Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	471,709	G→A	K358K (AAG→AAA)	mdlB →	ABC transporter family protein MdlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	471,709	0	G	A	100.0%	30.2 / NA	13	K358K (AAG→AAA)	mdlB	ABC transporter family protein MdlB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

GGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGG  >  NC_000913/471479‑471882                                                                                                                                                                                                                                       |                                                                                                                                                                              ggCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGcc                                                                                                                                                           <  1:22979/251‑1 (MQ=255)                                       tAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCGATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGc                                                                                                                      <  1:355027/250‑1 (MQ=255)                                                      cGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCGGGTGAGCGCGTCTTTGAACTGATGGACGGTCCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCAGCGAAGTCGAGAACGTGTCATTTGCTTATCGCGATGACAAGCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGGGGCGCTGGGCGGGCAGACCGGCAGTGGCAAAAGCAccc                                                                                                      <  1:321289/251‑1 (MQ=255)                                                       naaCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGTTCGGGCATACCGGCAGTGTCAAAAGCACCCt                                                                                                     >  1:216425/2‑251 (MQ=255)                                                       gAACTGACCACGCAACAGGAGATGATGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCg                                                                                                                        >  1:301231/1‑232 (MQ=255)                                                        aaCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAATCATTAATCTCTCTGTGCCTTCGCGCAATTTTGGGGCGCTg                                                                                                                                     <  1:295101/218‑1 (MQ=255)                                                              ccACGCAACAGGCGATGCTGCACCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt                                                                                              >  1:125516/1‑251 (MQ=255)                                                                                    aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCTCGCAATTTTGTTGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTTATGGGCTATTACCCGc                                                                        <  1:235275/251‑1 (MQ=255)                                                                                                                               ggacCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATCACGTGCCATTTGCTTATCGCGATGAAAATCTGGAGCTAAAAAACATTAATCTCTCTGTGCCTTcg                                                                                                                                                       >  1:238234/1‑129 (MQ=255)                                                                                                                                                          gatCGCCCGGTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTTGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAggg  <  1:335365/251‑1 (MQ=255)                                                                                                                                                                 cGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCCTTTGCTTATCGCGATGACAATCTGGTGCTACCAAACATTAATCTCTCTGTGCCTTAGCGCAATTTTGTGgcgc                                                                                                                                       >  1:160544/1‑111 (MQ=255)                                                                                                                                                                                      aaGTCGATNANNNGNNATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCNCTCTGTGCCTTCGCGNAANTTTGNGGCNCTGGTCGGGCATACCGGCAGTgg                                                                                                                 >  2:245496/1‑112 (MQ=255)                                                                                                                                                                                        ntCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAATGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTcc                                      >  1:66065/2‑185 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                              GGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGG  >  NC_000913/471479‑471882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑