Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,742	G→A	G276D (GGT→GAT)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,742	0	G	A	100.0%	52.0 / NA	19	G276D (GGT→GAT)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/12);  total (7/12)

GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGG  >  NC_000913/534550‑534960                                                                                                                                                                                                 |                                                                                                                                                                                                                           gCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCCTACCGGCTCGGTAGCGAACTACCCCGAAggg                                                                                                                                                                  >  1:55820/1‑251 (MQ=255)    gggTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGgcgn                                                                                                                                                               <  1:40679/251‑2 (MQ=255)    gggTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCAAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGGGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCAGACCGGCTCGGTAGAGAAATACACCGAAGGgcgc                                                                                                                                                               <  1:290924/251‑1 (MQ=255)                cTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCg                                                                                                                                                                                       <  1:140289/215‑1 (MQ=255)                           gCACTGTTACAACAGTTGGCTGATCTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAAGTGATGGCCGGGATGGTTGGTCTGGAAACCGCGCATCGTTACGGTAACGCAAGGCTGCTGGCGTCGGACATGGTGTTTGATAGCGGTAAGCGTTGTGCTAACCGTCATTCCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgat                                                                                                                                             <  1:162822/246‑1 (MQ=255)                              ntGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATCCCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGcc                                                                                                                                     >  1:73765/2‑251 (MQ=255)                                gTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCTTTTTGCTAACCGTCAGACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGcn                                                                                                                                     <  1:40253/249‑2 (MQ=255)                                           ttGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTgn                                                                                                                         <  1:221723/250‑2 (MQ=255)                                                               ccGGTGATCCCAACGCTCATGGGCTGGGGCTGTATCCCGGACGATCATGAACGGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCCACTCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCACACCGGCTCGGTAGATAAATACACCGAAGGCCGCAAAATCGTTCATATTGATATTGAGCCGACTCAAATTGGTCGCGTGCTGTGTCCGGAtc                                                                                                     <  1:344081/250‑1 (MQ=255)                                                                   tGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGGTGGAGGGGCTGCACACCGCGAATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTACCCGTTTTGCTAACAGTCATAACGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCggg                                                                                                >  1:185844/1‑250 (MQ=255)                                                                                          ggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAgcggn                                                                         <  1:92788/251‑2 (MQ=255)                                                                                                                    gNTNNCNNGGANGGTGGGTCTGCAAACCGCGCANNGTTACGGTANCGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTANCCGTCAGACNNGCNCGGTANANNNANNNACNGAAGGGCGCAAAATCGTTCNTANNGATATTNAGCCGACGCAAATTGGTCGCGTGCtg                                                                                                               <  2:78901/187‑1 (MQ=255)                                                                                                                            cagacgtgtgctcttcccgatctcATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATg                                       <  1:13619/228‑1 (MQ=255)                                                                                                                                 gagggTATGCAAACCGCGCCTCGTTACGGTGACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCgg                                                                                                 >  1:302421/3‑188 (MQ=255)                                                                                                                                         ncAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCCTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTAGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTACGGAtc                                                                                                     >  1:41164/2‑176 (MQ=255)                                                                                                                                                   cATCGTTACGGTACCGCAACGCTGATGGCGTCTGACCTGGCGTTTGATATCGGTAACCGTTTTGCTAACCGTc                                                                                                                                                                                                  >  1:110766/1‑73 (MQ=255)                                                                                                                                                     tCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATAAACCGAAGGGAGCAAAATCGTTCatattgat                                                                                                                                             >  1:147138/1‑124 (MQ=255)                                                                                                                                                             gTAACGCAACGCTGCTGGCGTCTGACATGGTGTTGGAGATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACGCCGGAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTc                                                                                                           <  1:52820/150‑1 (MQ=255)                                                                                                                                                                 cgcaacgcTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATggg  <  1:143252/251‑1 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                                           GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGG  >  NC_000913/534550‑534960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑