| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 81,102 | G→A | S286L (TCG→TTG) | leuB ← | 3‑isopropylmalate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 81,102 | 0 | G | A | 94.7% | 42.8 / ‑6.4 | 19 | S286L (TCG→TTG) | leuB | 3‑isopropylmalate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (12/6); total (13/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTA > NC_000913/80862‑81332 | cATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGAGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGCAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGccgcccgc > 1:247025/1‑251 (MQ=255) cATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAACTCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCCCTGCCGCAAGCGGCATCATCGCCATCCAGGCTGTAACGCAGGAGCAGGGCCAGCGACAGGATTTTTGCAATTGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGCTCATACAGTCCAAAACCTTGCTCGCTCAGGCTGGCGGa > 1:195564/1‑249 (MQ=255) tgtCATCGGTACTAACGGGGGCAGCGCCACGGGGTAAATCCCCGGTGGGAATGCCTTTTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCTATGTTTTTTCCAGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAgg < 1:301404/248‑1 (MQ=255) gcAGCGCCACGGGCTAAATCCCCAGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAACCGCATCATCGGCATCCAGGCTGTAACGCAGCACCAGTGACAGCGAACGGATTTGTGCAATCGGGATGGCGATGTTTTTGCATGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTACAGGCTGCCGGAAGGCAACCTCCCCATCTAGCCAgg > 1:132642/1‑250 (MQ=255) aGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAAGGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAAGACAGCAGCAGTGCCAGCGAATGGAGTTGTGCAACCGGGTTGGCGATGTTTTTGCCTGCGATATCGGGGGCCAGGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCGCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTg < 1:346259/250‑1 (MQ=255) cccGGGCTAAATCCCCGGTGCGAATTCTTTTTTCTAATCCGCGTTTAAGGGGGCTGTCAATGGCGCAAGTCGCATCCTCGGCATCCAGGCTGTACCGCGGCGGTAGTTCCATCGAAAGGATTTGGGCTATCGGGTTGTCGATTTTTTTGCCTGCTATATCTGGTGCCAACCCGTCCGCCGGTTCATACAGTCCAAAACCTGGCTCGTTCAGGCTGGCTGAAGTCTACATCCCCACCGAGCCGGTGATCAtt < 1:227275/249‑1 (MQ=255) gCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAACACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTgcgc > 1:153990/1‑250 (MQ=255) cccGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATACAGGCTGTAACGCAGCAGCAGTGCCAGCGAACGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAgccgc > 1:49656/1‑161 (MQ=255) gAATGCCTTCTCCTAATGCTCGGTTATTGGCGCGTTCAATGGCGCAACCCGCATCATCGGCTTCCTGGTTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGGAATCGTTTTGCCGATATTTTTGCCTGCAATATCTTGTGCCAGGCCCCCCGCCGGTTCATACATTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCACCATcc < 1:177755/210‑1 (MQ=255) ccTTCTTCAAATGCGCGGTTAATGGCTGGTCCAATGGCGCAAGCCGCAGCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGTATTTGTGCAATGGGTTTGGCGATGTTTTTGCCTGCGATAGCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCTTTCCAGTGATCATTGCGCACTCGGCAGACAGAATGTCGcc < 1:245878/251‑1 (MQ=255) gcgcGGTTAATGGCGCGTTCAATGCCTCAAGCCGCATCATCGGCCTCCAGGCTGTAACGAAGCAGCAGTGCCAGCGAAAGGATTTGGGCAATCGGGTTGGCGATGTTTTTTCCTGCGATATCTGTTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCAGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGa < 1:52547/251‑1 (MQ=255) cgTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATCTCTGGTGCCAAGCCGCCCGCCGGTTCATCCAGTCCACAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATcc > 1:173321/1‑178 (MQ=255) gcgcAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGAGAAAGGATTTATGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCAAAAACCTTGCTCGTTCCGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCACAACAGGTTGGAGCACAGCAGAACGTCAAACtgtg > 1:259271/1‑250 (MQ=255) gTAACGCAGCAGCAGCGCCAGCGAAAGGATTTGTGCACGCGGGTTGGCGATGTTTTTGCCTGCGATATCTACTGCCAAGCCGCCCGCCGGTTCATACAGTCAACAACCTTGCTCGTTCCGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGATGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCTGCTGCATGGTGGCGt > 1:266128/1‑251 (MQ=255) nccAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATg > 1:36344/2‑251 (MQ=255) ccAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATgc > 1:139905/1‑251 (MQ=255) gCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGCACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTa > 1:163288/1‑250 (MQ=255) gCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAAACTTGCTCGGTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGACGTTGTCGATGTACATATGAGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCggta > 1:267136/1‑247 (MQ=255) cTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCCTCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATgcgc > 1:52904/1‑202 (MQ=255) | CATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTA > NC_000913/80862‑81332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |