                                                                            <  1:20175/149‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           CATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACC‑TGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATG  >  NC_000913/153939‑154399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑