Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	721,604	G→A	R44C (CGT→TGT)	kdpE ←	DNA‑binding transcriptional activator KdpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	721,604	0	G	A	100.0%	20.0 / NA	8	R44C (CGT→TGT)	kdpE	DNA‑binding transcriptional activator KdpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

GCGCGGTGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTG  >  NC_000913/721361‑721782                                                                                                                                                                                                                                                    |                                                                                                                                                                                   gcgcGGTGGTGGCAGAGTGGCGGCGTACTGCGACGCGCAGACGGGCCTGCCATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCCATCCCATCACCATCTGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGcc                                                                                                                                                                             >  1:30107/1‑251 (MQ=255)   gcgGTGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGc                                                                                                                                                                           <  1:55834/251‑1 (MQ=255)                                                        gCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGc                                                                                                                                                                           <  1:146350/198‑1 (MQ=255)                                                                                                             cgATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCAGCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCGTACAGGTTGCCGGTTCCAGCAAGCCGCGTTGCAGCg                                                                                                                                                    <  1:290907/168‑1 (MQ=255)                                                                                                                                               cacaATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGt                                                                                                                                                                                 <  1:204019/105‑1 (MQ=255)                                                                                                                                                                    ccACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGCGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTg          >  1:155144/1‑251 (MQ=255)                                                                                                                                                                         ggCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGCATAATAAAATCTGGCTTACAGGATGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAGAGACGCGCATCACGTCGCCCTCCAGCGCCGTGCGCAGCAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCCCATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGa      >  1:142442/1‑250 (MQ=255)                                                                                                                                                                            gcAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGACGCTTCAAGCAAGCAGCGTTGCAGCGCTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTAACATATACTCATGAAATTCTTCAAGTTAAGGGGCAGTTTGCTGGGGAAgtg  >  1:263422/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                   GCGCGGTGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTG  >  NC_000913/721361‑721782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑