Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	627,348	G→A	V427M (GTG→ATG)	entE →	2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	627,348	0	G	A	100.0%	32.8 / NA	14	V427M (GTG→ATG)	entE	2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

ATAGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTG  >  NC_000913/627106‑627564                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          atAGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAATGATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAgggn                                                                                                                                                                                                                   <  1:68962/251‑2 (MQ=255)                              ggTTACCCAATGTGTCCGGATGACGCAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCA‑GATTAACCGTggcgg                                                                                                                                                                                     >  1:289615/1‑251 (MQ=255)                                                  ngACGAAGTATGGGTTGCCGATGCAGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGCCGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGTGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGa                                                                                                                                                                   >  1:103780/2‑249 (MQ=255)                                                                      atgccgaAGGAAATCCACTGCCGCAAGGGGCAGTCGGCCGCATGATGACGCGCGGGCCGTACCCCTTCCGCGGCTATTCCCCAAGTCCCCAGCACACTGCCATCGCCTTTGATGCCACCGGTTTTTACTGTTCCGGCGTTCTGATCTCTATTGATCCAGCGGGTTGCATCTCCATGCCGGGGCGCGAGAACGATCA‑GATTCACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACctgctgctg                                                                                                                                             >  1:164737/1‑251 (MQ=255)                                                                         ccgaAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTgcgc                                                                                                                                          <  1:224940/251‑1 (MQ=255)                                                                                                              ccTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGATTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCNTGcnnnnnggnn                                                                                                                                                                                                               >  2:222540/1‑137 (MQ=255)                                                                                                                            ggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGCTCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGCGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATg                                                                                       >  1:203957/1‑251 (MQ=255)                                                                                                                                                               caGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCNNGGgcgcn                                                                                                                                                                                                               >  2:23730/1‑96 (MQ=255)                                                                                                                                                               caGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGc                                                                           <  1:23730/228‑1 (MQ=255)                                                                                                                                                                    caATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGTAAACCTGCTGCTGCGCCATCCGGCGGTGATCTACGGCGCACTTGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAATAGCCGc                                               <  1:245379/251‑1 (MQ=255)                                                                                                                                                                    caATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGCGGCGCGAGAAAGATCA‑GATTAACCg                                                                                                                                                                                           <  1:95879/111‑1 (MQ=255)                                                                                                                                                                                                              cGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCA‑GATTACCCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAATCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAgggg      >  1:6330/1‑249 (MQ=255)                                                                                                                                                                                                                    gatctCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCCCCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTAttn  <  1:222540/248‑2 (MQ=255)                                                                                                                                                                                                                       ctctATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGA‑CACGCTTACCCGTGGCGGCGCGAACACCGCTGCCGAACCGATCGCCAACCTGCTGCTGCGCCACCCGGCAGTGATCCACGCCGCACTGGTGAGCATGGAAGATGAGCTCATGGGCGCAAACACCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCACGGTGCAGGTGCGTCGtt                       >  1:157748/1‑224 (MQ=255)                                                                                                                                                                                                                              gacccAGAGGGTAACATCACCATGCAGGGGCGCGAGAAAGATCA‑GATTAACCTTGGCGGCGAGAAGATCGCTTCCGAATAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGAGAGCa                                                                                                        <  1:351360/133‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          ATAGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCA‑GATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTG  >  NC_000913/627106‑627564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑