Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,680	C→T	F187F (TTC→TTT)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,680	0	C	T	100.0%	56.1 / NA	20	F187F (TTC→TTT)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

CCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAG  >  NC_000913/495434‑495861                                                                                                                                                                                                                                                       |                                                                                                                                                                                      ccTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTGCTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATTCACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTtctc                                                                                                                                                                                    <  1:142069/250‑1 (MQ=255)                            gAAAGACAGCCAGCTGATCGGTCATGTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCATAAAAGGGCGTCTGCTGGGAATCGGCTGTCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCAGCTGCATGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCAtcatca                                                                                                                                                        <  1:318493/251‑1 (MQ=255)                                                           gtTGGTTTCTACTCTGCGGTTATCGTGTCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACAGCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGGTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGt                                                                                                                         <  1:305296/251‑1 (MQ=255)                                                               gTTTCTACTCTGCGTTTATCGTGGCCGACAAAGGGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATAAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGACGGCGCAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTag                                                                                                                       >  1:168107/1‑249 (MQ=255)                                                                      ctctGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGaa                                                                                                               >  1:291005/1‑250 (MQ=255)                                                                                                       gCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTAtct                                                                             >  1:94711/1‑251 (MQ=255)                                                                                                            cTCGCGCGGCAGGCGAAAAACCAGAAACTGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCATTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTgg                                                                        >  1:291814/1‑251 (MQ=255)                                                                                                              cgcgcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTgg                                                                        <  1:301004/249‑1 (MQ=255)                                                                                                                cgcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGACTCGGCTGGCGCGGGTGAATACATCGTTGACGCCATCATCAAAGAAGCTCGTGCTACTGAAATCACCCTGCCTCTGCGTGACGGCGACGACCTGTTTCTCGTTGACTGGCGCGTGCGTTCCATCATCAGCAACTACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAACCGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTggg                                                                       >  1:247889/1‑248 (MQ=255)                                                                                                                    gCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAt                                                                >  1:4320/1‑251 (MQ=255)                                                                                                                    gCAGGCGAAAAACCAGAAAATGGCGTCTTCAGGGAATCGGCTGTCGAAGTTGATTACTCCGGGGCCGACATCAGCAAAGAAGATCGTGGTAGTGTAATCTCCGTGCATCTGCGTAAAGGCTATGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCa                                                                                                                                        <  1:346295/179‑1 (MQ=255)                                                                                                                     cAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCAGGCGAAGATGAATACACCGTAGCAGACATATTCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGGTCGAAAAACGCGACGAGAAAGACGGCGAAACCGTTATCTCCTGGGCGAAAATc                                                               >  1:238947/1‑251 (MQ=255)                                                                                                                             aaaCCAGAAAATGGCGTCTGCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGGGCGTTCCATCATCAGCAAAAACTCCGACCATATCGCGCTGCCGGTAGATATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAgc                                                       <  1:48365/251‑1 (MQ=255)                                                                                                                                                    ggAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAAc                                 >  1:113879/1‑250 (MQ=255)                                                                                                                                                          ggCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACATAAATCTCCCTGCATCTGCGGGAAGGCTAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATGCTCCGGCCAGATCGCGCTTCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCt                                                                          <  1:360110/203‑1 (MQ=255)                                                                                                                                                                cGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGgcgcn                                                                                                                                                                       <  1:218796/104‑2 (MQ=255)                                                                                                                                                                cGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGcgcg                                                                                                                                                                       >  2:218796/1‑104 (MQ=255)                                                                                                                                                                                   gCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAGCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAag  <  1:152127/250‑1 (MQ=255)                                                                                                                                                                                         atcatcAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATan                                                                                                                                                <  1:63918/102‑2 (MQ=255)                                                                                                                                                                                                                      cACCCTGCATCTGCGTGAAGGCGAAGACGAGTTTCTCGATGACTGGCGCGTGCGGTAAATCATCCGCAAATACTCCGACCACATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGCGAAAGACGGCGAAACAGTTATCTCCTGGGAGCAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTAc      >  1:162984/1‑211 (MQ=255)                                                                                                                                                                                                                                                 cGAGTTTCTCGGTGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAACCCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCg                           >  1:298663/1‑163 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                      CCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAG  >  NC_000913/495434‑495861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑