Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	582,597	C→T	intergenic (+500/‑278)	tfaD → / → tfaX	putative tail fiber assembly protein TfaD/protein TfaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	582,597	0	C	T	90.9%	17.2 / ‑3.3	11	intergenic (+500/‑278)	tfaD/tfaX	putative tail fiber assembly protein TfaD/protein TfaX
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (5/5);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

GTATAGAGTTCCATCGTCAGTTAAGGCCTGCGCCGCATTGCGTATTACACAGCTTTTTGTAGATATATTTCCAGGCAGGCAGTGAAGAAGGTAAGACATGGAAATGGAATCAAATTGACCATGTAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGTTTAATTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGACTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGTTCCAACACCAATATCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTGTTCCTTTGTAGGACATCCCCATGCAAGCCGATTTGATACTCCCAAAACCCACCAGTCATAAAGCTTTAGGGTAAGTGGTGTGTAAATTCTAGCCCCATC  >  NC_000913/582353‑582790                                                                                                                                                                                                                                                     |                                                                                                                                                                                                  gTATAGAGTTCCATCGTCAGTTAAGGCCTGCGCCGCATTGCGTATTACACAGCTTTTTGTAGATATATTTCCAGGCAGGCAGTGAAGAAGGTAAGACATGGAAATGGAATCAAATTGACCATGTAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGTTTAATTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGATTActct                                                                                                                                                                                             >  1:231599/1‑251 (MQ=255)                gTCAGTTAAGGCCTGCGCCGCCTTGCGTATTACACAGCTTTTTGTAGATATATTTCCAGGCACGCAGTGCAGAAGGTAAGACATGGAAATGGAATCAAATTGACCATGTAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGTTTACTTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGATTACTCTCAGGTACGTGAGTa                                                                                                                                                                               >  1:140514/1‑250 (MQ=255)                          gCCTGCGCCGCATTGCGTCTTACCCACCTTTTTGTAGATATATTTCCAGGCAGGCAGTGAAGAAGGTAAGACATGGAAATGGAATCACATTGACCATGTAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGTTTAATTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGACTACTCTCAGGTACGTGAGAAAGGTAAAAccc                                                                                                                                                                    >  1:118955/1‑251 (MQ=255)                                   gcATTGCGTATTACACAGCTTTTTGTAGATATATTTCCAGGCAGGCAGTGAAGAAGGTAAGACATGGAAATGGAATCAAATTGACCATGTAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGTTTAATTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGATTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGTTCCAac                                                                                                                                                           <  1:28281/251‑1 (MQ=255)                                                                                                 cATGGAAATGGAATCAAATTGACCATGTAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGTTTAATTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGGTCAAATCCATTAAAGATATCAGATTTCGCGCAGGTACGTGATTAATGTAAAACCCAGTTCCAACACCAATAGCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTGTTCCTTTg                                                                                             <  1:247417/251‑1 (MQ=255)                                                                                                                     gACCATGGAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGGTTAATTTTTGATTCCCCAGCCCTTGTAGATGCCTCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGATTACTCTCAGGGTCGTGAGTAAGGTAAAACCCAGTTCTAACACCAATATCCAGATGCTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTTTGCCTTTGTAGGACATCCCCATGCAAg                                                                          <  1:323058/250‑1 (MQ=255)                                                                                                                                                                          ttAATTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGATTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGGTCCAACACCAATATCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTGTTCCTTTGTAGGGCATCCCCATGCAATCCGATTTGATACTCCCAAAACCCACCAGTCATAAAGCTTTAGGGTAAgtggtg                     <  1:57451/250‑1 (MQ=255)                                                                                                                                                                                              ccTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCAATTAAAGATATCAGATTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGTTCCAACACCAATATCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTGTTCCTTTGTAGGACATCCCCATGCAAGCCGATTTGATACTCCCAAAACCCACCAGTCATAAAGCTTTAGGGTAAGTGGTGTGTAAATTCTAGccc      >  1:288051/1‑245 (MQ=255)                                                                                                                                                                                                         ccGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGATTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGTTCCAACACCAATATCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGNAGGNNNNNCtnnnnnn                                                                                          >  2:217513/1‑144 (MQ=255)                                                                                                                                                                                                                       gCTTCGTTCAAATCCATTAAAGATATCAGATTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGTTCCAACACCAATATCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTGTTCCTTTGTAGGACATCCCCATGCAAGCCGATTTGATACTCCCACAACCCACCAGTCATaaa                                       >  1:118129/1‑187 (MQ=255)                                                                                                                                                                                                                            gTTCAAATCCATTAAAGATATCAGATTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGTTCCAACACCAATATCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTGTTCCTTTGTAGGACATCCCCATGCAAGCCGATTTGATACTCCCAAAACCCACCAGTCATAAAGCGTTAGGGTAAGTGGTGTGTAAATTCTAGCCCcatc  <  1:124499/219‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                  GTATAGAGTTCCATCGTCAGTTAAGGCCTGCGCCGCATTGCGTATTACACAGCTTTTTGTAGATATATTTCCAGGCAGGCAGTGAAGAAGGTAAGACATGGAAATGGAATCAAATTGACCATGTAACGCCGCGGGATAAGGTTCAAAAACATCATGGCTAATTTTATGTTTAATTTTTGATTCCCCAGCCCTTGTAGATGCCGCGTTCAGGCTAGCTTCGTTCAAATCCATTAAAGATATCAGACTACTCTCAGGTACGTGAGTAAGGTAAAACCCAGTTCCAACACCAATATCCAGATGGTTGTTACCTAAATGTTCCAGAAAGTGTGGAAGAAGGTGTTCCTTTGTAGGACATCCCCATGCAAGCCGATTTGATACTCCCAAAACCCACCAGTCATAAAGCTTTAGGGTAAGTGGTGTGTAAATTCTAGCCCCATC  >  NC_000913/582353‑582790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑