Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	753,977	G→A	R164R (CGC→CGT)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,977	0	G	A	100.0%	26.7 / NA	11	R164R (CGC→CGT)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/8);  total (3/8)

CGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACC  >  NC_000913/753769‑754192                                                                                                                                                                                                                 |                                                                                                                                                                                                                         cGTTCTGTTCATGGTCAGAGTGCAGGATCAGAATAAGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGACGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATcc                                                                                                                                                                               >  1:297882/1‑251 (MQ=255)       gTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACTTTCCAGAATCGGATTAACTTCATACGGTTCGCCCGGCTTGGAGAACATCATATTCAGGAAGTAACCGGCGTAGGAGAGATCGTTGCGCGGGTAAGCAAATGGCGGCCCAATGGATTACTTGTA‑CACATCTCGGCCATTGTCTGCATTTTCTACAGCAGACGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAg                                                                                                                                                                         <  1:236667/250‑1 (MQ=255)              gTCAGCGTGCAGTATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGTAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCGGGCCAATGTAATACTTTTAACACATCGCGGCCATGTTCGGCATTTGCGACAGCAGACGGAATGCGGCAATTGCACGGTG‑ACGATGGTTGTTAACATCCAGCGAGTCGTGAt                                                                                                                                                                  <  1:141340/251‑1 (MQ=255)                                aattcGGTACATAGCATGTTCCAGAATCGGATTAACTTCATACGTTTCGCACGGCGTGGAGAACATCATAGTCAGGAATGTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGATCAATGGAATACTTGTAACCCATCGCGGCCAGTGTCGGCAGTGGGGACTGCATACGGAACGCGGCAATTTCGCG‑TGTAGGAGGATTGTTAACAGCCAGCGAGTCGTGATAGAACGCCGTCAgcgcgc                                                                                                                                                <  1:249005/247‑1 (MQ=255)                                                                                         ngagaACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGACGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAAc                                                                                        >  1:284485/2‑250 (MQ=255)                                                                                          gagaACAGCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGACGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAcgc                                                                                                                                                           <  1:127251/182‑1 (MQ=255)                                                                                                                             gagaTCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGACGGAACGTGGCAATTTCACGGTG‑ACGAGGATTGCTAACCTCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACGGCCATAGGGTGCGAGTCGCGACGGAAAGCATGGAACATACGGGTAATCTGCTCGTGGATCATGGTATGACggn                                                   <  1:30554/251‑2 (MQ=255)                                                                                                                                                                         tGTAACACATCGCGGGCATGGTCGGCATTTTAGACAGCAGACGGAACGAGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATgg                                                                                           >  1:296721/1‑167 (MQ=255)                                                                                                                                                                              cacaTCGCGGCCATGGTCGGCATTTTCGACAGCAGACGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCAcc  <  1:273666/251‑1 (MQ=255)                                                                                                                                                                                    gcgGCCATGGTCGGCATTTTCGACAGCAGACGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTc                                                                       <  1:24267/176‑1 (MQ=255)                                                                                                                                                                                                ggCATTTTCGACAGCAGACGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACAGCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGAGGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTc                                                 <  1:231867/186‑1 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                         CGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGGTG‑ACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACC  >  NC_000913/753769‑754192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑